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The map label for this gene is groL [H]

Identifier: 170746968

GI number: 170746968

Start: 571771

End: 573411

Strand: Reverse

Name: groL [H]

Synonym: Mrad2831_0534

Alternate gene names: 170746968

Gene position: 573411-571771 (Counterclockwise)

Preceding gene: 170746969

Following gene: 170746960

Centisome position: 9.43

GC content: 66.97

Gene sequence:

>1641_bases
ATGGCAGCGAAAGACGTTCGTTTCTCCGCCGACGCCCGCGATAAGATGCTGCGCGGCGTCGACATCCTCGCGGATGCGGT
GAAGGTGACGCTGGGCCCGAAGGGCCGCAACGTCGTGATCGAGAAGAGCTTCGGCGCCCCGCGCATCACCAAGGACGGTG
TGACGGTCGCCAAGGAGATCGAGCTCGCCGACAAGTTCGAGAACATGGGCGCCCAGATGGTGCGCGAAGTGGCCTCGAAG
ACCAACGACATCGCGGGTGACGGCACCACCACCGCGACCGTGCTGGCCCAGGCCATCGTCCGCGAGGGCGCCAAGTACGT
CGCCGCCGGCATCAACCCGATGGACCTGAAGCGCGGCATCGACCTCGCCACCCAGGCCGCCGTCAAGGACATCATCGCGC
GCGCCAAGAAGGTCTCGACCTCCGACGAGGTCGCCCAGGTCGGCACGATCTCCGCCAACGGCGACAAGGAGATCGGCGAG
ATGATCGCCCACGCCATGCAGAAGGTGGGCAACGAGGGCGTGATCACGGTCGAGGAGGCCAAGACGGCCGAGACCGAGCT
CGACGTCGTCGAGGGCATGCAGTTCGACCGCGGCTACCTCTCCCCGTACTTCATCACGAACGCGGAGAAGATGGTCGCCG
AGCTCGAGGACCCCTACATCCTCATCCACGAGAAGAAGCTCTCTTCGCTCCAGGCCATGCTGCCGGTGCTCGAGGCCGTT
GTTCAGACCGGCAAGCCGCTCCTGATCATCGCCGAGGACATCGAGGGCGAGGCGCTCGCCACGCTCGTCGTCAACAAGCT
CCGCGGCGGCCTCAAGGTCGCGGCCGTCAAGGCGCCGGGCTTCGGTGATCGCCGCAAGGCGATGCTCGAGGACATCGCGA
TCCTGACCAAGGGCCAGATGATCGCCGAGGATCTCGGCATCAAGCTCGAGAACGTGACGCTCCCCATGCTCGGCCGCGCC
AAGCGCGTGCGTATCGAGAAGGAGAACACCACGATCATCGACGGCGTCGGCGAGAAGTCCGACATCGAGGGCCGCATCTC
GCAGATCAAGGCGCAGATCGAGGAGACCACCTCGGACTACGACCGTGAGAAGCTGCAGGAGCGTCTGGCCAAGCTCGCGG
GCGGCGTCGCGGTGATCCGCGTCGGCGGCGCGACCGAGGTCGAGGTGAAGGAGAAGAAGGACCGCGTCGACGACGCCCTC
AACGCCACCCGCGCGGCGGTCGAGGAAGGCATCGTCCCCGGCGGCGGCACCGCGCTGCTGCGCGCCAAGAAGGCGGTCGC
CGAGCTGAAGTCCGACATCCCGGACGTCCAGGCCGGCATCAAGATCGTCCTCAAGGCTCTCGAGGCCCCGCTCCGCCAGA
TCGCCCAGAACGCGGGCGTGGAAGGCTCGATCGTGGTCGGCAAGATCACCGACAACACCTCCTCGGAGACCTTCGGCTTC
AACGCCCAGACCGAAGAGTACGTCGACATGATCCAGGCCGGCATCGTCGACCCGGCCAAGGTGGTGCGCACCGCTCTGCA
GGACGCGGCTTCGGTCGCCGGCCTGCTGGTCACCACCGAGGCGATGGTCGCCGACGCGCCGAAGAAGGACAGCCCCGCTC
CGGCCATGCCGGGCGGCGGCATGGGCGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
CCGACATCATGGGCGTCGTCGCCCTCTGATCGGATCACCCGTCATCGCCCTTTGAGGCCGTCCCGGCCTTGGGGTTCAAC
CAGTAGCTAAGAGGCACACA

Downstream 100 bases:

>100_bases
GTCCGCGCCACCGCGCATGACGGGGGAAAGGGGTCGCCGTGAGGCGGCCCCTTTTCTTTTGATGGCGGCACGCTCCAGCG
GCGCGCGAGGCGCAGTCCGG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MAAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELADKFENMGAQMVREVASK
TNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGIDLATQAAVKDIIARAKKVSTSDEVAQVGTISANGDKEIGE
MIAHAMQKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFITNAEKMVAELEDPYILIHEKKLSSLQAMLPVLEAV
VQTGKPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQMIAEDLGIKLENVTLPMLGRA
KRVRIEKENTTIIDGVGEKSDIEGRISQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVPGGGTALLRAKKAVAELKSDIPDVQAGIKIVLKALEAPLRQIAQNAGVEGSIVVGKITDNTSSETFGF
NAQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSPAPAMPGGGMGGMDF

Sequences:

>Translated_546_residues
MAAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELADKFENMGAQMVREVASK
TNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGIDLATQAAVKDIIARAKKVSTSDEVAQVGTISANGDKEIGE
MIAHAMQKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFITNAEKMVAELEDPYILIHEKKLSSLQAMLPVLEAV
VQTGKPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQMIAEDLGIKLENVTLPMLGRA
KRVRIEKENTTIIDGVGEKSDIEGRISQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVPGGGTALLRAKKAVAELKSDIPDVQAGIKIVLKALEAPLRQIAQNAGVEGSIVVGKITDNTSSETFGF
NAQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSPAPAMPGGGMGGMDF
>Mature_545_residues
AAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEIELADKFENMGAQMVREVASKT
NDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGIDLATQAAVKDIIARAKKVSTSDEVAQVGTISANGDKEIGEM
IAHAMQKVGNEGVITVEEAKTAETELDVVEGMQFDRGYLSPYFITNAEKMVAELEDPYILIHEKKLSSLQAMLPVLEAVV
QTGKPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQMIAEDLGIKLENVTLPMLGRAK
RVRIEKENTTIIDGVGEKSDIEGRISQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALN
ATRAAVEEGIVPGGGTALLRAKKAVAELKSDIPDVQAGIKIVLKALEAPLRQIAQNAGVEGSIVVGKITDNTSSETFGFN
AQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSPAPAMPGGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=529, Percent_Identity=54.820415879017, Blast_Score=564, Evalue=1e-161,
Organism=Homo sapiens, GI31542947, Length=529, Percent_Identity=54.820415879017, Blast_Score=564, Evalue=1e-161,
Organism=Homo sapiens, GI5453607, Length=546, Percent_Identity=23.992673992674, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI261399877, Length=518, Percent_Identity=23.5521235521236, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI261399875, Length=479, Percent_Identity=23.7995824634656, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=67.7966101694915, Blast_Score=699, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=53.3962264150943, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=54.2452830188679, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17564182, Length=548, Percent_Identity=21.3503649635036, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=56.2737642585551, Blast_Score=585, Evalue=1e-168,
Organism=Saccharomyces cerevisiae, GI6319518, Length=469, Percent_Identity=21.3219616204691, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24641193, Length=529, Percent_Identity=55.9546313799622, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24641191, Length=529, Percent_Identity=55.9546313799622, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI45550936, Length=527, Percent_Identity=53.3206831119545, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45550132, Length=527, Percent_Identity=53.3206831119545, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI45550935, Length=527, Percent_Identity=53.3206831119545, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI17864606, Length=547, Percent_Identity=46.9835466179159, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24584129, Length=538, Percent_Identity=37.546468401487, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI19921262, Length=538, Percent_Identity=37.546468401487, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57847; Mature: 57716

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ELADKFENMGAQMVREVASKTNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHCC
DLATQAAVKDIIARAKKVSTSDEVAQVGTISANGDKEIGEMIAHAMQKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELDVVEGMQFDRGYLSPYFITNAEKMVAELEDPYILIHEKKLSSLQAMLPVLEAV
CCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHH
VQTGKPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQM
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHH
IAEDLGIKLENVTLPMLGRAKRVRIEKENTTIIDGVGEKSDIEGRISQIKAQIEETTSDY
HHHHHCCEEECEEECCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVPGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAKKAVAELKSDIPDVQAGIKIVLKALEAPLRQIAQNAGVEGSIVVGKITDNTSSETFGF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
NAQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSPAPAMPGGG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MGGMDF
CCCCCC
>Mature Secondary Structure 
AAKDVRFSADARDKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVTVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCCHHHHHH
ELADKFENMGAQMVREVASKTNDIAGDGTTTATVLAQAIVREGAKYVAAGINPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHCC
DLATQAAVKDIIARAKKVSTSDEVAQVGTISANGDKEIGEMIAHAMQKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELDVVEGMQFDRGYLSPYFITNAEKMVAELEDPYILIHEKKLSSLQAMLPVLEAV
CCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHH
VQTGKPLLIIAEDIEGEALATLVVNKLRGGLKVAAVKAPGFGDRRKAMLEDIAILTKGQM
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHH
IAEDLGIKLENVTLPMLGRAKRVRIEKENTTIIDGVGEKSDIEGRISQIKAQIEETTSDY
HHHHHCCEEECEEECCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVPGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAKKAVAELKSDIPDVQAGIKIVLKALEAPLRQIAQNAGVEGSIVVGKITDNTSSETFGF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
NAQTEEYVDMIQAGIVDPAKVVRTALQDAASVAGLLVTTEAMVADAPKKDSPAPAMPGGG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MGGMDF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA