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The map label for this gene is nemR [H]

Identifier: 170683234

GI number: 170683234

Start: 1536234

End: 1536833

Strand: Reverse

Name: nemR [H]

Synonym: EcSMS35_1549

Alternate gene names: 170683234

Gene position: 1536833-1536234 (Counterclockwise)

Preceding gene: 170682942

Following gene: 170683580

Centisome position: 30.32

GC content: 52.67

Gene sequence:

>600_bases
ATGAACAAACACACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCAC
CGGGATGGGGCTAAGCGAACTCCTAAAAACTGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAG
CGTTTGGCGTTGCCATGCTTGAGCGCCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGT
AACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCT
GACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGTAGCGCGATGGATAAAGGCGCTCGCGGCGTGA
TCGCCCTGCTCTCGCAGGCGCTGGAAAATGGCCGTGATAGCCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCA
CAAGTGCTTTACGCACTGTGGCTGGGTGCGAATCTGCAGGCCAAAATTTCGCGCAATTACGAGCCACTGGAAAACGCGCT
GGCACATGTAAAAACCATTATTGCGACGCCTGCCGTTTAG

Upstream 100 bases:

>100_bases
CGCCCTCCTCAGATAAGATTATTACCACTATTGAAGCTGTTAATGCCCAAAGTAGCAACTTTGCTTGCACTAGACCGACT
GGTCTACTACACTCCAACGC

Downstream 100 bases:

>100_bases
CAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGAT
CACGGCGGCAAACCGTATTT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGSFYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEG
NYRDRILAYYQQTLNQFCQHGTISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV

Sequences:

>Translated_199_residues
MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGSFYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEG
NYRDRILAYYQQTLNQFCQHGTISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV
>Mature_199_residues
MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGSFYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEG
NYRDRILAYYQQTLNQFCQHGTISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=199, Percent_Identity=97.4874371859297, Blast_Score=405, Evalue=1e-115,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22265; Mature: 22265

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGSFYHYFRSKEAFGVAML
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHH
ERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGTISGCLTVKLSAEVCDLSE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
DMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQAQVLYALWLGANLQAKISRNY
HHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCC
EPLENALAHVKTIIATPAV
CHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGSFYHYFRSKEAFGVAML
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHH
ERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGTISGCLTVKLSAEVCDLSE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
DMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQAQVLYALWLGANLQAKISRNY
HHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCC
EPLENALAHVKTIIATPAV
CHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]