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The map label for this gene is def [C]

Identifier: 170748510

GI number: 170748510

Start: 2222587

End: 2223084

Strand: Reverse

Name: def [C]

Synonym: Mrad2831_2092

Alternate gene names: 170748510

Gene position: 2223084-2222587 (Counterclockwise)

Preceding gene: 170748511

Following gene: 170748509

Centisome position: 36.58

GC content: 73.9

Gene sequence:

>498_bases
ATGCCGGCGCGCCCGCTCGTCCTGTTTCCCGATCCGCGCCTGACGCGCCCGGCCGAGCCGGTCGACGCGTTCGGGCCATC
CCTGCAGGCCCTGGCCGACGACGTCCGGGACACGCTCATGGCCGAGAGCGCCATCGGCCTGACGGCCCCGCATATCGGCG
TCCCCGCGCGCCTCGTGGTGGTCCGGATGTCGCCGAACGAGAGCGCCCGCATCTACGTGAACCCCGTCGTGTCCTGGGCG
TCCCCCGAGACGGCAACCCACGACGAGGGCAGCGTCAGCATGCCGGGGGTGCGCGAGCGGATCGCCCGGCCCGCCCGCAT
CCGCCTGAGCTACCGCGACCTCGACGGGTCCGCCCGCGAGGCGGAGACGGAGGGCTTCCCGGCCGCGGTCCTCCAGCACG
AGATCGACCAGCTCGACGGCGTGTTCTGGATCGCCCGCCTGTCGCGGCTGAAGCGCGAGCGGCTGCTGAAGCGGTTCGAG
AAGGGCCGGCCCGCCTAG

Upstream 100 bases:

>100_bases
CGGGCCCGTCTTGACGACCCGCTCCGCGGCGCGGCTTGCATGGTGCGCGGCGGGCCCTCGCGTCGCCGCAGGTGGACCGA
CCCACGCGGACTGATCAACC

Downstream 100 bases:

>100_bases
ACAGGTCCGGGCCCCGCCGGCGGGAGCCGGCGCCCCGCCGCGAGCGTGCTGGCGGAGCCGGCGCAGGGAGGGCTAGGATG
AGGGCGACGTCGCCAGCCCT

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MPARPLVLFPDPRLTRPAEPVDAFGPSLQALADDVRDTLMAESAIGLTAPHIGVPARLVVVRMSPNESARIYVNPVVSWA
SPETATHDEGSVSMPGVRERIARPARIRLSYRDLDGSAREAETEGFPAAVLQHEIDQLDGVFWIARLSRLKRERLLKRFE
KGRPA

Sequences:

>Translated_165_residues
MPARPLVLFPDPRLTRPAEPVDAFGPSLQALADDVRDTLMAESAIGLTAPHIGVPARLVVVRMSPNESARIYVNPVVSWA
SPETATHDEGSVSMPGVRERIARPARIRLSYRDLDGSAREAETEGFPAAVLQHEIDQLDGVFWIARLSRLKRERLLKRFE
KGRPA
>Mature_164_residues
PARPLVLFPDPRLTRPAEPVDAFGPSLQALADDVRDTLMAESAIGLTAPHIGVPARLVVVRMSPNESARIYVNPVVSWAS
PETATHDEGSVSMPGVRERIARPARIRLSYRDLDGSAREAETEGFPAAVLQHEIDQLDGVFWIARLSRLKRERLLKRFEK
GRPA

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=24.6987951807229, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18254; Mature: 18123

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARPLVLFPDPRLTRPAEPVDAFGPSLQALADDVRDTLMAESAIGLTAPHIGVPARLVV
CCCCCEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
VRMSPNESARIYVNPVVSWASPETATHDEGSVSMPGVRERIARPARIRLSYRDLDGSARE
EEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCC
AETEGFPAAVLQHEIDQLDGVFWIARLSRLKRERLLKRFEKGRPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PARPLVLFPDPRLTRPAEPVDAFGPSLQALADDVRDTLMAESAIGLTAPHIGVPARLVV
CCCCEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
VRMSPNESARIYVNPVVSWASPETATHDEGSVSMPGVRERIARPARIRLSYRDLDGSARE
EEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCC
AETEGFPAAVLQHEIDQLDGVFWIARLSRLKRERLLKRFEKGRPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]