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The map label for this gene is dus [H]

Identifier: 170748588

GI number: 170748588

Start: 2311962

End: 2312987

Strand: Reverse

Name: dus [H]

Synonym: Mrad2831_2170

Alternate gene names: 170748588

Gene position: 2312987-2311962 (Counterclockwise)

Preceding gene: 170748590

Following gene: 170748587

Centisome position: 38.06

GC content: 74.07

Gene sequence:

>1026_bases
ATGATGACTGACCATTATTTGAGCGCGCTGCGCCGCGAGGATGCGAAGGCTGAAGCGGCAGGCACCGCCCCGCTCTGCCT
GCTCGCCCCCCTTTCCGGCGTCACCGACCTTCACATGCGCCGCATTGCCCGCCGCTGCGGTGCTGACGCGGTCATTTCCG
AGATGGTGGCGGCCCGGGAATTCACCCGCGGATCCGGCGAGGCCACACTGCGCGCCGAGGGTTCCGGCGTCGACCTGCAC
GTTGTCCAGCTCGCGGGCTGCGAGGCGGGCCCAATGGCGGAGGCCGCGCGGCTCGCGGAGGCCGGGGGCGCCGACGTGAT
CGACGTGAACATGGGCTGCCCGGCCAAGATCGTGACGGGGATCGCCTCGGGCTCGGCGCTCATGCGCGACCTCGACCACG
CCGAGCGCCTGCTCGCGTCCGTGCGGGGCGCCGTGTCGATCCCGGTGACCGTCAAGATGCGCCTCGGCTGGGACGACGCG
AGCCGCAACGCCGCGGATCTCGCCGCCCGCGCCGAGCGGCTGGGCCTGAACGCCGTGACGGTCCACGGGCGGACGCGGCA
GCAATTCTACAAAGGACGCGCCGACTGGGCGGCGATCCGCGCCGTGGTCGCGGCGGTGTCGATCCCGGTGATCGCCAACG
GTGACGTGACCGACTTCGCCAGCGCCCGCGCTTGCCTGGACGCCTCGGGCGCCGCCGGGGTCATGATCGGCCGCGCGGCG
ACCGGCCGCCCCTGGCTGGTCGGGCAGGTCGCCGCCGCGCTCACGGGCCGGCCGGCCGCGGAGCCGTCGCGGTCCGAACA
GGCCGCCCTGGCCGCGGAGCACTATGCGGGGCTGCTCTCCCTCTACGGCCGCGACATGGGCGTGCGCCACGCGCGCAAGC
ACCTCGCCGCCTATGCCGAGACCGGCGGCGCGCTCGATCCGGCCGAACGGTCGGCCCTCCTGACCACCACCGATCCGGAG
ACGGCGCTCGCCTTGCTGGCCCGCGCCTTCGCGGATGCGGCCGATCCCGTCCAGGAGGCCGCGTGA

Upstream 100 bases:

>100_bases
CCTGCCGCGGAGGGTGCCCGTCGTTTAAGCGGGGGAGGGCCCGAAATCAACGCGCGGTCCCGCTTGCCAGCGGACCGGCC
TTGCCTCATTGTTGTGCAGA

Downstream 100 bases:

>100_bases
GCGACGAGCCCCTCCCGAAAGAGTCCGGCAACCGGCGCGACACCGCCGCCGAGGCCGTGCTCAACGCCCTGCCGCTGCCG
GTACTCACCATCGGCGCCGA

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MMTDHYLSALRREDAKAEAAGTAPLCLLAPLSGVTDLHMRRIARRCGADAVISEMVAAREFTRGSGEATLRAEGSGVDLH
VVQLAGCEAGPMAEAARLAEAGGADVIDVNMGCPAKIVTGIASGSALMRDLDHAERLLASVRGAVSIPVTVKMRLGWDDA
SRNAADLAARAERLGLNAVTVHGRTRQQFYKGRADWAAIRAVVAAVSIPVIANGDVTDFASARACLDASGAAGVMIGRAA
TGRPWLVGQVAAALTGRPAAEPSRSEQAALAAEHYAGLLSLYGRDMGVRHARKHLAAYAETGGALDPAERSALLTTTDPE
TALALLARAFADAADPVQEAA

Sequences:

