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The map label for this gene is cfxB [H]

Identifier: 170746606

GI number: 170746606

Start: 155734

End: 156804

Strand: Reverse

Name: cfxB [H]

Synonym: Mrad2831_0156

Alternate gene names: 170746606

Gene position: 156804-155734 (Counterclockwise)

Preceding gene: 170746607

Following gene: 170746605

Centisome position: 2.58

GC content: 66.76

Gene sequence:

>1071_bases
ATGGCACGCATCACCCTCCGGCAGCTCCTCGATCACGCCGCTGAGTACGAGTACGGCGTGCCCGCCTTCAACCTGAACAA
CATGGAGCAGGGGCTCGCTATCATGGCGGCCGCCGACGCCACCGACTCACCGGTGATCCTCCAGGCGAGCCGCGGCGCCC
GGGCCTACGCCAACGACGTCGTGCTGGCCAAGCTGATCGACGGCCTCGTCGAGATCTACCCGCACATTCCGGTCTGCATG
CATCTCGACCACGGCAACAACGAAGCCACCTGCGCCACCGCCATCCAGTACGGCTTCACCTCCGTGATGATGGACGGCTC
GCTGAAGGCCGACGGCAAGACCCCGGCCGACTACACCTACAACGTCGAGATCACCCGCAACGTCACCAAGATGGCCCACT
GGGCCGGTGTCTCGGTCGAGGGCGAGCTGGGCGTGCTCGGCTCGTTGGAGAGCGGCCAGGGCGAGGCCGAGGACGGCCAC
GGCGCCGAGGGCACCCTCAGCCACGACCAGCTCCTGACCGACCCGGACGAAGCGGTGAAGTTCGTCGAGGCCACGAAGGT
GGACGCGCTGGCCGTCGCCATGGGCACGAGCCACGGCGCCTACAAGTTCACCCGCAAGCCCGACGGCGAGGTGCTGGCGA
TGAACGTGATCGAGGAGATCCACCGCCGCCTGCCGACGACCCACCTCGTCATGCACGGCTCGTCGTCGGTGCCGCAGGAC
CTGCAGGACATCATCAACCAGTACGGCGGCCAGATGAAGCCGACCTGGGGCGTCCCGGTCGAGGAGATCCAGCGCGGCAT
CAAGCACGGCGTGCGCAAGATCAACATCGACACCGACAATCGCATGGCGATGACCGGCCAGATCCGGAAGGTGCTCACCG
AGAACCCGTCCGAGTTCGACCCGCGCAAGTACCTCAAGCCCGCCATGGAGGCGATGACGAAGCTCTGCAAGCAGCGCTTC
GAGGAGTTCAACACCGCCGGCAAGGGCTCGAAGATCCGCCCGATCTCCGTCGCCGAGATGGCCAAGCGCTACGCCAGCGG
CGCCCTCGACCCGAAGATCGGGCCGCAGTAG

Upstream 100 bases:

>100_bases
AGGCACCCGGCAGAGCGACACGATACAGCGCAGCTGAGGGTTTCTTGATACCCCCGGCGATCCGGCGGACGACGAAACGG
GAAACGGGAAGACACGCACG

Downstream 100 bases:

>100_bases
GGCGCGGGTTGCCGGTTTCGATGAGCAATAGCGCCCGTCTCGCCCTGCTCGGACCGCACGGTCTCGAAGCGGAAGGCATC
GCCGCTTTCGTGACGGCCCT

Product: fructose-1,6-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; Fructose-bisphosphate aldolase II [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MARITLRQLLDHAAEYEYGVPAFNLNNMEQGLAIMAAADATDSPVILQASRGARAYANDVVLAKLIDGLVEIYPHIPVCM
HLDHGNNEATCATAIQYGFTSVMMDGSLKADGKTPADYTYNVEITRNVTKMAHWAGVSVEGELGVLGSLESGQGEAEDGH
GAEGTLSHDQLLTDPDEAVKFVEATKVDALAVAMGTSHGAYKFTRKPDGEVLAMNVIEEIHRRLPTTHLVMHGSSSVPQD
LQDIINQYGGQMKPTWGVPVEEIQRGIKHGVRKINIDTDNRMAMTGQIRKVLTENPSEFDPRKYLKPAMEAMTKLCKQRF
EEFNTAGKGSKIRPISVAEMAKRYASGALDPKIGPQ

