Click here to switch to the map view.

The map label for this gene is mcl2 [H]

Identifier: 170748705

GI number: 170748705

Start: 2416366

End: 2417247

Strand: Reverse

Name: mcl2 [H]

Synonym: Mrad2831_2287

Alternate gene names: 170748705

Gene position: 2417247-2416366 (Counterclockwise)

Preceding gene: 170748708

Following gene: 170748704

Centisome position: 39.77

GC content: 75.06

Gene sequence:

>882_bases
ATGAGCGAGATCCGTCCCCGTCGCAGCGTGCTGGCGATGCCGGGCTCCAACGCCCGGGCGCTGGAGAAGGCGCGGTCTCT
CCCCGCCGACGTGATCCTGATCGACCTCGAGGACGGCACCGCCCCCGCCGCGAAGGCGGCGGCCCGCGCCCAGGTCGCCG
CCGCGGTCGCCGCGCGGGGCTTCGGCCACCGCGAGGTCGTGGTCCGCATCAACCCACCCGAGACCGAGGACGGCGAGGCC
GACCTCGTCGCGCTCGCGCCGGCCGCCCCGGACGCGATCCTGGTGCCGAAGGTCCGCACCTCCGACGCGCTGATCGCCGT
CGGCTCCCGCCTGCGCCGCCTCGGCGCGCCGCTGGCGACGCGGGTCTGGGCGATGATCGAGACGCCGATGGCGGTGGTGA
ACGTCGCCGAGATCGCGGGCGCGGCCCGGGACGTGGACGGCCGGCTCGGCGCATTGGTGATCGGCCCGAACGACCTCCTG
AAGGCGTCCCGGATCCGGCCACCCGGGCGGGCCGCGCTGATGCCCTGGCTGATGAACGTCCTGGCCGCCGCCCGGGCCTA
CGAGATCGAGGCGATCGACGGCGTCTTCACCGACCTCACCGACGCGGCCGGGTTCGAGGCCGAGTGCGTCCAGGGCCGCG
ACCTCGGCTTCGACGGCAAGATGCTGATCCACCCGAGCCAGATCGGCCCGGCCAACGCGGCCTTCGGGCCGGATGCCGGG
GAGATCGCGCAGGCGCGCCGGCTGATGGACGTCTTCGACACGCCGGAGAACCGCGGGCGCGGGGTCGTCGCGGTGGACGG
CAAGATGGTCGAGCGGCTCCACGTCGAGGCGGCCCGGCGGACACTCGCCATGGCCGAGGCGATCGCGCGGCTCTCCGCGT
GA

Upstream 100 bases:

>100_bases
CGCCGTCAGCGCGGCCGGCGTTCTTGCCGGCGTTCTTGCCGGGATGCGCGCGGAAAGCTATCCGCGTCGCCGAGGGCGCC
GCCGGCCCGGGAGAAGCGTG

Downstream 100 bases:

>100_bases
GCGACCTGCCCGAGGTCGGCCTGCGTCTGCTCGACCCGCGCCTGCCGGGCTGGGGCTTCCCGCGCTGGGGCTCGTCGGCC
GCCGCCGGCCTCGACCTGCA

Product: citrate (pro-3S)-lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSEIRPRRSVLAMPGSNARALEKARSLPADVILIDLEDGTAPAAKAAARAQVAAAVAARGFGHREVVVRINPPETEDGEA
DLVALAPAAPDAILVPKVRTSDALIAVGSRLRRLGAPLATRVWAMIETPMAVVNVAEIAGAARDVDGRLGALVIGPNDLL
KASRIRPPGRAALMPWLMNVLAAARAYEIEAIDGVFTDLTDAAGFEAECVQGRDLGFDGKMLIHPSQIGPANAAFGPDAG
EIAQARRLMDVFDTPENRGRGVVAVDGKMVERLHVEAARRTLAMAEAIARLSA

Sequences:

>Translated_293_residues
MSEIRPRRSVLAMPGSNARALEKARSLPADVILIDLEDGTAPAAKAAARAQVAAAVAARGFGHREVVVRINPPETEDGEA
DLVALAPAAPDAILVPKVRTSDALIAVGSRLRRLGAPLATRVWAMIETPMAVVNVAEIAGAARDVDGRLGALVIGPNDLL
KASRIRPPGRAALMPWLMNVLAAARAYEIEAIDGVFTDLTDAAGFEAECVQGRDLGFDGKMLIHPSQIGPANAAFGPDAG
EIAQARRLMDVFDTPENRGRGVVAVDGKMVERLHVEAARRTLAMAEAIARLSA
>Mature_292_residues
SEIRPRRSVLAMPGSNARALEKARSLPADVILIDLEDGTAPAAKAAARAQVAAAVAARGFGHREVVVRINPPETEDGEAD
LVALAPAAPDAILVPKVRTSDALIAVGSRLRRLGAPLATRVWAMIETPMAVVNVAEIAGAARDVDGRLGALVIGPNDLLK
ASRIRPPGRAALMPWLMNVLAAARAYEIEAIDGVFTDLTDAAGFEAECVQGRDLGFDGKMLIHPSQIGPANAAFGPDAGE
IAQARRLMDVFDTPENRGRGVVAVDGKMVERLHVEAARRTLAMAEAIARLSA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=297, Percent_Identity=27.6094276094276, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI87081764, Length=290, Percent_Identity=33.1034482758621, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17557458, Length=285, Percent_Identity=28.7719298245614, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30759; Mature: 30628

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIRPRRSVLAMPGSNARALEKARSLPADVILIDLEDGTAPAAKAAARAQVAAAVAARG
CCCCCCCCCEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
FGHREVVVRINPPETEDGEADLVALAPAAPDAILVPKVRTSDALIAVGSRLRRLGAPLAT
CCCEEEEEEECCCCCCCCCCCEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHH
RVWAMIETPMAVVNVAEIAGAARDVDGRLGALVIGPNDLLKASRIRPPGRAALMPWLMNV
HHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHH
LAAARAYEIEAIDGVFTDLTDAAGFEAECVQGRDLGFDGKMLIHPSQIGPANAAFGPDAG
HHHHHHHEEHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHH
EIAQARRLMDVFDTPENRGRGVVAVDGKMVERLHVEAARRTLAMAEAIARLSA
HHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEIRPRRSVLAMPGSNARALEKARSLPADVILIDLEDGTAPAAKAAARAQVAAAVAARG
CCCCCCCCEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
FGHREVVVRINPPETEDGEADLVALAPAAPDAILVPKVRTSDALIAVGSRLRRLGAPLAT
CCCEEEEEEECCCCCCCCCCCEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHH
RVWAMIETPMAVVNVAEIAGAARDVDGRLGALVIGPNDLLKASRIRPPGRAALMPWLMNV
HHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHH
LAAARAYEIEAIDGVFTDLTDAAGFEAECVQGRDLGFDGKMLIHPSQIGPANAAFGPDAG
HHHHHHHEEHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHH
EIAQARRLMDVFDTPENRGRGVVAVDGKMVERLHVEAARRTLAMAEAIARLSA
HHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA