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The map label for this gene is bioB

Identifier: 170751448

GI number: 170751448

Start: 5382747

End: 5383760

Strand: Reverse

Name: bioB

Synonym: Mrad2831_5067

Alternate gene names: 170751448

Gene position: 5383760-5382747 (Counterclockwise)

Preceding gene: 170751449

Following gene: 170751443

Centisome position: 88.58

GC content: 72.09

Gene sequence:

>1014_bases
ATGAGCGCGACTTCCCTCCCCGGTTCCCAGCAGCCTGACCTCGGCGAGATCCGCCACGACTGGAGCGTGGCGGAGATCCG
CGCGATCCACGACCTGCCGCTGATGGACCTCGTGTTCCGGGCTGCACAGGTGCACCGCCGGCACAACGACCCCGCCGACA
TCCAGCGGGCGTCGCTCCTGTCGATCAAGACCGGCGGCTGCCCGGAGGATTGCGCCTACTGCCCGCAATCGGCCCACCAC
AAGGGCGCGGGCGTCGCCCGCGAGCGGTTGATGCCGGTGGAGACGGTGCTGGCCGAGGCGGCCGCCGCCAAGGCCGCCGG
CGCCCAGCGCTTCTGCATGGGCGCCGCGTGGCGCCAGCCGAAGGACGGCCCGGAATTCGACGCGGTGCTCGCCATGGTGC
GCGGCGTGCGCGGCCTCGGCATGGAGGCCTGCGTGACGCTCGGCATGCTCACGCCCTCGCAGGCCGGGCGCCTCGCCGAG
GCCGGGCTGACCTCCTACAACCACAACCTCGACACCGGCCCGGACTTCTACGAGGACATCATCTCCACCCGGACCTACGA
CGAGCGTCTGCAGACCCTCGCGAACGTCCGCGAGGCCGGGATCGGCGTGTGCTGCGGCGGCATCGTCGGCATGGGCGAGG
GCGTCACCGACCGCGCCGCGATGCTCCAGGTGCTCGCCAACCACGCCCCCCATCCGGAGAGCGTGCCGATCAACGCCCTC
GTGGCGGTGCCCGGCACGCCGCTGGAGGATCAGCCGGCGGTCGATCCGTTCGATCTCGTGCGCATGTGCGCCACGGCGCG
GATCGTGATGCCGCGCTCCCGCGTGCGGCTCAGCGCCGGCCGCAAGAGCCTCACCCGCGAGGCCCAGGTTCTGTGCTTCC
TCGCCGGTGCCAACTCGATCTTCTACGGCGAGCGGCTGCTCACCACGGCCAATAACGGCCAGGACGACGACGCGGCTCTG
CTGGCCGATCTAGGCATCCGGGTCGGCGAGCCCGTCCGGGCCGCGGCCGAGTAG

Upstream 100 bases:

>100_bases
CTGGGCTGGTACGGCATGATCGTCCAGACCGACCCCTCGGTCGGGCGTTCGGGCGCGGGGCCCGCTTGATTTCTGGCCGC
CGATCCGGCTCAAACACCGG

Downstream 100 bases:

>100_bases
GCAACAGGTCGCCGTCTCGCCCTTCCCGGCCCCTGCGCGCGGCGCCTCAGCGGCGCCACGGAGCCCGGCATCGCCCCCCG
ACCGGGCCCCTCCCGGTTCA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSATSLPGSQQPDLGEIRHDWSVAEIRAIHDLPLMDLVFRAAQVHRRHNDPADIQRASLLSIKTGGCPEDCAYCPQSAHH
KGAGVARERLMPVETVLAEAAAAKAAGAQRFCMGAAWRQPKDGPEFDAVLAMVRGVRGLGMEACVTLGMLTPSQAGRLAE
AGLTSYNHNLDTGPDFYEDIISTRTYDERLQTLANVREAGIGVCCGGIVGMGEGVTDRAAMLQVLANHAPHPESVPINAL
VAVPGTPLEDQPAVDPFDLVRMCATARIVMPRSRVRLSAGRKSLTREAQVLCFLAGANSIFYGERLLTTANNGQDDDAAL
LADLGIRVGEPVRAAAE

