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The map label for this gene is smeIM [H]

Identifier: 170747153

GI number: 170747153

Start: 756607

End: 757713

Strand: Reverse

Name: smeIM [H]

Synonym: Mrad2831_0719

Alternate gene names: 170747153

Gene position: 757713-756607 (Counterclockwise)

Preceding gene: 170747159

Following gene: 170747151

Centisome position: 12.47

GC content: 69.83

Gene sequence:

>1107_bases
ATGGGTCTCGTACCCGCCCAGCGTTCCCTGCCCCTCGACCAGGTTCTCGTCGGGGACTGCATCGCGGCCATGAACGCCCT
GCCGGCGTCGAGCGTCGACTGCGTGTTCGCCGATCCGCCCTACAATCTCCAGCTCGGCGACGCCGGCCTCCTGCGCCCGG
ACCAGAGTCGCGTCGACGCGGTCGACGACGACTGGGACAAGTTCGCGACCTTCGAGGCCTACGACACCTTCACCCGGGAC
TGGCTCTCCGCCTGCCGCCGCGTGATGAAGCCGAACGCGACCCTCTGGGTGATCGGGTCGTACCACAACATCTTCCGGGT
CGGGAGCGCGTTGCAGGATCTCGGTTACTGGATCCTCAACGACATCGTCTGGCGCAAGGCCAACCCGATGCCGAACTTCC
GCGGCAAGCGCTTCACCAACGCCCACGAGACCCTGATCTGGGCCTCGCGCTCGGCCGACTCGAAGGGCTACACCTTCCAT
TACGAGGCGCTGAAAGGGGGCAACGAGGACCTCCAGATGCGCTCGGACTGGTTCATCCCCCTCTGCACCGGCGAGGAGCG
GCTGAAGGATGCGGAGGGCCGCAAGGTCCACCCGACCCAGAAGCCCGAGGCCCTGCTCGCCCGGACGCTGCTCGCGGCGA
CCAATCCCGGCGACGTGGTGCTCGACCCGTTCTTCGGCACCGGCACCACCGGCGCCGTCGCCAAGCGCCTCGGCCGCCGC
TTCATCGGCATCGAGCGCGAGGCGACCTACGCTGAGGCCGCCCGGGAGCGGATCGCCGCCGTCGAGACCCTGTCGAGGGC
CGCCCTCATGGTGGCGCCGACCAAGCGCGCCGAACCGCGGGTGCCGTTCCTGTCGGTGATCGAGGCGGGCCACATCCGCC
CCGGCGAGACCGTGACCGACGAGCGCCGCCGGTTCCGCGCCACGGTGCGCCCGGACGGCCAGCTCGACAACGGCCTCGTC
ATCGGCTCGATCCACAAGATCGGCGCGCTGGTGCAGGGCCTTCCGGCCTGCAACGGCTGGACCTTCTGGCACGTGGAGCG
CGGCGGCAAGCCGGTGGTGATCGACACCTTCCGGGCCGGTCTCCGCCAGGCGATGGCGAACGGCTGA

Upstream 100 bases:

>100_bases
GTAACCGGTGTTTGCCATGGCTTCCCCGCGTACCGTGGCTGCCGACGCCGTCGCCCGGAAACAGGTCTCGCGTGCCGGGC
GGCCCGCGTCGGCGCCACGG

Downstream 100 bases:

>100_bases
CGCGCCGCCGTCTCTGCGAGCGCGGCGAAGCGAGCCGGTCGCGCGACGTCTCGAGGCCTCGCGCTGCCCTGGGTCGCTTC
GCTGCGCCCGCGATGACGGT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MGLVPAQRSLPLDQVLVGDCIAAMNALPASSVDCVFADPPYNLQLGDAGLLRPDQSRVDAVDDDWDKFATFEAYDTFTRD
WLSACRRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKANPMPNFRGKRFTNAHETLIWASRSADSKGYTFH
YEALKGGNEDLQMRSDWFIPLCTGEERLKDAEGRKVHPTQKPEALLARTLLAATNPGDVVLDPFFGTGTTGAVAKRLGRR
FIGIEREATYAEAARERIAAVETLSRAALMVAPTKRAEPRVPFLSVIEAGHIRPGETVTDERRRFRATVRPDGQLDNGLV
IGSIHKIGALVQGLPACNGWTFWHVERGGKPVVIDTFRAGLRQAMANG

