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The map label for this gene is pyk

Identifier: 170762456

GI number: 170762456

Start: 224097

End: 225521

Strand: Direct

Name: pyk

Synonym: UPA3_0193

Alternate gene names: 170762456

Gene position: 224097-225521 (Clockwise)

Preceding gene: 170762202

Following gene: 170761945

Centisome position: 29.81

GC content: 22.88

Gene sequence:

>1425_bases
ATGTTTTCAAAAACACGCATTATTGCTACAGTAGGTCCAGCAACAACAAAATTGTTTTCTAATTGGTTAGAATTTAACGA
TTTAAATAATATAAATGATGCTCAACAAGTATGCATAAACATTAAAGATTTAATTTTAAGTGGTGTTAATATTTTTCGTA
TGAATTTAAGTCATGGCGACCAAAAAATACACCTTTTTCGTACACAATTAATTAAAAAAATTGCGGATGAATTAAAGATC
AAAGTTGAAATATTATTTGATACAAAAGGACCAGAAATTCGTGTTTGTGAAATGAGTGATAATAATTTTATTATTAAAAA
TAGTGAAGTTATAATACATTGTAAAGAAAAAGTTTTAGGTAGTTTTAATGAGTTTAGTGTTACTGATGCTACTGGACAAT
ACAATATGATTAGTGATGTTAAAATTAATCACCGAATTTTAATCGACGATGGAAAATTAATTTTAATTGTTAAAAAAATT
GATTTTCTAAAAAACATCATTTATACAACCGCTAAAAATAGTTATTCATTAAAAACAAATAAACGCTTAAATTTACCAGA
TGCCAATTATAGTTTGCCGTTTTTGTCAAAGAAAGATATTGATGATATAAATTTAGCAGTTAAATTAAAAATTCCTTATT
TGGCTTTATCGTTTATTTCTAATATTAAACAAATTAATGAGGTTAAACAACTTTTAAAAAAGTCCTCATTTATACCAAAA
TTAATTGCAAAAATTGAAACTCAAGAAGCAATTGATAATCTAGAAGAAATTATTAAAAATACTGATGGTATCATGGTTGC
GCGTGGGGATTTAGGGCTAGAAGTACCTTTTTATAAAATTCCTATTTATCAAAATAAAATTGTTGAATTATGCCATAAAT
ACAATAAATATTGTATTATAGCGACACAAATGTTAGATTCATTAGAACGTAATATTATCCCCACACGAGCTGAAGTCACA
GATGTTTATTATGCCGTTAAACAAAAGGTTAACGCAACAATGTTATCAGGCGAAACGGCCGCAGGAATTGATCCAATTAA
TGCTGTACAAGTTATGAAATCTATTATTTTAGAGACAGAAAATAATAATATAGAAAACGAAATAACATTTAAAAAAAATG
AATTATTAATTCCTAAGGAAGTTAAATTAAAAATTTTATCATTTCATAAATTAAAAATAAATAAACATAAATTATTAGTT
GTTAATTTAATTAGTAAAAATTCTAAACATTTAGTTTATGATATATTAAAATATGATTTTAATTTTAATATTTTATTTGT
TATAGATAAAAATCAAGAACATGATTTATTATTAAAAAATGCTAATTTTATTATTAGTGAAAATAAAATGTTAAAACAAG
AAGAATTATTAAAATCATTTAATATTGATTTTAATAATCATGAATTTTTATTTATTTCAATTTAA

Upstream 100 bases:

>100_bases
ATCAAAATGATATAAAAAACACAAATAATATGTATGTAGTTATTAATAAGTTTGATAATAAGTTAGAAGTTATTAAATTC
AATAAATAGAGGGAAAATTA

Downstream 100 bases:

>100_bases
AATAAAAATAATTTTATTATTAATATCTTTAATATAGTATAATTATAAAATATTCTGTCTATTTAAGCACAAATGTGTAA
TAAATAGGCTTTTTTCTATT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGDQKIHLFRTQLIKKIADELKI
KVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLGSFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKI
DFLKNIIYTTAKNSYSLKTNKRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPK
LIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCIIATQMLDSLERNIIPTRAEVT
DVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETENNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLV
VNLISKNSKHLVYDILKYDFNFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI

Sequences:

>Translated_474_residues
MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGDQKIHLFRTQLIKKIADELKI
KVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLGSFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKI
DFLKNIIYTTAKNSYSLKTNKRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPK
LIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCIIATQMLDSLERNIIPTRAEVT
DVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETENNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLV
VNLISKNSKHLVYDILKYDFNFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI
>Mature_474_residues
MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGDQKIHLFRTQLIKKIADELKI
KVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLGSFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKI
DFLKNIIYTTAKNSYSLKTNKRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPK
LIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCIIATQMLDSLERNIIPTRAEVT
DVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETENNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLV
VNLISKNSKHLVYDILKYDFNFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=368, Percent_Identity=32.6086956521739, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI32967597, Length=368, Percent_Identity=32.6086956521739, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI33286422, Length=376, Percent_Identity=32.9787234042553, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI33286420, Length=376, Percent_Identity=32.9787234042553, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI33286418, Length=376, Percent_Identity=32.9787234042553, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128732, Length=319, Percent_Identity=30.4075235109718, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI310128730, Length=319, Percent_Identity=30.4075235109718, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI310128736, Length=267, Percent_Identity=28.0898876404494, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310128734, Length=267, Percent_Identity=28.0898876404494, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310128738, Length=228, Percent_Identity=29.3859649122807, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1787965, Length=360, Percent_Identity=33.6111111111111, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788160, Length=370, Percent_Identity=32.7027027027027, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17544584, Length=364, Percent_Identity=35.1648351648352, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI71984406, Length=370, Percent_Identity=33.5135135135135, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17506829, Length=370, Percent_Identity=33.5135135135135, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71984413, Length=370, Percent_Identity=33.7837837837838, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17506831, Length=370, Percent_Identity=33.5135135135135, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324923, Length=363, Percent_Identity=34.1597796143251, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6319279, Length=354, Percent_Identity=34.4632768361582, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24648964, Length=368, Percent_Identity=33.9673913043478, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI28571814, Length=368, Percent_Identity=33.9673913043478, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24648966, Length=325, Percent_Identity=34.1538461538462, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24581235, Length=330, Percent_Identity=29.3939393939394, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24646914, Length=184, Percent_Identity=36.9565217391304, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54554; Mature: 54554

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGD
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
QKIHLFRTQLIKKIADELKIKVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLG
CEEHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEEEEECCCEEEEECCCEEEEECHHHCC
SFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKIDFLKNIIYTTAKNSYSLKTN
CCCCEEEECCCCCCCEEECEEECCEEEEECCCEEEEEEHHHHHHHHHHHCCCCCEEEECC
KRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPK
CEECCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCII
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCEEEECCCHHHHHHHHHHCCCEEEE
ATQMLDSLERNIIPTRAEVTDVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHEECC
NNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLVVNLISKNSKHLVYDILKYDF
CCCCCCEEEEECCCEECCHHHEEEEEEEEEEEECCCEEEEEEEECCCCCCEEEEEEHCCC
NFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI
CEEEEEEEECCCCCCEEEECCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure
MFSKTRIIATVGPATTKLFSNWLEFNDLNNINDAQQVCINIKDLILSGVNIFRMNLSHGD
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
QKIHLFRTQLIKKIADELKIKVEILFDTKGPEIRVCEMSDNNFIIKNSEVIIHCKEKVLG
CEEHHHHHHHHHHHHHHCEEEEEEEEECCCCEEEEEEECCCEEEEECCCEEEEECHHHCC
SFNEFSVTDATGQYNMISDVKINHRILIDDGKLILIVKKIDFLKNIIYTTAKNSYSLKTN
CCCCEEEECCCCCCCEEECEEECCEEEEECCCEEEEEEHHHHHHHHHHHCCCCCEEEECC
KRLNLPDANYSLPFLSKKDIDDINLAVKLKIPYLALSFISNIKQINEVKQLLKKSSFIPK
CEECCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LIAKIETQEAIDNLEEIIKNTDGIMVARGDLGLEVPFYKIPIYQNKIVELCHKYNKYCII
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCEEEECCCHHHHHHHHHHCCCEEEE
ATQMLDSLERNIIPTRAEVTDVYYAVKQKVNATMLSGETAAGIDPINAVQVMKSIILETE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHEECC
NNNIENEITFKKNELLIPKEVKLKILSFHKLKINKHKLLVVNLISKNSKHLVYDILKYDF
CCCCCCEEEEECCCEECCHHHEEEEEEEEEEEECCCEEEEEEEECCCCCCEEEEEEHCCC
NFNILFVIDKNQEHDLLLKNANFIISENKMLKQEELLKSFNIDFNNHEFLFISI
CEEEEEEEECCCCCCEEEECCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]