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The map label for this gene is speG [H]

Identifier: 170682991

GI number: 170682991

Start: 1607084

End: 1607644

Strand: Reverse

Name: speG [H]

Synonym: EcSMS35_1616

Alternate gene names: 170682991

Gene position: 1607644-1607084 (Counterclockwise)

Preceding gene: 170680551

Following gene: 170681546

Centisome position: 31.72

GC content: 47.06

Gene sequence:

>561_bases
ATGCCAAGCGCCCACAGTGTTAAGCTACGCCCGCTGGAGCGTGAAGATTTACGCTATGTACATCAACTCGACAATAACGC
CAGTGTGATGCGTTACTGGTTTGAGGAACCCTACGAAGCCTTTGTTGAACTCTCTGATCTGTACGATAAGCATATTCACG
ATCAGAGCGAACGGCGCTTTGTGGTGGAATGTGACGGCGAAAAAGCCGGTCTGGTGGAGCTGGTGGAAATTAACCATGTT
CATCGCCGCGCAGAATTTCAGATAATTATCTCCCCGGAATATCAGGGGAAAGGTCTGGCAACGCGTGCCGCAAAATTAGC
AATGGACTATGGCTTCACCGTTCTCAATCTCTATAAGCTGTATCTGATCGTCGATAAAGAGAATGAAAAAGCGATTCACA
TTTACCGCAAGCTTGGCTTTACGGTTGAAGGTGAATTGATGCATGAGTTCTTTATTAATGGTCAATATCGGAATGCCATT
CGTATGTGCATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATA
G

Upstream 100 bases:

>100_bases
GCCTACTGGGAGCCAAATACTTTGCGCTGCCTGGACCGTCGAACTGGTCGCGTTATTACCCCCTAACCTGTTATTGATTT
AAGGAATGTAAGGACACGTT

Downstream 100 bases:

>100_bases
CATTAATAATAATCGATAGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAG
CTGACAGTCTACCGGATTGG

Product: diamine N-acetyltransferase

Products: NA

Alternate protein names: Diamine acetyltransferase; SAT [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHV
HRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
RMCIFQHQYLAEHKTPGQTLLKPTAQ

Sequences:

>Translated_186_residues
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHV
HRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
RMCIFQHQYLAEHKTPGQTLLKPTAQ
>Mature_185_residues
PSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVH
RRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAIR
MCIFQHQYLAEHKTPGQTLLKPTAQ

Specific function: Regulates polyamine concentration by their degradation. Acts on spermidine, forming N(1)- and N(8)-acetylspermidine [H]

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787867, Length=186, Percent_Identity=99.4623655913979, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.57 [H]

Molecular weight: Translated: 21901; Mature: 21770

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRF
CCCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEE
VVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKL
EEEECCCCCCEEEEEEHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHEEEEE
YLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTL
EEEEECCCCCEEEEEEHHCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LKPTAQ
CCCCCC
>Mature Secondary Structure 
PSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVELSDLYDKHIHDQSERRF
CCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEE
VVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKL
EEEECCCCCCEEEEEEHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHHEEEEE
YLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAIRMCIFQHQYLAEHKTPGQTL
EEEEECCCCCEEEEEEHHCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LKPTAQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]