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The map label for this gene is carA [H]

Identifier: 170747004

GI number: 170747004

Start: 614394

End: 615626

Strand: Reverse

Name: carA [H]

Synonym: Mrad2831_0570

Alternate gene names: 170747004

Gene position: 615626-614394 (Counterclockwise)

Preceding gene: 170747009

Following gene: 170747003

Centisome position: 10.13

GC content: 71.05

Gene sequence:

>1233_bases
ATGCTGCAAGACGCCGCCGCGCATGCCCCCGAGCCTCAGGCTCCCGAGCCCTGGGCCGAGCCCGTCGCGACGGCTCTGCT
GGTGCTGGCCGACGGCACCATCCTGGAGGGCTTCGGCATCGGCCAGACCGGCGTGGCCGACGGGGAGGTCTGCTTCAACA
CCGCGATGACCGGCTACCAGGAGATCCTGACGGACCCGTCCTATGCCGGTCAGATCGTCACCTTCACCTTCCCGCATATC
GGCAATGTCGGCACCAACGACGAGGACCTCGAGAGCCTCGAGGCGGCGCCGGCCTCGGGCGTCCGCGGCACCGTCGTCGC
CTCGGCGGTGACGAACCCGTCGAACTGGCGGTCCTCGTCGCATCTCGACGGGTGGCTCAGGGCCCGCGGCATCGTCGGCA
TCACGGGGGTCGACACCCGGGCACTCACGGCCCGCATCCGCGACCAGGGCATGCCGAACGCGGTGATCGCCAACGATCCC
GAAGGCAAGTTCGACCGCGAGGCCCTCAAGGCCCGCGCGGCGGCCCTCGCGCCCATGGAGGGGCTCGACCTCGTGCCGCC
GGTGACCAGCCGCGCGTCGTCGGAGTGGTCCGAGACCGTCTGGGCACTCGGCAACGGTTACGGCAAGCGCGCACCCGGCG
AGGGCCTCAAGGTCGTGGCCATCGACTACGGCGTGAAGCGCAACATCCTGCGCCTCCTCGCCCAGGCCGGCTGCGACGTC
ACCGTGGTGCCGGCCACCACGACGGCCGACGAGATCCTGGCGCTGAAGCCGGACGGCGTGTTCCTGTCCAACGGTCCCGG
CGACCCGGCCGCCACCGGCGCCTACGCGGTCCCGGTGATTCGCAAGCTGCTGGACGAGAAGGTGCCGACCTTCGGCATCT
GCCTCGGCCACCAGCTCATGGGTCTGGCCCTCGGCGGCCGGACCGTGAAGATGGGCCAGGGCCATCACGGCGCGAACCAC
CCGGTGAAGGACAAGACCACCGGGAAGGTCGAGATCGTGTCGATGAACCACGGCTTCGCGGTGGACCCGGGCAGCCTCCC
GGAGACGGCGGTGGAGACGCACGTGTCGCTGTTCGACGGCTCGAATTGCGGCCTGACCCTCACCGACCGCCCGGCCTTCT
CGGTGCAGCACCACCCGGAGGCCTCCCCCGGCCCGCGCGACAGCCACTACCTGTTCGAGCGCTTCGTGACGCTGATGCGC
TCCGGCGCGCCGGAGACCGCGAAGGGCGCCTGA

Upstream 100 bases:

>100_bases
GCGGGCCGCTCGCTAAGAGCGCCGCACACGATCATGCCGCAAGCCGAGAGCGCCGAAACCCGGCGCCCGCCTTCGGCCGG
ACATAAGCGAGACTCAGCCC

Downstream 100 bases:

>100_bases
GACCCGGGCGCCGGCATCGGCCCGCCGTCATCGCTCCGCAGCGCGGTGACGGCCGGGGACAGATAAGCCGGCGCCGCGAT
CCCAATCGGGCCGCGGCGGG

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MLQDAAAHAPEPQAPEPWAEPVATALLVLADGTILEGFGIGQTGVADGEVCFNTAMTGYQEILTDPSYAGQIVTFTFPHI
GNVGTNDEDLESLEAAPASGVRGTVVASAVTNPSNWRSSSHLDGWLRARGIVGITGVDTRALTARIRDQGMPNAVIANDP
EGKFDREALKARAAALAPMEGLDLVPPVTSRASSEWSETVWALGNGYGKRAPGEGLKVVAIDYGVKRNILRLLAQAGCDV
TVVPATTTADEILALKPDGVFLSNGPGDPAATGAYAVPVIRKLLDEKVPTFGICLGHQLMGLALGGRTVKMGQGHHGANH
PVKDKTTGKVEIVSMNHGFAVDPGSLPETAVETHVSLFDGSNCGLTLTDRPAFSVQHHPEASPGPRDSHYLFERFVTLMR
SGAPETAKGA

