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The map label for this gene is hsdM

Identifier: 170762197

GI number: 170762197

Start: 124347

End: 125393

Strand: Direct

Name: hsdM

Synonym: UPA3_0101

Alternate gene names: 170762197

Gene position: 124347-125393 (Clockwise)

Preceding gene: 170762443

Following gene: 170762424

Centisome position: 16.54

GC content: 26.84

Gene sequence:

>1047_bases
GTGTTCGGTGATGCTTATGAATACTTAATGAGTATGTATGCTGCAAATGCAGGTAAATCAGGGGGAGAATTTTTCACCCC
TCAAGAAGTTAGTGAACTATTAGTAGAATTAACATTAATTGATTTTAATAATGAAAATAAAGATGTTAGAAGAAAAATTG
GAAAAGTTTATGATCCATGTTGTGGTTCTGGATCATTACTTTTAAAGTATGCAAAATTAAATGAAGGAGTTAAATTTTAT
GGTCAAGAAATTAATCTTACTACGTATAATTTAGCAAGAATTAATATGTTTTTGCACAATATAGGTTATGATAAATTTGA
TATCAAATTAGGTGATACATTACTAGATCCTAAACATAATGATGATAAACCTTTTGATGCAATTGTTTCAAATCCTCCAT
ATTCAACAAAATGGGAGGGAAAATCAAATCCTTTATTAGCTAACGATGAGCGATTTCATGTTACTCAATTAGCACCTAAA
GGAAAAGCTGATTTTGCTTTTGTATTGCATATACTTCATAATTTATCTTCTAGTGGAACAGCAGCTATAGTTATGTTTCC
AGGAACTTTATATCGAGATCATGCAGAACAAGATATTAGAAAATATTTAGTTGATAATGTTAATGTTGTAGATAGTGTGA
TTCAATTACCAGATAATCTGTTTTTTGGAACAAGTATTTCAACATGTATTATAGTTTTAAGAAAAAACAAAAACAACAAT
GATAATGCTAATGGTATCTTGTTTGTTGATGCATCAAAAGAATTTGTTAAATCTGGTATCAAAAACAAATTAACCAATGC
TAATATTAAAAAAATAGTTGATACGATTCGTTTCAAAAAAGAAGTAACTTATTTTTCAAAACTTGTATCACGAGAAGAAG
TTAAAAATAAAAACTATAATTTATCAGTTAACACATATGTGGAAAAAGAAGACACGTCAGAAAAAATTGATATTAAATTA
TTAAATATGCAAATTAAAGAAATTGTGGCTAAAATCGAAAAGTTAAGAAAAGAAATTGATGAAATTGTTTTAGAATTAGA
AGAATAA

Upstream 100 bases:

>100_bases
GTTTTTTAGTAATTTTTCTCAAAATAAAGCTCAAATATTTTGTGTTTAAATTAGAATTAATGAACTTGATCTAGGTAATT
ATCAAGATAATACAATCGAT

Downstream 100 bases:

>100_bases
ATTATGAATAAAATTGAAAAATTAATTAATGAGTTCTGCCCTAATGGAGTGGAGTTTAAAAAATTAAAAAATATAATTAC
AGTGGCTCCTAAATCACCTT

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPCCGSGSLLLKYAKLNEGVKFY
GQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHNDDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPK
GKADFAFVLHILHNLSSSGTAAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNN
DNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYNLSVNTYVEKEDTSEKIDIKL
LNMQIKEIVAKIEKLRKEIDEIVLELEE

Sequences:

>Translated_348_residues
MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPCCGSGSLLLKYAKLNEGVKFY
GQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHNDDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPK
GKADFAFVLHILHNLSSSGTAAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNN
DNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYNLSVNTYVEKEDTSEKIDIKL
LNMQIKEIVAKIEKLRKEIDEIVLELEE
>Mature_348_residues
MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPCCGSGSLLLKYAKLNEGVKFY
GQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHNDDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPK
GKADFAFVLHILHNLSSSGTAAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNN
DNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYNLSVNTYVEKEDTSEKIDIKL
LNMQIKEIVAKIEKLRKEIDEIVLELEE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=264, Percent_Identity=24.6212121212121, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 39548; Mature: 39548

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPC
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCC
CGSGSLLLKYAKLNEGVKFYGQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHN
CCCCHHEEEEHHHCCCCEEECCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCEECCCCCC
DDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPKGKADFAFVLHILHNLSSSGT
CCCCHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
AAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNN
EEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHEEEEEECCCCCC
DNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYN
CCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LSVNTYVEKEDTSEKIDIKLLNMQIKEIVAKIEKLRKEIDEIVLELEE
EEEEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFGDAYEYLMSMYAANAGKSGGEFFTPQEVSELLVELTLIDFNNENKDVRRKIGKVYDPC
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCC
CGSGSLLLKYAKLNEGVKFYGQEINLTTYNLARINMFLHNIGYDKFDIKLGDTLLDPKHN
CCCCHHEEEEHHHCCCCEEECCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCEECCCCCC
DDKPFDAIVSNPPYSTKWEGKSNPLLANDERFHVTQLAPKGKADFAFVLHILHNLSSSGT
CCCCHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
AAIVMFPGTLYRDHAEQDIRKYLVDNVNVVDSVIQLPDNLFFGTSISTCIIVLRKNKNNN
EEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHEEEEEECCCCCC
DNANGILFVDASKEFVKSGIKNKLTNANIKKIVDTIRFKKEVTYFSKLVSREEVKNKNYN
CCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LSVNTYVEKEDTSEKIDIKLLNMQIKEIVAKIEKLRKEIDEIVLELEE
EEEEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]