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The map label for this gene is eno

Identifier: 170762237

GI number: 170762237

Start: 221829

End: 223151

Strand: Direct

Name: eno

Synonym: UPA3_0191

Alternate gene names: 170762237

Gene position: 221829-223151 (Clockwise)

Preceding gene: 170762021

Following gene: 170762202

Centisome position: 29.51

GC content: 28.72

Gene sequence:

>1323_bases
ATGAAAATAATTAATTTATTAGCTTATCAAATTCTTGATTCGCGCGGCCAACCAACAGTAGCTGTTAAATTATTTTTAGA
GAACGATCAAAGTGTAATTGCAATGGTTCCATCAGGCGCTTCCACTGGAGCAAAGGAAGCATTAGAATTAAGAGATGGTG
ATGTAAATTATTTTTTTAATAAGTCTGTTAAATTAGCAATTCAAAATATTAATAATATTATTCGCCCTCATCTAATTAAT
AAAAATGTTTTAAATTTTTTTGAATTAGATAATTTATTAATTAATCTAGATGGTACTGAAAATAAAAGTAAATTAGGTGC
TAATGCTTTATTAGGTGTTTCTATAGCGATTGTTAAAGCGGGAGCAATAGCCGCTTCTAAACCTTTATATCAATATATTA
AAGAAGACTTAATGCATAATTATGATGTAAACTATTATGCTCCAATTCCGTTAATGAATTTTATTAATGGTGGGGCTCAC
GCAGATAATGATTTAGATATTCAAGAATTTATGATTGTTCCATTAAATGCAATTTCATTTTCTCAAGCGATTCAAATTGG
ATCAGAAATTTTTCATCAATTAGATAAACTTTTAAAAAGCAATCATTTAAGTACAACAAAGGGTGATGAAGGCGGTTTTG
CTCCAATGTTAAAAAATAATTATGTGACTTTAGAACTATTAGTTCATGCTATTAAAAAAGCACATTACCTTCCTTCAAAA
ACACAAGGAGTTTGTCTTGCCTTAGATGTCGCAGCTTCTGAATTGTATGAAAACGGAAAATATTTTTTTAAAAAGTCTTC
ATCTCATAACATTACTCTTGAACAAACATCATTCAGTAGTGATGAATGAATAAAGTATTGAAGTAAATTAGTATCTATGT
TTCCTATCATTTCGATCGAGGATTGTTTTGAGGAAAACGATTGAAATAGTTTTGCGTTATTTTTAAAAAATAACCCACAC
ATTCAAGTGGTTGGTGATGATTTATATTGCACAAACTTAAAGTATCTACAAAAAGGAATTGATTTTAAAGCAACAAATGC
TATTTTAATTAAACCTAATCAAATTGGTACAATTAGTGAAACGTTAGATGTTATTAAATTTGCACAAAAAAACAATATTA
ATACTATCATTTCGCATCGCTCTGGCGAAACTGAAGACACTTTTATTGCAGATTTAGCGATTGGTGTAGGTGCTGGGCAA
ATTAAAACAGGTTCATTATCACGTTCTGAACGGATAGCGAAGTATAATCGTATTTTAGAAATCGAACAAGAATTAAAGGA
TAAATTAATTTATGAACCAAGTAAATTTTTTAAATTTAGATAA

Upstream 100 bases:

>100_bases
AATATTGAAGACACTTGTTTAATTATTTGTCTTAATATTTTTTTAATTTTTAACATAAAATTAATGTATAAGTCTTTTAA
AAAAGAGGAATTAACAACAT

Downstream 100 bases:

>100_bases
AAATATTTTAATTATTACTTCAGGTGGTGATGCACCGGGTATGAATGCTTCATTAATTTCATTAATCCATAGATTAATGA
ATAATAATTTTAATGTTTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLIN
KNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAH
ADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSK
TQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDEWIKYWSKLVSMFPIISIEDCFEENDWNSFALFLKNNPH
IQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQ
IKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR

Sequences:

>Translated_440_residues
MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLIN
KNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAH
ADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSK
TQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDE*IKY*SKLVSMFPIISIEDCFEEND*NSFALFLKNNPH
IQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQ
IKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR
>Mature_440_residues
MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFNKSVKLAIQNINNIIRPHLIN
KNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKAGAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAH
ADNDLDIQEFMIVPLNAISFSQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSK
TQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDE*IKY*SKLVSMFPIISIEDCFEEND*NSFALFLKNNPH
IQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLDVIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQ
IKTGSLSRSERIAKYNRILEIEQELKDKLIYEPSKFFKFR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=47.1395881006865, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=47.1395881006865, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI5803011, Length=441, Percent_Identity=46.7120181405896, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI4503571, Length=439, Percent_Identity=46.4692482915718, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI301897479, Length=435, Percent_Identity=42.7586206896552, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI169201331, Length=348, Percent_Identity=25.2873563218391, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI169201757, Length=348, Percent_Identity=25.2873563218391, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI239744207, Length=348, Percent_Identity=25.2873563218391, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1789141, Length=438, Percent_Identity=47.4885844748858, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71995829, Length=441, Percent_Identity=46.031746031746, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17536383, Length=441, Percent_Identity=46.031746031746, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=42.1319796954315, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323985, Length=442, Percent_Identity=43.2126696832579, Blast_Score=332, Evalue=8e-92,
Organism=Saccharomyces cerevisiae, GI6324974, Length=442, Percent_Identity=43.2126696832579, Blast_Score=332, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6324969, Length=442, Percent_Identity=43.2126696832579, Blast_Score=332, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=42.6303854875283, Blast_Score=326, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=43.0839002267574, Blast_Score=309, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24580918, Length=443, Percent_Identity=45.5981941309255, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24580916, Length=443, Percent_Identity=45.5981941309255, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24580920, Length=443, Percent_Identity=45.5981941309255, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI24580914, Length=443, Percent_Identity=45.5981941309255, Blast_Score=341, Evalue=6e-94,
Organism=Drosophila melanogaster, GI281360527, Length=443, Percent_Identity=45.5981941309255, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI17137654, Length=443, Percent_Identity=45.5981941309255, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 48688; Mature: 48688

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFN
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEC
KSVKLAIQNINNIIRPHLINKNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKA
CCCEEEHHHHHHHHCCCCCCCHHHHHEEECCEEEEECCCCCCHHCCCCHHHHHEEEEEEC
GAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAHADNDLDIQEFMIVPLNAISF
CCHHCCHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCCCCCCHHHEEEEECCHHHH
SQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCC
TQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDEIKYSKLVSMFPIISIEDC
CCCEEEEEEHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEHHH
FEENDNSFALFLKNNPHIQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLD
HCCCCCEEEEEEECCCEEEEEECCEEECCHHHHHHCCCEEECCEEEECCCCCCCHHHHHH
VIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQIKTGSLSRSERIAKYNRILEIEQ
HHHHHHCCCCEEEEECCCCCCCCCEEEEHHEECCCCCEECCCCCHHHHHHHHHHHHHHHH
ELKDKLIYEPSKFFKFR
HHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEALELRDGDVNYFFN
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEEEC
KSVKLAIQNINNIIRPHLINKNVLNFFELDNLLINLDGTENKSKLGANALLGVSIAIVKA
CCCEEEHHHHHHHHCCCCCCCHHHHHEEECCEEEEECCCCCCHHCCCCHHHHHEEEEEEC
GAIAASKPLYQYIKEDLMHNYDVNYYAPIPLMNFINGGAHADNDLDIQEFMIVPLNAISF
CCHHCCHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCCCCCCCCCHHHEEEEECCHHHH
SQAIQIGSEIFHQLDKLLKSNHLSTTKGDEGGFAPMLKNNYVTLELLVHAIKKAHYLPSK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCC
TQGVCLALDVAASELYENGKYFFKKSSSHNITLEQTSFSSDEIKYSKLVSMFPIISIEDC
CCCEEEEEEHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEHHH
FEENDNSFALFLKNNPHIQVVGDDLYCTNLKYLQKGIDFKATNAILIKPNQIGTISETLD
HCCCCCEEEEEEECCCEEEEEECCEEECCHHHHHHCCCEEECCEEEECCCCCCCHHHHHH
VIKFAQKNNINTIISHRSGETEDTFIADLAIGVGAGQIKTGSLSRSERIAKYNRILEIEQ
HHHHHHCCCCEEEEECCCCCCCCCEEEEHHEECCCCCEECCCCCHHHHHHHHHHHHHHHH
ELKDKLIYEPSKFFKFR
HHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA