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The map label for this gene is mdeA [H]

Identifier: 170747124

GI number: 170747124

Start: 731854

End: 733146

Strand: Reverse

Name: mdeA [H]

Synonym: Mrad2831_0690

Alternate gene names: 170747124

Gene position: 733146-731854 (Counterclockwise)

Preceding gene: 170747125

Following gene: 170747121

Centisome position: 12.06

GC content: 70.92

Gene sequence:

>1293_bases
ATGACGGACCAGAACCGACCGCACCGCTTCGCGACCCGGGCGATCCACGCCGGCGCGGCGCCGGATCCCGCCACGGGCGC
GCGGGTCCAGCCGATCTACTTCACCAACGGCTTCGTCTTCGACTCGACCGAGCAGGCCGCCGACATCTTCGCCGGCCGCC
GGACCGGCTTCTCCTACTCGCGCGGTTCCAACCCGACGGTCGCCGCGCTGGAGCGCCGGATCGCCGACCTGGAGGGCGCG
AAGGCGGGGGTGGCGGTGTCCTCCGGGCAGTCCGCCATGCAGCTGGTCCTCATGACGCTGATGAAGAGCGGCGACGCCTA
TGTGGCATCCCCGCGTCTGTTCGGCGGCTCGCTCGGCCTGATGCGCCGCCTCGACGGGCGCTACGACTTGAAGCCGCGCT
TCGCGCGCGGCATGGCCCCCGAGGACTTCGAGGCGGCCATCACGCCGGACTGCCGGGCCATCTTCTGCGAATCGATCGTG
AATCCCTGCGCGTCGGTGGTGGACCTCGACGGGATCGCCGCGGTGGCCCGCCGCCACAATCTGCCGCTCGTCGTGGACAA
CACCCTGGCCTCGCCGGCCCTGATCCGGCCGATCGAGCACGGCGCCGACATCGTCTGGCACTCCACCTCCAAGTTCCTGA
GCGGCTCCGGCCAGACCATCGGCGGCGTGATCTGCGACGCCGGCCGCTTCGACTGGGCGAAGGCCGGCGGCTACAACCTG
ATCACCGAGCCTTGGCCGGACTACGACGGGCTGGTGCTCACCGAGGCCTTCCCGGCGACCCCCTTCGCGGCGGCGTGCCG
GTTCTTCGGCCTGCGGGACCTCGGGCCCGGCCTGTCGCCGATGAACGCGTTCCTGACGCTCATGGGGATCGAGACCCTGC
CGCTGCGCATGGAGCGGCACTGCGCCAACGCCCGGGCGGTGGCGGCCTTCCTGAAGGATCACCCGGCTGTGGCGTGGGTG
AGCTACCCGATGCTGCCGGGCCAGCCCGGCGAGGACCTCGCCCGCCGCTATACCCCGGACGGGCCGGGCTCGATCTTCAC
CTTCGCGCTGAAGGGCGGTGAGCCGGCGGCGCTGCGGTTCATCGCCGGACTCGAGCTGATCTCGCATCTGGTGAATATCG
GCGAGATCAAGTCGCTGGCGATCCACCCGTCCTCGACCACGCATCGGCAACTGCCGGAGCACGAGAAGGCCGCCGCGCAG
GTCGGGCCGGAGACCGTGCGGCTGTCGATCGGGCTGGAGAACGAGGCCGACCTGATCGCGGATCTCAAGCAGGCGCTGGA
CCGCCTCCCCTGA

Upstream 100 bases:

>100_bases
CTGGTCCGGACCCTGCAGGCCTTCATCCGCCGCGCGGCTTGACGCGGGGATCCGCCCGGGCTGACACCCGGTCCGGGCCC
CGAACACCCGACCGAGAGCC

Downstream 100 bases:

>100_bases
TCCGTCATCGCGCGCGCGGCGAAGCGACCCGGAGCCGCGCGATCGCGATCGCTGTGGCGCCGCTGAATCGCTCCGCTCCG
CTCGCAAGGACGGCCGCGGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTDQNRPHRFATRAIHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAGRRTGFSYSRGSNPTVAALERRIADLEGA
KAGVAVSSGQSAMQLVLMTLMKSGDAYVASPRLFGGSLGLMRRLDGRYDLKPRFARGMAPEDFEAAITPDCRAIFCESIV
NPCASVVDLDGIAAVARRHNLPLVVDNTLASPALIRPIEHGADIVWHSTSKFLSGSGQTIGGVICDAGRFDWAKAGGYNL
ITEPWPDYDGLVLTEAFPATPFAAACRFFGLRDLGPGLSPMNAFLTLMGIETLPLRMERHCANARAVAAFLKDHPAVAWV
SYPMLPGQPGEDLARRYTPDGPGSIFTFALKGGEPAALRFIAGLELISHLVNIGEIKSLAIHPSSTTHRQLPEHEKAAAQ
VGPETVRLSIGLENEADLIADLKQALDRLP

Sequences:

>Translated_430_residues
MTDQNRPHRFATRAIHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAGRRTGFSYSRGSNPTVAALERRIADLEGA
KAGVAVSSGQSAMQLVLMTLMKSGDAYVASPRLFGGSLGLMRRLDGRYDLKPRFARGMAPEDFEAAITPDCRAIFCESIV
NPCASVVDLDGIAAVARRHNLPLVVDNTLASPALIRPIEHGADIVWHSTSKFLSGSGQTIGGVICDAGRFDWAKAGGYNL
ITEPWPDYDGLVLTEAFPATPFAAACRFFGLRDLGPGLSPMNAFLTLMGIETLPLRMERHCANARAVAAFLKDHPAVAWV
SYPMLPGQPGEDLARRYTPDGPGSIFTFALKGGEPAALRFIAGLELISHLVNIGEIKSLAIHPSSTTHRQLPEHEKAAAQ
VGPETVRLSIGLENEADLIADLKQALDRLP
>Mature_429_residues
TDQNRPHRFATRAIHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAGRRTGFSYSRGSNPTVAALERRIADLEGAK
AGVAVSSGQSAMQLVLMTLMKSGDAYVASPRLFGGSLGLMRRLDGRYDLKPRFARGMAPEDFEAAITPDCRAIFCESIVN
PCASVVDLDGIAAVARRHNLPLVVDNTLASPALIRPIEHGADIVWHSTSKFLSGSGQTIGGVICDAGRFDWAKAGGYNLI
TEPWPDYDGLVLTEAFPATPFAAACRFFGLRDLGPGLSPMNAFLTLMGIETLPLRMERHCANARAVAAFLKDHPAVAWVS
YPMLPGQPGEDLARRYTPDGPGSIFTFALKGGEPAALRFIAGLELISHLVNIGEIKSLAIHPSSTTHRQLPEHEKAAAQV
GPETVRLSIGLENEADLIADLKQALDRLP

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=424, Percent_Identity=33.0188679245283, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI299473759, Length=424, Percent_Identity=31.1320754716981, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI25453487, Length=423, Percent_Identity=29.3144208037825, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1790375, Length=422, Percent_Identity=30.8056872037915, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789383, Length=434, Percent_Identity=24.1935483870968, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71980708, Length=405, Percent_Identity=30.1234567901235, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=425, Percent_Identity=31.0588235294118, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=31.2941176470588, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=38.2151029748284, Blast_Score=276, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=30.4449648711944, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=24.7747747747748, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=360, Percent_Identity=25, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922554, Length=432, Percent_Identity=30.5555555555556, Blast_Score=191, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46085; Mature: 45954

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQNRPHRFATRAIHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAGRRTGFSYS
CCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHCCCCCCCCC
RGSNPTVAALERRIADLEGAKAGVAVSSGQSAMQLVLMTLMKSGDAYVASPRLFGGSLGL
CCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
MRRLDGRYDLKPRFARGMAPEDFEAAITPDCRAIFCESIVNPCASVVDLDGIAAVARRHN
HHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPLVVDNTLASPALIRPIEHGADIVWHSTSKFLSGSGQTIGGVICDAGRFDWAKAGGYNL
CCEEECCCCCCCHHHCHHHCCCCEEEECCHHHHCCCCCEECEEEECCCCCCCHHCCCCCC
ITEPWPDYDGLVLTEAFPATPFAAACRFFGLRDLGPGLSPMNAFLTLMGIETLPLRMERH
CCCCCCCCCCEEEECCCCCCCHHHHHHHHCHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
CANARAVAAFLKDHPAVAWVSYPMLPGQPGEDLARRYTPDGPGSIFTFALKGGEPAALRF
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHH
IAGLELISHLVNIGEIKSLAIHPSSTTHRQLPEHEKAAAQVGPETVRLSIGLENEADLIA
HHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCCHHHHHHHCCCCEEEEEECCCCCHHHHH
DLKQALDRLP
HHHHHHHCCC
>Mature Secondary Structure 
TDQNRPHRFATRAIHAGAAPDPATGARVQPIYFTNGFVFDSTEQAADIFAGRRTGFSYS
CCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHCCCCCCCCC
RGSNPTVAALERRIADLEGAKAGVAVSSGQSAMQLVLMTLMKSGDAYVASPRLFGGSLGL
CCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHH
MRRLDGRYDLKPRFARGMAPEDFEAAITPDCRAIFCESIVNPCASVVDLDGIAAVARRHN
HHHCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPLVVDNTLASPALIRPIEHGADIVWHSTSKFLSGSGQTIGGVICDAGRFDWAKAGGYNL
CCEEECCCCCCCHHHCHHHCCCCEEEECCHHHHCCCCCEECEEEECCCCCCCHHCCCCCC
ITEPWPDYDGLVLTEAFPATPFAAACRFFGLRDLGPGLSPMNAFLTLMGIETLPLRMERH
CCCCCCCCCCEEEECCCCCCCHHHHHHHHCHHHCCCCCCHHHHHHHHHCCCCCCHHHHHH
CANARAVAAFLKDHPAVAWVSYPMLPGQPGEDLARRYTPDGPGSIFTFALKGGEPAALRF
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHHHHHH
IAGLELISHLVNIGEIKSLAIHPSSTTHRQLPEHEKAAAQVGPETVRLSIGLENEADLIA
HHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCCHHHHHHHCCCCEEEEEECCCCCHHHHH
DLKQALDRLP
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]