Sequences:

>Translated_368_residues
MGLVPAQRSLPLDQVLVGDCIAAMNALPASSVDCVFADPPYNLQLGDAGLLRPDQSRVDAVDDDWDKFATFEAYDTFTRD
WLSACRRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKANPMPNFRGKRFTNAHETLIWASRSADSKGYTFH
YEALKGGNEDLQMRSDWFIPLCTGEERLKDAEGRKVHPTQKPEALLARTLLAATNPGDVVLDPFFGTGTTGAVAKRLGRR
FIGIEREATYAEAARERIAAVETLSRAALMVAPTKRAEPRVPFLSVIEAGHIRPGETVTDERRRFRATVRPDGQLDNGLV
IGSIHKIGALVQGLPACNGWTFWHVERGGKPVVIDTFRAGLRQAMANG
>Mature_367_residues
GLVPAQRSLPLDQVLVGDCIAAMNALPASSVDCVFADPPYNLQLGDAGLLRPDQSRVDAVDDDWDKFATFEAYDTFTRDW
LSACRRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILNDIVWRKANPMPNFRGKRFTNAHETLIWASRSADSKGYTFHY
EALKGGNEDLQMRSDWFIPLCTGEERLKDAEGRKVHPTQKPEALLARTLLAATNPGDVVLDPFFGTGTTGAVAKRLGRRF
IGIEREATYAEAARERIAAVETLSRAALMVAPTKRAEPRVPFLSVIEAGHIRPGETVTDERRRFRATVRPDGQLDNGLVI
GSIHKIGALVQGLPACNGWTFWHVERGGKPVVIDTFRAGLRQAMANG

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=274, Percent_Identity=31.021897810219, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 40731; Mature: 40600

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLVPAQRSLPLDQVLVGDCIAAMNALPASSVDCVFADPPYNLQLGDAGLLRPDQSRVDA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCCHHHCCC
VDDDWDKFATFEAYDTFTRDWLSACRRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILN
CCCCHHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
DIVWRKANPMPNFRGKRFTNAHETLIWASRSADSKGYTFHYEALKGGNEDLQMRSDWFIP
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEHHHCCCCCCCEECCCEEEE
LCTGEERLKDAEGRKVHPTQKPEALLARTLLAATNPGDVVLDPFFGTGTTGAVAKRLGRR
EECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHH
FIGIEREATYAEAARERIAAVETLSRAALMVAPTKRAEPRVPFLSVIEAGHIRPGETVTD
HCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHH
ERRRFRATVRPDGQLDNGLVIGSIHKIGALVQGLPACNGWTFWHVERGGKPVVIDTFRAG
HHHHEEEECCCCCCCCCCEEEECHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEHHHHHH
LRQAMANG
HHHHHHCC
>Mature Secondary Structure 
GLVPAQRSLPLDQVLVGDCIAAMNALPASSVDCVFADPPYNLQLGDAGLLRPDQSRVDA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCCCCCCHHHCCC
VDDDWDKFATFEAYDTFTRDWLSACRRVMKPNATLWVIGSYHNIFRVGSALQDLGYWILN
CCCCHHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
DIVWRKANPMPNFRGKRFTNAHETLIWASRSADSKGYTFHYEALKGGNEDLQMRSDWFIP
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEHHHCCCCCCCEECCCEEEE
LCTGEERLKDAEGRKVHPTQKPEALLARTLLAATNPGDVVLDPFFGTGTTGAVAKRLGRR
EECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHH
FIGIEREATYAEAARERIAAVETLSRAALMVAPTKRAEPRVPFLSVIEAGHIRPGETVTD
HCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHH
ERRRFRATVRPDGQLDNGLVIGSIHKIGALVQGLPACNGWTFWHVERGGKPVVIDTFRAG
HHHHEEEECCCCCCCCCCEEEECHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEHHHHHH
LRQAMANG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]