Sequences:

>Translated_337_residues
MSATSLPGSQQPDLGEIRHDWSVAEIRAIHDLPLMDLVFRAAQVHRRHNDPADIQRASLLSIKTGGCPEDCAYCPQSAHH
KGAGVARERLMPVETVLAEAAAAKAAGAQRFCMGAAWRQPKDGPEFDAVLAMVRGVRGLGMEACVTLGMLTPSQAGRLAE
AGLTSYNHNLDTGPDFYEDIISTRTYDERLQTLANVREAGIGVCCGGIVGMGEGVTDRAAMLQVLANHAPHPESVPINAL
VAVPGTPLEDQPAVDPFDLVRMCATARIVMPRSRVRLSAGRKSLTREAQVLCFLAGANSIFYGERLLTTANNGQDDDAAL
LADLGIRVGEPVRAAAE
>Mature_336_residues
SATSLPGSQQPDLGEIRHDWSVAEIRAIHDLPLMDLVFRAAQVHRRHNDPADIQRASLLSIKTGGCPEDCAYCPQSAHHK
GAGVARERLMPVETVLAEAAAAKAAGAQRFCMGAAWRQPKDGPEFDAVLAMVRGVRGLGMEACVTLGMLTPSQAGRLAEA
GLTSYNHNLDTGPDFYEDIISTRTYDERLQTLANVREAGIGVCCGGIVGMGEGVTDRAAMLQVLANHAPHPESVPINALV
AVPGTPLEDQPAVDPFDLVRMCATARIVMPRSRVRLSAGRKSLTREAQVLCFLAGANSIFYGERLLTTANNGQDDDAALL
ADLGIRVGEPVRAAAE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=57.8778135048232, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321725, Length=328, Percent_Identity=45.7317073170732, Blast_Score=315, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_METRJ (B1LV19)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757708.1
- ProteinModelPortal:   B1LV19
- SMR:   B1LV19
- GeneID:   6141135
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_5067
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2331944
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35894; Mature: 35763

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSLPGSQQPDLGEIRHDWSVAEIRAIHDLPLMDLVFRAAQVHRRHNDPADIQRASLL
CCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHE
SIKTGGCPEDCAYCPQSAHHKGAGVARERLMPVETVLAEAAAAKAAGAQRFCMGAAWRQP
EEECCCCCHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
KDGPEFDAVLAMVRGVRGLGMEACVTLGMLTPSQAGRLAEAGLTSYNHNLDTGPDFYEDI
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCHHHHHHHHHCCCCCCCCHHHHHHH
ISTRTYDERLQTLANVREAGIGVCCGGIVGMGEGVTDRAAMLQVLANHAPHPESVPINAL
HHCCCHHHHHHHHHHHHHHCCCEECCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
VAVPGTPLEDQPAVDPFDLVRMCATARIVMPRSRVRLSAGRKSLTREAQVLCFLAGANSI
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCE
FYGERLLTTANNGQDDDAALLADLGIRVGEPVRAAAE
EECCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
SATSLPGSQQPDLGEIRHDWSVAEIRAIHDLPLMDLVFRAAQVHRRHNDPADIQRASLL
CCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHE
SIKTGGCPEDCAYCPQSAHHKGAGVARERLMPVETVLAEAAAAKAAGAQRFCMGAAWRQP
EEECCCCCHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
KDGPEFDAVLAMVRGVRGLGMEACVTLGMLTPSQAGRLAEAGLTSYNHNLDTGPDFYEDI
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCHHHHHHHHHCCCCCCCCHHHHHHH
ISTRTYDERLQTLANVREAGIGVCCGGIVGMGEGVTDRAAMLQVLANHAPHPESVPINAL
HHCCCHHHHHHHHHHHHHHCCCEECCCHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEE
VAVPGTPLEDQPAVDPFDLVRMCATARIVMPRSRVRLSAGRKSLTREAQVLCFLAGANSI
EECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEECCCCE
FYGERLLTTANNGQDDDAALLADLGIRVGEPVRAAAE
EECCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA