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The map label for this gene is glnP [H]

Identifier: 170748628

GI number: 170748628

Start: 2348007

End: 2348702

Strand: Reverse

Name: glnP [H]

Synonym: Mrad2831_2210

Alternate gene names: 170748628

Gene position: 2348702-2348007 (Counterclockwise)

Preceding gene: 170748629

Following gene: 170748627

Centisome position: 38.64

GC content: 69.68

Gene sequence:

>696_bases
ATGAACCCCTCGGTCGTCATCGACAATCTCGGCTACCTGCTGGTCGGGGCTTGGCCCGACGGACCGGTCGGCGGGCTTGC
CCTGACCCTGATTCTCTCCGGCCTGTCCGGTCTCGCCTCGGCCGCACTCGGGCTCATCTTCGGCGTCGTCCTGGCCGTGA
GTGCGGGTGCCGCCCGGACGATCCTCACCGCCGGGCTCGGCTTCCTCCGCGCCGTGCCGGTCGTGATGCTGATCTTCTGG
ACCTACTTCCTGCTGCCGATCCTGTTCGGCGTGTCGATCCCGGAGGCCGGTACGGTCGCGGCGGCGCTGTCGCTCGTCGG
AGGCGCCTACCTGTCCTACGGCGTGGCGTCGGGCATCGCGAGCGTCGGCCGCGGCCAGTGGGAGGCCGGCCTGGCGCTCG
GCCTCGGTCCCTGGCGCACCCTGCGATTCGTCATCCTGCCGCAGGCGCTGCGCGCCATGGCGCCGTCCTTCGTCAACCAG
TGGACCACGCTCATCAAGGACACGTCGCTCGCCTACGTGATCGGCGTGCCCGAATTGTCCTTCGTGGCCGCGCAGGTGAA
CAACCGCGCCATGATCTACCCGGCCGAGATCTTCCTCTTCGTCGGTGCCGTCTACGGCGTCCTGTGCGTCTCCCTGGACG
GGCTGGCCTCCGGTGTGAGGAGGCCCGGCAAGGTCCCGGCGCATCGCGCCAAGTGA

Upstream 100 bases:

>100_bases
CCTTCGGCATCGCGACGCTCCTCTATGTCGGCGCGGCGGCCGCGGTGGAGACGGCCGGGCAGATCCTCGGCCGCCGGTTC
CGTCCCCCGGGCCGGCACCG

Downstream 100 bases:

>100_bases
CGGAGCGGCCGAGGGGCCCACGCGCCCGAGCCGGCCCGCGGGACGTGGCCGATTCCTCCGGGCACACGCGCAAGGCGTAC
CGGATCGGGGCAGGCGCGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNPSVVIDNLGYLLVGAWPDGPVGGLALTLILSGLSGLASAALGLIFGVVLAVSAGAARTILTAGLGFLRAVPVVMLIFW
TYFLLPILFGVSIPEAGTVAAALSLVGGAYLSYGVASGIASVGRGQWEAGLALGLGPWRTLRFVILPQALRAMAPSFVNQ
WTTLIKDTSLAYVIGVPELSFVAAQVNNRAMIYPAEIFLFVGAVYGVLCVSLDGLASGVRRPGKVPAHRAK

Sequences:

>Translated_231_residues
MNPSVVIDNLGYLLVGAWPDGPVGGLALTLILSGLSGLASAALGLIFGVVLAVSAGAARTILTAGLGFLRAVPVVMLIFW
TYFLLPILFGVSIPEAGTVAAALSLVGGAYLSYGVASGIASVGRGQWEAGLALGLGPWRTLRFVILPQALRAMAPSFVNQ
WTTLIKDTSLAYVIGVPELSFVAAQVNNRAMIYPAEIFLFVGAVYGVLCVSLDGLASGVRRPGKVPAHRAK
>Mature_231_residues
MNPSVVIDNLGYLLVGAWPDGPVGGLALTLILSGLSGLASAALGLIFGVVLAVSAGAARTILTAGLGFLRAVPVVMLIFW
TYFLLPILFGVSIPEAGTVAAALSLVGGAYLSYGVASGIASVGRGQWEAGLALGLGPWRTLRFVILPQALRAMAPSFVNQ
WTTLIKDTSLAYVIGVPELSFVAAQVNNRAMIYPAEIFLFVGAVYGVLCVSLDGLASGVRRPGKVPAHRAK

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=230, Percent_Identity=32.1739130434783, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1788226, Length=218, Percent_Identity=32.1100917431193, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787030, Length=222, Percent_Identity=34.6846846846847, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87082239, Length=214, Percent_Identity=31.7757009345794, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI48994930, Length=101, Percent_Identity=40.5940594059406, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787087, Length=192, Percent_Identity=31.25, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 23851; Mature: 23851

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSVVIDNLGYLLVGAWPDGPVGGLALTLILSGLSGLASAALGLIFGVVLAVSAGAART
CCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILTAGLGFLRAVPVVMLIFWTYFLLPILFGVSIPEAGTVAAALSLVGGAYLSYGVASGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVGRGQWEAGLALGLGPWRTLRFVILPQALRAMAPSFVNQWTTLIKDTSLAYVIGVPELS
HHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHH
FVAAQVNNRAMIYPAEIFLFVGAVYGVLCVSLDGLASGVRRPGKVPAHRAK
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNPSVVIDNLGYLLVGAWPDGPVGGLALTLILSGLSGLASAALGLIFGVVLAVSAGAART
CCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILTAGLGFLRAVPVVMLIFWTYFLLPILFGVSIPEAGTVAAALSLVGGAYLSYGVASGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVGRGQWEAGLALGLGPWRTLRFVILPQALRAMAPSFVNQWTTLIKDTSLAYVIGVPELS
HHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHH
FVAAQVNNRAMIYPAEIFLFVGAVYGVLCVSLDGLASGVRRPGKVPAHRAK
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]