Click here to switch to the map view.

The map label for this gene is dut [H]

Identifier: 170748704

GI number: 170748704

Start: 2415899

End: 2416369

Strand: Reverse

Name: dut [H]

Synonym: Mrad2831_2286

Alternate gene names: 170748704

Gene position: 2416369-2415899 (Counterclockwise)

Preceding gene: 170748705

Following gene: 170748702

Centisome position: 39.76

GC content: 76.43

Gene sequence:

>471_bases
GTGAGCGACCTGCCCGAGGTCGGCCTGCGTCTGCTCGACCCGCGCCTGCCGGGCTGGGGCTTCCCGCGCTGGGGCTCGTC
GGCCGCCGCCGGCCTCGACCTGCATGCCTGCCTCGACGCGCCCGTGGTCCTGCCGCCTCAGGGCAAGCCGGTCCTGATCC
CCGCCGGCTTCACCGTCCTGATCCGCGACCCCGCCTGGTGCGGGCTGATCTTCCCCCGCTCCGGCCGCGGCCACCGCGAG
GGCCTCGTGCTCGGCAACGGCACCGGCGTGATCGACGCGGATTACGAGGGCCCGCTGATGGTCTCCGCCTGGAACCGCGA
CGCGTCGGAGCCCGTCCGGATCGAGCCCGGGGATCGGATCGCGCAGCTCGTCTTCACGCGGGTCACCCGGCCGGCGCTGA
CCGTGCTGGAGGGGGCGCCCGGCCCCGGATCGGAGCGGGGCGCGGGCGGCTTCGGCTCGAGCGGGCGCTAG

Upstream 100 bases:

>100_bases
GCGGGGTCGTCGCGGTGGACGGCAAGATGGTCGAGCGGCTCCACGTCGAGGCGGCCCGGCGGACACTCGCCATGGCCGAG
GCGATCGCGCGGCTCTCCGC

Downstream 100 bases:

>100_bases
GCCCCCGTCAGTCCAGCGTCTTGCCGAGCCGCCCGGCCAGGTCCTGGATGAACTGGAAGGCGGTCCGGCCCGAGCGCGAG
CCGCGGGTCGTGGCCCATTC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSDLPEVGLRLLDPRLPGWGFPRWGSSAAAGLDLHACLDAPVVLPPQGKPVLIPAGFTVLIRDPAWCGLIFPRSGRGHRE
GLVLGNGTGVIDADYEGPLMVSAWNRDASEPVRIEPGDRIAQLVFTRVTRPALTVLEGAPGPGSERGAGGFGSSGR

Sequences:

>Translated_156_residues
MSDLPEVGLRLLDPRLPGWGFPRWGSSAAAGLDLHACLDAPVVLPPQGKPVLIPAGFTVLIRDPAWCGLIFPRSGRGHRE
GLVLGNGTGVIDADYEGPLMVSAWNRDASEPVRIEPGDRIAQLVFTRVTRPALTVLEGAPGPGSERGAGGFGSSGR
>Mature_155_residues
SDLPEVGLRLLDPRLPGWGFPRWGSSAAAGLDLHACLDAPVVLPPQGKPVLIPAGFTVLIRDPAWCGLIFPRSGRGHREG
LVLGNGTGVIDADYEGPLMVSAWNRDASEPVRIEPGDRIAQLVFTRVTRPALTVLEGAPGPGSERGAGGFGSSGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=136, Percent_Identity=36.7647058823529, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI4503423, Length=151, Percent_Identity=35.7615894039735, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI70906441, Length=136, Percent_Identity=36.7647058823529, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790071, Length=154, Percent_Identity=42.8571428571429, Blast_Score=133, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71988561, Length=135, Percent_Identity=35.5555555555556, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319729, Length=143, Percent_Identity=35.6643356643357, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921126, Length=134, Percent_Identity=35.0746268656716, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583610, Length=134, Percent_Identity=35.0746268656716, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16354; Mature: 16223

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLPEVGLRLLDPRLPGWGFPRWGSSAAAGLDLHACLDAPVVLPPQGKPVLIPAGFTVL
CCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEHHCCCCEEECCCCCEEEEECCEEEE
IRDPAWCGLIFPRSGRGHREGLVLGNGTGVIDADYEGPLMVSAWNRDASEPVRIEPGDRI
EECCCEEEEEECCCCCCCCCEEEEECCCEEEECCCCCCEEEEECCCCCCCCEEECCCHHH
AQLVFTRVTRPALTVLEGAPGPGSERGAGGFGSSGR
HHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDLPEVGLRLLDPRLPGWGFPRWGSSAAAGLDLHACLDAPVVLPPQGKPVLIPAGFTVL
CCCHHHHHEEECCCCCCCCCCCCCCCCCCCCEEEHHCCCCEEECCCCCEEEEECCEEEE
IRDPAWCGLIFPRSGRGHREGLVLGNGTGVIDADYEGPLMVSAWNRDASEPVRIEPGDRI
EECCCEEEEEECCCCCCCCCEEEEECCCEEEECCCCCCEEEEECCCCCCCCEEECCCHHH
AQLVFTRVTRPALTVLEGAPGPGSERGAGGFGSSGR
HHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA