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The map label for this gene is entA [C]

Identifier: 170751276

GI number: 170751276

Start: 5223020

End: 5223949

Strand: Reverse

Name: entA [C]

Synonym: Mrad2831_4893

Alternate gene names: 170751276

Gene position: 5223949-5223020 (Counterclockwise)

Preceding gene: 170751277

Following gene: 170751275

Centisome position: 85.95

GC content: 76.02

Gene sequence:

>930_bases
ATGACCCGGACCCGGTGGACGACCCGCGACATGCCGAGTCAGGCCGGACGGCACGCGATCGTCACCGGCGCGACCAGCGG
GCTCGGCTACGAGGCCGCCCTCGCCCTCGCGGGGGCGGGCGCCGCGGTCACCCTCGCCGCCCGCGACGGCGAGAAGGCCC
AGCGCGCCATGGCGTCGATCCGGCACCGTCACCCGGAAGCGCGGCTGGCCTTCCGCCGCCTCGACACCGCCAGCCTCGCC
TCCGTGCGCGCCTTCGGCGAGGCCTGCCGCGCGGACGGGCAGCCCGTCGACATCCTGCTGCTCAACGCCGGCATCGCCTC
CGTGCCGCGGCGCGAGGAGACGGGGGACGGGTTCGAGCGCCAGTTCGGCACCAACTATCTCGGCCATTTCGCGCTGACCG
GGCTGCTCCTGCCCCTGGTGCCCGCGCGGGTGACCTCCCGGATCGTCCCGGTGGCGAGCCTCGCCCACCGGCCCGGACGG
ATCCACTTCGACGACCTCCAGCTCCGGCGCGCCTACGGGCCGCAGAAGGCCTACCGGCAGTCGAAGCTCGCGATGCTGAT
GTTCGGGCTCGAACTCGACCGCCGCCTGAGGGCGGCGGGGGCGCCGATCCGCGCGATCCCGGTCCATCCCGGCGCCGCCC
GGACGGACGTGTTCCGCCGCGGCGACCGGGCAGGCCCCGTCCAGCGCCTTGCCGGGCACCTGATCTTCGCGGTGATCGGC
CAGCCCGCGGCGCGGGGCGCGCTGCCGCTGCTCTTCGCCGCCACCGCGCAGGAGGCCGAGGGCGGCGCCTATTACGGTCC
CGACGGGATCTGGGAGCTGCACGGCCATCCGGCCCGGGCCGACATCGCCGCGCAGGCCCGCGACACCGCCGCCGCCGCGC
GGCTCTGGTCGGTATCCGAGGCCCTCACGGGCGTCGACTGCACCGTCTGA

Upstream 100 bases:

>100_bases
ACGCGATCGAGACGATCCGGCCCGCCGACGCCTGAGGGCCAATCCGGCCGCGAACAACCCCGCGCCCGGCGGATTAGCCG
AGGAGCGGAGAGGCGCGACG

Downstream 100 bases:

>100_bases
TCCTGCGCCCCCGACGGGCGCCGCTTGCCCCCCGACCCCGCTCTCCATAGATCCCGACGATGACGGCACTCCCCTTCGAC
AACAGCTACGCCCGCCTGCC

Product: short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTRTRWTTRDMPSQAGRHAIVTGATSGLGYEAALALAGAGAAVTLAARDGEKAQRAMASIRHRHPEARLAFRRLDTASLA
SVRAFGEACRADGQPVDILLLNAGIASVPRREETGDGFERQFGTNYLGHFALTGLLLPLVPARVTSRIVPVASLAHRPGR
IHFDDLQLRRAYGPQKAYRQSKLAMLMFGLELDRRLRAAGAPIRAIPVHPGAARTDVFRRGDRAGPVQRLAGHLIFAVIG
QPAARGALPLLFAATAQEAEGGAYYGPDGIWELHGHPARADIAAQARDTAAAARLWSVSEALTGVDCTV

Sequences:

>Translated_309_residues
MTRTRWTTRDMPSQAGRHAIVTGATSGLGYEAALALAGAGAAVTLAARDGEKAQRAMASIRHRHPEARLAFRRLDTASLA
SVRAFGEACRADGQPVDILLLNAGIASVPRREETGDGFERQFGTNYLGHFALTGLLLPLVPARVTSRIVPVASLAHRPGR
IHFDDLQLRRAYGPQKAYRQSKLAMLMFGLELDRRLRAAGAPIRAIPVHPGAARTDVFRRGDRAGPVQRLAGHLIFAVIG
QPAARGALPLLFAATAQEAEGGAYYGPDGIWELHGHPARADIAAQARDTAAAARLWSVSEALTGVDCTV
>Mature_308_residues
TRTRWTTRDMPSQAGRHAIVTGATSGLGYEAALALAGAGAAVTLAARDGEKAQRAMASIRHRHPEARLAFRRLDTASLAS
VRAFGEACRADGQPVDILLLNAGIASVPRREETGDGFERQFGTNYLGHFALTGLLLPLVPARVTSRIVPVASLAHRPGRI
HFDDLQLRRAYGPQKAYRQSKLAMLMFGLELDRRLRAAGAPIRAIPVHPGAARTDVFRRGDRAGPVQRLAGHLIFAVIGQ
PAARGALPLLFAATAQEAEGGAYYGPDGIWELHGHPARADIAAQARDTAAAARLWSVSEALTGVDCTV

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=302, Percent_Identity=39.7350993377483, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI193804850, Length=307, Percent_Identity=35.8306188925081, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI166795268, Length=292, Percent_Identity=36.986301369863, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI186928839, Length=292, Percent_Identity=36.3013698630137, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI10190746, Length=305, Percent_Identity=35.4098360655738, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI19923931, Length=260, Percent_Identity=38.4615384615385, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI7706523, Length=301, Percent_Identity=31.5614617940199, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI146231950, Length=294, Percent_Identity=37.7551020408163, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17558006, Length=211, Percent_Identity=34.5971563981043, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71992402, Length=311, Percent_Identity=29.903536977492, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193207620, Length=304, Percent_Identity=30.2631578947368, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17532805, Length=300, Percent_Identity=28.3333333333333, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17532791, Length=216, Percent_Identity=31.9444444444444, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535059, Length=300, Percent_Identity=30, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32565532, Length=135, Percent_Identity=34.8148148148148, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17505416, Length=273, Percent_Identity=27.8388278388278, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72001364, Length=303, Percent_Identity=29.3729372937294, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533174, Length=289, Percent_Identity=25.9515570934256, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533172, Length=289, Percent_Identity=25.9515570934256, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324820, Length=322, Percent_Identity=26.7080745341615, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28573296, Length=308, Percent_Identity=36.6883116883117, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI22024069, Length=303, Percent_Identity=34.6534653465347, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45554248, Length=289, Percent_Identity=38.4083044982699, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640117, Length=289, Percent_Identity=38.4083044982699, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19921754, Length=298, Percent_Identity=34.5637583892617, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24586328, Length=297, Percent_Identity=35.016835016835, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161076371, Length=261, Percent_Identity=37.1647509578544, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24656154, Length=291, Percent_Identity=33.6769759450172, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19922628, Length=291, Percent_Identity=33.6769759450172, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20129347, Length=300, Percent_Identity=30, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647946, Length=205, Percent_Identity=32.6829268292683, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45553409, Length=205, Percent_Identity=32.6829268292683, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24647948, Length=205, Percent_Identity=32.6829268292683, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24647931, Length=216, Percent_Identity=34.2592592592593, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 32966; Mature: 32835

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTRWTTRDMPSQAGRHAIVTGATSGLGYEAALALAGAGAAVTLAARDGEKAQRAMASI
CCCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
RHRHPEARLAFRRLDTASLASVRAFGEACRADGQPVDILLLNAGIASVPRREETGDGFER
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHH
QFGTNYLGHFALTGLLLPLVPARVTSRIVPVASLAHRPGRIHFDDLQLRRAYGPQKAYRQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHH
SKLAMLMFGLELDRRLRAAGAPIRAIPVHPGAARTDVFRRGDRAGPVQRLAGHLIFAVIG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHC
QPAARGALPLLFAATAQEAEGGAYYGPDGIWELHGHPARADIAAQARDTAAAARLWSVSE
CCCCCCCCCEEEEHHHHHCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ALTGVDCTV
HHCCCCCCC
>Mature Secondary Structure 
TRTRWTTRDMPSQAGRHAIVTGATSGLGYEAALALAGAGAAVTLAARDGEKAQRAMASI
CCCCCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
RHRHPEARLAFRRLDTASLASVRAFGEACRADGQPVDILLLNAGIASVPRREETGDGFER
HHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHH
QFGTNYLGHFALTGLLLPLVPARVTSRIVPVASLAHRPGRIHFDDLQLRRAYGPQKAYRQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHH
SKLAMLMFGLELDRRLRAAGAPIRAIPVHPGAARTDVFRRGDRAGPVQRLAGHLIFAVIG
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHC
QPAARGALPLLFAATAQEAEGGAYYGPDGIWELHGHPARADIAAQARDTAAAARLWSVSE
CCCCCCCCCEEEEHHHHHCCCCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ALTGVDCTV
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]