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The map label for this gene is greA [H]

Identifier: 170747000

GI number: 170747000

Start: 608989

End: 609528

Strand: Reverse

Name: greA [H]

Synonym: Mrad2831_0566

Alternate gene names: 170747000

Gene position: 609528-608989 (Counterclockwise)

Preceding gene: 170747001

Following gene: 170746999

Centisome position: 10.03

GC content: 68.15

Gene sequence:

>540_bases
ATGGCCCGGGGAGAGTGCTTCACTCTCGGCCGGGCCTCTTCATTGTGGCGCGAGACGATGGCGATAGACAAGGTTCCGAT
CACCGTGCGCGGGCTGGCAGCCCTCGAAGAGGAGCTGAAGCACCGCCAGCAGGTGGAGCGCCAGCGCATCATCCAGGCGA
TCGCGGAAGCCCGCGCGCTCGGCGACCTGTCGGAGAACGCCGAGTACCACGCCGCCAAGGAGGCGCAGTCCCACAACGAG
GGCCGCGTCCTCGAGCTCGAGAGCATGATCGCGCGGGCCGAAGTGATCGACGTCACGAAGCTCTCGGGCGACAGGATCAA
GTTCGGCGCCACCGTGAAGCTCGTCGACGAGGACACGGAGGAGGAGAAGACCTACCAGATCGTGGGCGAGCCGGAGGCCG
ACGTGCGCTCGGGCCGCGTCTCGATCACCTCGCCGATCGCCCGCGCGCTGATCGGCAAAGGCGTCGGCGACACGGTCGAG
GTCACGACCCCCGGTGGCGGCAAGTCCTACGAGGTCGTCGCCGTCCAGTACGGCGGCTGA

Upstream 100 bases:

>100_bases
GGCCGAGGGGATCAAGGCCTATCTAGAAGGCGATCTCAAGGTCCGCGCGTTGCAGGAATACTTCGCGGCCTGAACCTCAG
GGTTCCGCTGACTTCATTTC

Downstream 100 bases:

>100_bases
GCCGATGCGCCCGCGTTCCTGGGGCCGGCTCGCCGGCCTGATCCTCGGTGCCGTCGCGGGATCCGCGGCGGCGAGCGACT
TCCCGCCGACTCCGGTCTTC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MARGECFTLGRASSLWRETMAIDKVPITVRGLAALEEELKHRQQVERQRIIQAIAEARALGDLSENAEYHAAKEAQSHNE
GRVLELESMIARAEVIDVTKLSGDRIKFGATVKLVDEDTEEEKTYQIVGEPEADVRSGRVSITSPIARALIGKGVGDTVE
VTTPGGGKSYEVVAVQYGG

Sequences:

>Translated_179_residues
MARGECFTLGRASSLWRETMAIDKVPITVRGLAALEEELKHRQQVERQRIIQAIAEARALGDLSENAEYHAAKEAQSHNE
GRVLELESMIARAEVIDVTKLSGDRIKFGATVKLVDEDTEEEKTYQIVGEPEADVRSGRVSITSPIARALIGKGVGDTVE
VTTPGGGKSYEVVAVQYGG
>Mature_178_residues
ARGECFTLGRASSLWRETMAIDKVPITVRGLAALEEELKHRQQVERQRIIQAIAEARALGDLSENAEYHAAKEAQSHNEG
RVLELESMIARAEVIDVTKLSGDRIKFGATVKLVDEDTEEEKTYQIVGEPEADVRSGRVSITSPIARALIGKGVGDTVEV
TTPGGGKSYEVVAVQYGG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=51.2987012987013, Blast_Score=156, Evalue=9e-40,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=34.1935483870968, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19450; Mature: 19319

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGECFTLGRASSLWRETMAIDKVPITVRGLAALEEELKHRQQVERQRIIQAIAEARAL
CCCCCEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLSENAEYHAAKEAQSHNEGRVLELESMIARAEVIDVTKLSGDRIKFGATVKLVDEDTE
CCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHEEEECCCCEEEECCEEEEECCCCC
EEKTYQIVGEPEADVRSGRVSITSPIARALIGKGVGDTVEVTTPGGGKSYEVVAVQYGG
CCCEEEEECCCCCCHHCCEEEEHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECC
>Mature Secondary Structure 
ARGECFTLGRASSLWRETMAIDKVPITVRGLAALEEELKHRQQVERQRIIQAIAEARAL
CCCCEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDLSENAEYHAAKEAQSHNEGRVLELESMIARAEVIDVTKLSGDRIKFGATVKLVDEDTE
CCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHEEEECCCCEEEECCEEEEECCCCC
EEKTYQIVGEPEADVRSGRVSITSPIARALIGKGVGDTVEVTTPGGGKSYEVVAVQYGG
CCCEEEEECCCCCCHHCCEEEEHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA