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The map label for this gene is artJ [H]

Identifier: 170746806

GI number: 170746806

Start: 394626

End: 395456

Strand: Reverse

Name: artJ [H]

Synonym: Mrad2831_0360

Alternate gene names: 170746806

Gene position: 395456-394626 (Counterclockwise)

Preceding gene: 170746808

Following gene: 170746805

Centisome position: 6.51

GC content: 66.31

Gene sequence:

>831_bases
ATGAAAGCGGGCGCCGGCTTCCGCCGCGTGACCCGGGCAATGCACGGGAGCGGCACCATGAGAAGACGGTTGGCGAAGCT
GTGTCTGTCGTCGGCGCTGCTCCTCGGCATCCTCGGGGCCGCCGTCGGCGCGCAGCCCCTGAAGGTGGGTTCGACGCCCA
CGGGTCTGCCCTTCACCTTCCTCGACACCAAGACGAACACCATTCAGGGCGTGATGGTCGACCTCGTCAACGCGGTCAGC
AAGGAAGCCGGCTTCACGGTCGCGGTGGAGCCGCTGCAATTCTCCACGCTGATCCCGGCCCTCACTGCCTCGAAGATCGA
CATCATCGCGGCGGCGATGTTCATCACGCCGCAGCGCAAGGAGGTCGTGTCGTTCTCGGCGCCCGTCTACAGCTACGGCG
AGGGGCTGATCGTGCCGAAGACCGACACGAAGGATTACACGTCCTTCGCCGACCTGAAGGGTGAGACCGTCGGCGCCCAG
GTCGGCACCGCCTTCGTCGAGCCGCTGAAGAAGTCGGGGCTCTTCAACGAGGTGAAGATCTACGACGCGATCCCCGACAT
CATCCGCGACGTGAATTCCGGGCGGCTGAAGGCCGGCTTCGCCGACGCGCCGATCCTGGCCTACTACGTGAAGCAGGGGC
TGTTCCCCGGCGTCCGCCTGGTCGAGACCTTCAAGCCGACCGTGGTCGCCTCGGTGGGTCTCAGCGTCCGCAAGACCGAC
ACCGAACTCTTGGCCAAGATCGACGCGGCGCTCGCCAAGCTGAAAGCCGACGGGACGCTCCAGGCGATCCTCACCAAGTG
GGGTCTGCCGCCGTCGGCCGACCGGTCCTGA

Upstream 100 bases:

>100_bases
GGGATGCTCACGGTCACGCCAGCCTGCTTCTCCTAGGCTTCTTCATGCACGTGAAAGTCGCTTGCGTCGATACCGAAAAC
ATGCGGTATTCTTCACGAGA

Downstream 100 bases:

>100_bases
CCTTCATGCGCGAGTTCCTCGCCGACGCCCAGGACTACCTGCCGATCCTGCTGCAGGGCGTGCAGCTCACGATCCTGATC
ACGATCGCGTCCCTGGTGGT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKAGAGFRRVTRAMHGSGTMRRRLAKLCLSSALLLGILGAAVGAQPLKVGSTPTGLPFTFLDTKTNTIQGVMVDLVNAVS
KEAGFTVAVEPLQFSTLIPALTASKIDIIAAAMFITPQRKEVVSFSAPVYSYGEGLIVPKTDTKDYTSFADLKGETVGAQ
VGTAFVEPLKKSGLFNEVKIYDAIPDIIRDVNSGRLKAGFADAPILAYYVKQGLFPGVRLVETFKPTVVASVGLSVRKTD
TELLAKIDAALAKLKADGTLQAILTKWGLPPSADRS

Sequences:

>Translated_276_residues
MKAGAGFRRVTRAMHGSGTMRRRLAKLCLSSALLLGILGAAVGAQPLKVGSTPTGLPFTFLDTKTNTIQGVMVDLVNAVS
KEAGFTVAVEPLQFSTLIPALTASKIDIIAAAMFITPQRKEVVSFSAPVYSYGEGLIVPKTDTKDYTSFADLKGETVGAQ
VGTAFVEPLKKSGLFNEVKIYDAIPDIIRDVNSGRLKAGFADAPILAYYVKQGLFPGVRLVETFKPTVVASVGLSVRKTD
TELLAKIDAALAKLKADGTLQAILTKWGLPPSADRS
>Mature_276_residues
MKAGAGFRRVTRAMHGSGTMRRRLAKLCLSSALLLGILGAAVGAQPLKVGSTPTGLPFTFLDTKTNTIQGVMVDLVNAVS
KEAGFTVAVEPLQFSTLIPALTASKIDIIAAAMFITPQRKEVVSFSAPVYSYGEGLIVPKTDTKDYTSFADLKGETVGAQ
VGTAFVEPLKKSGLFNEVKIYDAIPDIIRDVNSGRLKAGFADAPILAYYVKQGLFPGVRLVETFKPTVVASVGLSVRKTD
TELLAKIDAALAKLKADGTLQAILTKWGLPPSADRS

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=32.3886639676113, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=31.4285714285714, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=27.1653543307087, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=27.5862068965517, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=27.0042194092827, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29233; Mature: 29233

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAGAGFRRVTRAMHGSGTMRRRLAKLCLSSALLLGILGAAVGAQPLKVGSTPTGLPFTF
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEE
LDTKTNTIQGVMVDLVNAVSKEAGFTVAVEPLQFSTLIPALTASKIDIIAAAMFITPQRK
ECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVVSFSAPVYSYGEGLIVPKTDTKDYTSFADLKGETVGAQVGTAFVEPLKKSGLFNEVKI
HHHEECCCHHHCCCCEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
YDAIPDIIRDVNSGRLKAGFADAPILAYYVKQGLFPGVRLVETFKPTVVASVGLSVRKTD
HHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCEEECHH
TELLAKIDAALAKLKADGTLQAILTKWGLPPSADRS
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAGAGFRRVTRAMHGSGTMRRRLAKLCLSSALLLGILGAAVGAQPLKVGSTPTGLPFTF
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEE
LDTKTNTIQGVMVDLVNAVSKEAGFTVAVEPLQFSTLIPALTASKIDIIAAAMFITPQRK
ECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVVSFSAPVYSYGEGLIVPKTDTKDYTSFADLKGETVGAQVGTAFVEPLKKSGLFNEVKI
HHHEECCCHHHCCCCEEEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
YDAIPDIIRDVNSGRLKAGFADAPILAYYVKQGLFPGVRLVETFKPTVVASVGLSVRKTD
HHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCEEECHH
TELLAKIDAALAKLKADGTLQAILTKWGLPPSADRS
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]