Sequences:

>Translated_356_residues
MARITLRQLLDHAAEYEYGVPAFNLNNMEQGLAIMAAADATDSPVILQASRGARAYANDVVLAKLIDGLVEIYPHIPVCM
HLDHGNNEATCATAIQYGFTSVMMDGSLKADGKTPADYTYNVEITRNVTKMAHWAGVSVEGELGVLGSLESGQGEAEDGH
GAEGTLSHDQLLTDPDEAVKFVEATKVDALAVAMGTSHGAYKFTRKPDGEVLAMNVIEEIHRRLPTTHLVMHGSSSVPQD
LQDIINQYGGQMKPTWGVPVEEIQRGIKHGVRKINIDTDNRMAMTGQIRKVLTENPSEFDPRKYLKPAMEAMTKLCKQRF
EEFNTAGKGSKIRPISVAEMAKRYASGALDPKIGPQ
>Mature_355_residues
ARITLRQLLDHAAEYEYGVPAFNLNNMEQGLAIMAAADATDSPVILQASRGARAYANDVVLAKLIDGLVEIYPHIPVCMH
LDHGNNEATCATAIQYGFTSVMMDGSLKADGKTPADYTYNVEITRNVTKMAHWAGVSVEGELGVLGSLESGQGEAEDGHG
AEGTLSHDQLLTDPDEAVKFVEATKVDALAVAMGTSHGAYKFTRKPDGEVLAMNVIEEIHRRLPTTHLVMHGSSSVPQDL
QDIINQYGGQMKPTWGVPVEEIQRGIKHGVRKINIDTDNRMAMTGQIRKVLTENPSEFDPRKYLKPAMEAMTKLCKQRFE
EFNTAGKGSKIRPISVAEMAKRYASGALDPKIGPQ

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=333, Percent_Identity=32.4324324324324, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI87082041, Length=329, Percent_Identity=31.0030395136778, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788072, Length=276, Percent_Identity=28.6231884057971, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789293, Length=368, Percent_Identity=23.0978260869565, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322790, Length=374, Percent_Identity=25.9358288770053, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006412
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 38791; Mature: 38659

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00602 ALDOLASE_CLASS_II_1 ; PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITLRQLLDHAAEYEYGVPAFNLNNMEQGLAIMAAADATDSPVILQASRGARAYANDV
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEECCCCCCCEEEEECCCCCHHHHHH
VLAKLIDGLVEIYPHIPVCMHLDHGNNEATCATAIQYGFTSVMMDGSLKADGKTPADYTY
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEE
NVEITRNVTKMAHWAGVSVEGELGVLGSLESGQGEAEDGHGAEGTLSHDQLLTDPDEAVK
EEEEECCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FVEATKVDALAVAMGTSHGAYKFTRKPDGEVLAMNVIEEIHRRLPTTHLVMHGSSSVPQD
HHHHHHHHEEEEEECCCCCCEEECCCCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCCHH
LQDIINQYGGQMKPTWGVPVEEIQRGIKHGVRKINIDTDNRMAMTGQIRKVLTENPSEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHHCCCCCCC
PRKYLKPAMEAMTKLCKQRFEEFNTAGKGSKIRPISVAEMAKRYASGALDPKIGPQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ARITLRQLLDHAAEYEYGVPAFNLNNMEQGLAIMAAADATDSPVILQASRGARAYANDV
CCCHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEEEECCCCCCCEEEEECCCCCHHHHHH
VLAKLIDGLVEIYPHIPVCMHLDHGNNEATCATAIQYGFTSVMMDGSLKADGKTPADYTY
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEE
NVEITRNVTKMAHWAGVSVEGELGVLGSLESGQGEAEDGHGAEGTLSHDQLLTDPDEAVK
EEEEECCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FVEATKVDALAVAMGTSHGAYKFTRKPDGEVLAMNVIEEIHRRLPTTHLVMHGSSSVPQD
HHHHHHHHEEEEEECCCCCCEEECCCCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCCHH
LQDIINQYGGQMKPTWGVPVEEIQRGIKHGVRKINIDTDNRMAMTGQIRKVLTENPSEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHHCCCCCCC
PRKYLKPAMEAMTKLCKQRFEEFNTAGKGSKIRPISVAEMAKRYASGALDPKIGPQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939098 [H]