Sequences:

>Translated_410_residues
MLQDAAAHAPEPQAPEPWAEPVATALLVLADGTILEGFGIGQTGVADGEVCFNTAMTGYQEILTDPSYAGQIVTFTFPHI
GNVGTNDEDLESLEAAPASGVRGTVVASAVTNPSNWRSSSHLDGWLRARGIVGITGVDTRALTARIRDQGMPNAVIANDP
EGKFDREALKARAAALAPMEGLDLVPPVTSRASSEWSETVWALGNGYGKRAPGEGLKVVAIDYGVKRNILRLLAQAGCDV
TVVPATTTADEILALKPDGVFLSNGPGDPAATGAYAVPVIRKLLDEKVPTFGICLGHQLMGLALGGRTVKMGQGHHGANH
PVKDKTTGKVEIVSMNHGFAVDPGSLPETAVETHVSLFDGSNCGLTLTDRPAFSVQHHPEASPGPRDSHYLFERFVTLMR
SGAPETAKGA
>Mature_410_residues
MLQDAAAHAPEPQAPEPWAEPVATALLVLADGTILEGFGIGQTGVADGEVCFNTAMTGYQEILTDPSYAGQIVTFTFPHI
GNVGTNDEDLESLEAAPASGVRGTVVASAVTNPSNWRSSSHLDGWLRARGIVGITGVDTRALTARIRDQGMPNAVIANDP
EGKFDREALKARAAALAPMEGLDLVPPVTSRASSEWSETVWALGNGYGKRAPGEGLKVVAIDYGVKRNILRLLAQAGCDV
TVVPATTTADEILALKPDGVFLSNGPGDPAATGAYAVPVIRKLLDEKVPTFGICLGHQLMGLALGGRTVKMGQGHHGANH
PVKDKTTGKVEIVSMNHGFAVDPGSLPETAVETHVSLFDGSNCGLTLTDRPAFSVQHHPEASPGPRDSHYLFERFVTLMR
SGAPETAKGA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=36.8146214099217, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI169790915, Length=394, Percent_Identity=35.7868020304569, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI21361331, Length=394, Percent_Identity=35.7868020304569, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=49.21875, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789760, Length=128, Percent_Identity=35.15625, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=388, Percent_Identity=34.2783505154639, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324878, Length=386, Percent_Identity=38.0829015544041, Blast_Score=225, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=390, Percent_Identity=36.4102564102564, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6322638, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=37.3096446700508, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=37.3096446700508, Blast_Score=221, Evalue=8e-58,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42903; Mature: 42903

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQDAAAHAPEPQAPEPWAEPVATALLVLADGTILEGFGIGQTGVADGEVCFNTAMTGYQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
EILTDPSYAGQIVTFTFPHIGNVGTNDEDLESLEAAPASGVRGTVVASAVTNPSNWRSSS
HHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
HLDGWLRARGIVGITGVDTRALTARIRDQGMPNAVIANDPEGKFDREALKARAAALAPME
CCCHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
GLDLVPPVTSRASSEWSETVWALGNGYGKRAPGEGLKVVAIDYGVKRNILRLLAQAGCDV
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCE
TVVPATTTADEILALKPDGVFLSNGPGDPAATGAYAVPVIRKLLDEKVPTFGICLGHQLM
EEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHH
GLALGGRTVKMGQGHHGANHPVKDKTTGKVEIVSMNHGFAVDPGSLPETAVETHVSLFDG
HHEECCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHEEEEC
SNCGLTLTDRPAFSVQHHPEASPGPRDSHYLFERFVTLMRSGAPETAKGA
CCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLQDAAAHAPEPQAPEPWAEPVATALLVLADGTILEGFGIGQTGVADGEVCFNTAMTGYQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
EILTDPSYAGQIVTFTFPHIGNVGTNDEDLESLEAAPASGVRGTVVASAVTNPSNWRSSS
HHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCC
HLDGWLRARGIVGITGVDTRALTARIRDQGMPNAVIANDPEGKFDREALKARAAALAPME
CCCHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCC
GLDLVPPVTSRASSEWSETVWALGNGYGKRAPGEGLKVVAIDYGVKRNILRLLAQAGCDV
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCE
TVVPATTTADEILALKPDGVFLSNGPGDPAATGAYAVPVIRKLLDEKVPTFGICLGHQLM
EEEECCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHH
GLALGGRTVKMGQGHHGANHPVKDKTTGKVEIVSMNHGFAVDPGSLPETAVETHVSLFDG
HHEECCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCHHHHHHHHEEEEC
SNCGLTLTDRPAFSVQHHPEASPGPRDSHYLFERFVTLMRSGAPETAKGA
CCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]