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The map label for this gene is braC [H]

Identifier: 170746746

GI number: 170746746

Start: 323940

End: 325151

Strand: Reverse

Name: braC [H]

Synonym: Mrad2831_0299

Alternate gene names: 170746746

Gene position: 325151-323940 (Counterclockwise)

Preceding gene: 170746747

Following gene: 170746745

Centisome position: 5.35

GC content: 64.44

Gene sequence:

>1212_bases
ATGGTCTTCGCAGGTCGCGCTGCAGCCGCGGTGCTGTTATTGACGATGGTGTCGGCCCCGGCGCGGGCCGCGGACCCGAT
CAAGATCGGGCTGAGCGGCCCCTTCACGGGTGGGTCGTCTTCGATGGGTGTCTCGATGCGGGACGGCGCCAAGCTCGCCG
CCGCCGAGATCAACAAGGCCGGCGGCGTGCTCGGCCGGCCGATCCAGCTCGTGGAGCGCGACGACGAGGCCAAGAACGAG
GTCGGTGCGCAGGTCGCTCAGGAGCTGATCAACAAGGAGAAGGTGGTCGCCACCGTCGGGTTCATCAACACCGGCGTCGC
GCTGGCCGCCCAGCGCTTCTACCAGGAAGCCGAGATTCCCGTCTTCAACAACGTCGCGACCGGGACGATCATCACCAACC
AGTTCAAGGAGCCGGATTACGACGCGAACTACGTCTTCCGGAATTCCGCCTACGACGTGCTCCAGGCCGGCCTGATGGTC
GACGAGGCGCTCGCCCAGGGCCACAAGAAGATCGCGATCCTCGCCGACTCGACCAATTACGGTCAGCTAGGCCGCGAGGA
CATCGAGAAGTACCTCGCGACCAAGAACGTGAAGCCCGTCGCGGTCGAGAAGTTCAACATCAAGGACGTCGACATGACGG
CCCAGCTGCTGAAGGCGCAGCAGGCCGGCGCCGAGGTGATCCTCGCCTACGGCATCGGGCCGGAGCTGGCCCAGATCGCC
AACGGCATGGCCAAGCTCGGCTGGAAGGTCCCGATGATCACCAGCTGGCCGTCCTCGATGCAGAGCTTCATCGACATCGC
CGGCACGAACGGCAACGGCGTGATCATGCCGCAGACCTTCATCGAGGATCAGACCCTGCCGAAGCGCAAGGCGTTCCTGG
AGAACTACTACAAGACCTACGGCGTGACGAAGATCCCGACACCCGTGGCCGGCGCCCAGGGTTACGACTCGATCTACCTG
CTGGCCGCGGCGATCAAGCAGGCCGGCGGCACCGACGGCGCGAAGATCCGCACGGCGCTGGAGAATTTGAACGAGAAGGT
CGAGGGCGTCGTCACTACCTACGACAAGCCCTTCACCGCCAAGGACCACAACGCGATCTCGCGCAACATGGTCGTGTTCG
GGAAGGTGCAGGACGGCAAGATCGTCTACGCGAAGGACGAGGACGCCAAGAACGCGGGCGTGGTCCGCGTCAAGGAGAAG
TCCTCGAACTGA

Upstream 100 bases:

>100_bases
AAAATGGTAGCTTGACGGCTCGACCTGAGTCGGGAGATCAGCCTTCCAGAGTGCCACGTCGAAGGGCACACCGCGCGTGC
GGAATCTTGGAGAGGAAACG

Downstream 100 bases:

>100_bases
GGCTCCGGTGAGGCCGCGCCTTCCCGGGCGCGGCCGCCGCTACGCAGCACGGTTGCGCGGCCCTCGCCGCGCGACCGGAC
GTCCCTCCGGTGACAGCGAT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MVFAGRAAAAVLLLTMVSAPARAADPIKIGLSGPFTGGSSSMGVSMRDGAKLAAAEINKAGGVLGRPIQLVERDDEAKNE
VGAQVAQELINKEKVVATVGFINTGVALAAQRFYQEAEIPVFNNVATGTIITNQFKEPDYDANYVFRNSAYDVLQAGLMV
DEALAQGHKKIAILADSTNYGQLGREDIEKYLATKNVKPVAVEKFNIKDVDMTAQLLKAQQAGAEVILAYGIGPELAQIA
NGMAKLGWKVPMITSWPSSMQSFIDIAGTNGNGVIMPQTFIEDQTLPKRKAFLENYYKTYGVTKIPTPVAGAQGYDSIYL
LAAAIKQAGGTDGAKIRTALENLNEKVEGVVTTYDKPFTAKDHNAISRNMVVFGKVQDGKIVYAKDEDAKNAGVVRVKEK
SSN

Sequences:

>Translated_403_residues
MVFAGRAAAAVLLLTMVSAPARAADPIKIGLSGPFTGGSSSMGVSMRDGAKLAAAEINKAGGVLGRPIQLVERDDEAKNE
VGAQVAQELINKEKVVATVGFINTGVALAAQRFYQEAEIPVFNNVATGTIITNQFKEPDYDANYVFRNSAYDVLQAGLMV
DEALAQGHKKIAILADSTNYGQLGREDIEKYLATKNVKPVAVEKFNIKDVDMTAQLLKAQQAGAEVILAYGIGPELAQIA
NGMAKLGWKVPMITSWPSSMQSFIDIAGTNGNGVIMPQTFIEDQTLPKRKAFLENYYKTYGVTKIPTPVAGAQGYDSIYL
LAAAIKQAGGTDGAKIRTALENLNEKVEGVVTTYDKPFTAKDHNAISRNMVVFGKVQDGKIVYAKDEDAKNAGVVRVKEK
SSN
>Mature_403_residues
MVFAGRAAAAVLLLTMVSAPARAADPIKIGLSGPFTGGSSSMGVSMRDGAKLAAAEINKAGGVLGRPIQLVERDDEAKNE
VGAQVAQELINKEKVVATVGFINTGVALAAQRFYQEAEIPVFNNVATGTIITNQFKEPDYDANYVFRNSAYDVLQAGLMV
DEALAQGHKKIAILADSTNYGQLGREDIEKYLATKNVKPVAVEKFNIKDVDMTAQLLKAQQAGAEVILAYGIGPELAQIA
NGMAKLGWKVPMITSWPSSMQSFIDIAGTNGNGVIMPQTFIEDQTLPKRKAFLENYYKTYGVTKIPTPVAGAQGYDSIYL
LAAAIKQAGGTDGAKIRTALENLNEKVEGVVTTYDKPFTAKDHNAISRNMVVFGKVQDGKIVYAKDEDAKNAGVVRVKEK
SSN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43088; Mature: 43088

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFAGRAAAAVLLLTMVSAPARAADPIKIGLSGPFTGGSSSMGVSMRDGAKLAAAEINKA
CEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHC
GGVLGRPIQLVERDDEAKNEVGAQVAQELINKEKVVATVGFINTGVALAAQRFYQEAEIP
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHEEEEECHHHHHHHHHHHHHHHHCCCC
VFNNVATGTIITNQFKEPDYDANYVFRNSAYDVLQAGLMVDEALAQGHKKIAILADSTNY
EECCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
GQLGREDIEKYLATKNVKPVAVEKFNIKDVDMTAQLLKAQQAGAEVILAYGIGPELAQIA
CCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
NGMAKLGWKVPMITSWPSSMQSFIDIAGTNGNGVIMPQTFIEDQTLPKRKAFLENYYKTY
HHHHHCCCCCCEEECCHHHHHHHHEEECCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHC
GVTKIPTPVAGAQGYDSIYLLAAAIKQAGGTDGAKIRTALENLNEKVEGVVTTYDKPFTA
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCC
KDHNAISRNMVVFGKVQDGKIVYAKDEDAKNAGVVRVKEKSSN
CCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MVFAGRAAAAVLLLTMVSAPARAADPIKIGLSGPFTGGSSSMGVSMRDGAKLAAAEINKA
CEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHC
GGVLGRPIQLVERDDEAKNEVGAQVAQELINKEKVVATVGFINTGVALAAQRFYQEAEIP
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCHHEEEEECHHHHHHHHHHHHHHHHCCCC
VFNNVATGTIITNQFKEPDYDANYVFRNSAYDVLQAGLMVDEALAQGHKKIAILADSTNY
EECCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
GQLGREDIEKYLATKNVKPVAVEKFNIKDVDMTAQLLKAQQAGAEVILAYGIGPELAQIA
CCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
NGMAKLGWKVPMITSWPSSMQSFIDIAGTNGNGVIMPQTFIEDQTLPKRKAFLENYYKTY
HHHHHCCCCCCEEECCHHHHHHHHEEECCCCCEEECCHHHCCCCCCCHHHHHHHHHHHHC
GVTKIPTPVAGAQGYDSIYLLAAAIKQAGGTDGAKIRTALENLNEKVEGVVTTYDKPFTA
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCC
KDHNAISRNMVVFGKVQDGKIVYAKDEDAKNAGVVRVKEKSSN
CCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]