>Translated_341_residues
MMTDHYLSALRREDAKAEAAGTAPLCLLAPLSGVTDLHMRRIARRCGADAVISEMVAAREFTRGSGEATLRAEGSGVDLH
VVQLAGCEAGPMAEAARLAEAGGADVIDVNMGCPAKIVTGIASGSALMRDLDHAERLLASVRGAVSIPVTVKMRLGWDDA
SRNAADLAARAERLGLNAVTVHGRTRQQFYKGRADWAAIRAVVAAVSIPVIANGDVTDFASARACLDASGAAGVMIGRAA
TGRPWLVGQVAAALTGRPAAEPSRSEQAALAAEHYAGLLSLYGRDMGVRHARKHLAAYAETGGALDPAERSALLTTTDPE
TALALLARAFADAADPVQEAA
>Mature_341_residues
MMTDHYLSALRREDAKAEAAGTAPLCLLAPLSGVTDLHMRRIARRCGADAVISEMVAAREFTRGSGEATLRAEGSGVDLH
VVQLAGCEAGPMAEAARLAEAGGADVIDVNMGCPAKIVTGIASGSALMRDLDHAERLLASVRGAVSIPVTVKMRLGWDDA
SRNAADLAARAERLGLNAVTVHGRTRQQFYKGRADWAAIRAVVAAVSIPVIANGDVTDFASARACLDASGAAGVMIGRAA
TGRPWLVGQVAAALTGRPAAEPSRSEQAALAAEHYAGLLSLYGRDMGVRHARKHLAAYAETGGALDPAERSALLTTTDPE
TALALLARAFADAADPVQEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=309, Percent_Identity=29.4498381877023, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI239788462, Length=275, Percent_Identity=30.9090909090909, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI40807366, Length=219, Percent_Identity=32.8767123287671, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI31742496, Length=232, Percent_Identity=28.8793103448276, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=31.5573770491803, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789660, Length=287, Percent_Identity=41.1149825783972, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=36.5217391304348, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=26.3492063492064, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=280, Percent_Identity=28.9285714285714, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=245, Percent_Identity=29.7959183673469, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=239, Percent_Identity=29.7071129707113, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=271, Percent_Identity=28.4132841328413, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=326, Percent_Identity=26.3803680981595, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=30.1652892561983, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=25.2212389380531, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585320, Length=281, Percent_Identity=33.8078291814947, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24580595, Length=235, Percent_Identity=28.0851063829787, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920448, Length=235, Percent_Identity=28.0851063829787, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=28.7610619469027, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45549423, Length=234, Percent_Identity=29.0598290598291, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35234; Mature: 35234

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDHYLSALRREDAKAEAAGTAPLCLLAPLSGVTDLHMRRIARRCGADAVISEMVAARE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
FTRGSGEATLRAEGSGVDLHVVQLAGCEAGPMAEAARLAEAGGADVIDVNMGCPAKIVTG
HCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHH
IASGSALMRDLDHAERLLASVRGAVSIPVTVKMRLGWDDASRNAADLAARAERLGLNAVT
HCCCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEE
VHGRTRQQFYKGRADWAAIRAVVAAVSIPVIANGDVTDFASARACLDASGAAGVMIGRAA
ECCCHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCCCCEEEECCC
TGRPWLVGQVAAALTGRPAAEPSRSEQAALAAEHYAGLLSLYGRDMGVRHARKHLAAYAE
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TGGALDPAERSALLTTTDPETALALLARAFADAADPVQEAA
CCCCCCCHHCCEEEEECCHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MMTDHYLSALRREDAKAEAAGTAPLCLLAPLSGVTDLHMRRIARRCGADAVISEMVAARE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
FTRGSGEATLRAEGSGVDLHVVQLAGCEAGPMAEAARLAEAGGADVIDVNMGCPAKIVTG
HCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHH
IASGSALMRDLDHAERLLASVRGAVSIPVTVKMRLGWDDASRNAADLAARAERLGLNAVT
HCCCHHHHHHHHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEE
VHGRTRQQFYKGRADWAAIRAVVAAVSIPVIANGDVTDFASARACLDASGAAGVMIGRAA
ECCCHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCCCCEEEECCC
TGRPWLVGQVAAALTGRPAAEPSRSEQAALAAEHYAGLLSLYGRDMGVRHARKHLAAYAE
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TGGALDPAERSALLTTTDPETALALLARAFADAADPVQEAA
CCCCCCCHHCCEEEEECCHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]