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The map label for this gene is rlmN [H]

Identifier: 170761929

GI number: 170761929

Start: 256711

End: 257721

Strand: Direct

Name: rlmN [H]

Synonym: UPA3_0221

Alternate gene names: 170761929

Gene position: 256711-257721 (Clockwise)

Preceding gene: 170762310

Following gene: 170761851

Centisome position: 34.15

GC content: 19.19

Gene sequence:

>1011_bases
ATGAAATTAAACAATCATAATCTAAAAGAAATTTATTCATTATCATTAAATGAACTTAAAGAAGAATTATTTAAATTAGG
CTTAAAATCTTTTATTAGCAAACAAATTTATTCTTGGATTTATCAGAAACGAATTTTTAATTTTGATAAATTTTCTAATA
TTGCTAAAAATAATGTAATAATTCTAAAAGAAAATTTTAGTAATAATCTATTAAAAATTAACAGTTATCAATCAAATTCT
GATGGTTCAATTAAGTTTGAATTTTTAACAGATAAAAATCTGATTATTAATTGCATGATTATTAAATTTAAAAACGGATT
TTTAATAAAAATAAATCCGTTTGGAATTAATTTTAAAAAAGAAATTATTAATTTATCAACTAATGAATTAGTATTACAAA
TTTTATTAATACAACAATTTCTAGATCAACGTAAATTAGGCAACATTACTAATGTTATTATAAAAGGATTACAGGATAGC
TTATTAAATATGGACGTTGTATCAAACTTCATTAATATTATTAATAATGAAAATGGTTTAAATATTGGAAAAAGGAAAAT
TGCAGTTTGAACAAGTGGTATAAATGTTGATTTGATTAAATGAGGACAATTACAAAATCAAATAGAATTGATTATTAGTA
TGAATGCTTCAAACTCATTAATTTATCAAAAAATAATGTTGAAGAAACTAAATCAAAATTGATCTTTTTTAAAATTAATT
GAGCAAATTAAGGCATATACAAAGATTACAAATAATCGCGTTGTATTAGAATATTTATTAATTGATAAAATTAATGATGA
TTTAAATTATGCTAATGAATTAGTAGAATTATTAAAAAACATCTTATGTTATGTGCTTTTAATTCCTTATAATTTAAATA
ATTATCGAACTAGTGCTAATTTAGATGATTTTTTTAATATTTTATCTGTTAATAAAATACGAATTAGTAAGCGTATACGA
AAAAGTAACGATTTAGATATTAGTTTTAAACAATTGAAAATTAAGGAGTAA

Upstream 100 bases:

>100_bases
AAAGCTACTCATGAAATAACAATAAAAGATTATTATCAATATAATATTATTAATGATAATCCAGATCATGCAGCAAACCA
GTTGGCGGAGATTATTCTAA

Downstream 100 bases:

>100_bases
AAAATGAATTTTGGGTTTATTAGTGATATTGGCTCACAACGCAAACATAATGATGATTGTGCATTGGTAATTCAAAATCA
ATATCAACAAACTTTATTAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNS
DGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDS
LLNMDVVSNFINIINNENGLNIGKRKIAVWTSGINVDLIKWGQLQNQIELIISMNASNSLIYQKIMLKKLNQNWSFLKLI
EQIKAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIR
KSNDLDISFKQLKIKE

Sequences:

>Translated_336_residues
MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNS
DGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDS
LLNMDVVSNFINIINNENGLNIGKRKIAV*TSGINVDLIK*GQLQNQIELIISMNASNSLIYQKIMLKKLNQN*SFLKLI
EQIKAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIR
KSNDLDISFKQLKIKE
>Mature_336_residues
MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVIILKENFSNNLLKINSYQSNS
DGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKKEIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDS
LLNMDVVSNFINIINNENGLNIGKRKIAV*TSGINVDLIK*GQLQNQIELIISMNASNSLIYQKIMLKKLNQN*SFLKLI
EQIKAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDDFFNILSVNKIRISKRIR
KSNDLDISFKQLKIKE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=329, Percent_Identity=27.6595744680851, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 38688; Mature: 38688

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEE
ILKENFSNNLLKINSYQSNSDGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKK
EEEECCCCCEEEEECCCCCCCCCEEEEEEECCCEEEEEEEEEECCCEEEEEECCCCCHHH
EIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDSLLNMDVVSNFINIINNENGL
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NIGKRKIAVTSGINVDLIKGQLQNQIELIISMNASNSLIYQKIMLKKLNQNSFLKLIEQI
CCCCEEEEEECCCCEEEEHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
KAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDD
HHHHHHCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHH
FFNILSVNKIRISKRIRKSNDLDISFKQLKIKE
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKLNNHNLKEIYSLSLNELKEELFKLGLKSFISKQIYSWIYQKRIFNFDKFSNIAKNNVI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEE
ILKENFSNNLLKINSYQSNSDGSIKFEFLTDKNLIINCMIIKFKNGFLIKINPFGINFKK
EEEECCCCCEEEEECCCCCCCCCEEEEEEECCCEEEEEEEEEECCCEEEEEECCCCCHHH
EIINLSTNELVLQILLIQQFLDQRKLGNITNVIIKGLQDSLLNMDVVSNFINIINNENGL
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NIGKRKIAVTSGINVDLIKGQLQNQIELIISMNASNSLIYQKIMLKKLNQNSFLKLIEQI
CCCCEEEEEECCCCEEEEHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
KAYTKITNNRVVLEYLLIDKINDDLNYANELVELLKNILCYVLLIPYNLNNYRTSANLDD
HHHHHHCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHH
FFNILSVNKIRISKRIRKSNDLDISFKQLKIKE
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12466555 [H]