Click here to switch to the map view.

The map label for this gene is yhjJ [H]

Identifier: 170682195

GI number: 170682195

Start: 3904983

End: 3906479

Strand: Reverse

Name: yhjJ [H]

Synonym: EcSMS35_3835

Alternate gene names: 170682195

Gene position: 3906479-3904983 (Counterclockwise)

Preceding gene: 170682606

Following gene: 170681097

Centisome position: 77.08

GC content: 54.04

Gene sequence:

>1497_bases
ATGCAGGGCACAAAAATTCGACTTTTAGCGGGCGGTTTGCTGATGATGGCCACTGCTGGCTATGTGCAGGCAGATGCGCT
CCAGCCTGATCCAGCATGGCAACAGGGGACGCTTTCCAACGGTTTACAGTGGCAAGTGCTGACTACACCCCAGCGTCCCA
GCGATCGTGTTGAAATTCGCCTGCTGGTTAATACCGGTTCGCTCGCCGAAAGTACACAACAGAGCGGTTACAGTCACGCC
ATCCCTCGTATTGCGCTAACGCAAAGCGGTGGCCTTGACGCAGCGCAGGCGCGTTCATTGTGGCAGCAGGGGATCGACCC
TAAACGCCCGATGCCGCCGGTAATTGTCTCTTATGACACCACGCTGTTTAACCTGAGTTTGCCCAATAACCGTAACGACT
TGCTGAAAGAAGCGCTCTCTTATCTGGCAAATGCCACTGGCAAACTGACTATCACGCCAGAAACCATCAACCACGCGCTG
CAAAGTCAGGACATGGTGGCAACCTGGCCTGCCGATACTAAAGAGGGCTGGTGGCGTTATCGTCTGAAAGGATCAACCTT
GTTAGGTCACGATCCTGCCGATCCGCTGAAACAACCCGTTGAAGCGGAAAAGATTAAAGATTTCTATCAGAAATGGTACA
CCCCGGATGCAATGACGCTGCTGGTGGTGGGAAACGTGGATGCGCGCTCGGTCGTCGACCAAATCAATAAAACGTTTGGC
GAACTGAAAGGCAAACGTGAAACACCGGCTCCGGTGCCGACGCTTTCTCCGCTGCGTGCGGAAGCGGTGAGTATTATGAC
CGACGCGGTGCGTCAGGACCGGTTATCTATCATGTGGGATACGCCGTGGCAGCCGATTCGTGAATCAGCCGCACTGCTGC
GCTACTGGCGTGCGGACCTGGCCCGTGAGGCGCTGTTCTGGCACGTTCAGCAAGCGTTAAGCGCCAGTAACAACAAAGAA
ATCGGTCTTGGATTTGATTGCCGTGTGCTGTATCTGCGTGCGCAGTGTGCCATCAACATCGAATCACCAAACGACAAGCT
GAACAGCAACCTTAATCTGGTGGCGCGTGAACTGGCAAAGGTTCGCGATAAAGGTCTGCCGGAAGAAGAGTTCAATGCGT
TAGTGGCGCAAAAGAAACTGGAGCTGCAGAAACTGTTTGCCGCCTATGCGCGAGCTGATACCGATATTCTGATGGGGCAG
CGGATGCGTTCGTTGCAAAATCAGGTCGTCGATATCGCGCCGGAGCAATATCAGAAACTGCGTCAGGATTTCCTTAACAG
CCTGACGGTGGAGATGTTAAATCAGGATCTGCGTCAGCAGTTGTCGAATGATATGGCGTTAATACTGCTGCAGCCGAAAG
GCGAGCCGGAATTTAACATGAAAGCGTTGCAGGCGGCCTGGGATCAAATCATGGCCCCATCGACTGCGGCTGCCGCCACA
TCTGTCGCCACGGATGACGTACATCCTGAAGTGACGGATATTCCACCCGCACAGTGA

Upstream 100 bases:

>100_bases
TTCATGTTCTTGCGGTCTAACACGAAGTGTTTTTAACGTCATTTAGACTGACCGACAACGGGCAGTTTTTTATTACCAGG
ATAGTTGATCAGGGGTTCAC

Downstream 100 bases:

>100_bases
TAAATAACCCGGCAGCGTTTCTTCTGCGCTGCCGGGATTAACGGTAATTAATAAGTTACCGTGATATACCCGCAGAGATT
TTTCCCGGCACGGCGGTAGC

Product: M16 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHA
IPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNNKE
IGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQ
RMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAAWDQIMAPSTAAAAT
SVATDDVHPEVTDIPPAQ

Sequences:

>Translated_498_residues
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHA
IPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNNKE
IGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQ
RMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAAWDQIMAPSTAAAAT
SVATDDVHPEVTDIPPAQ
>Mature_498_residues
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHA
IPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNNKE
IGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQ
RMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAAWDQIMAPSTAAAAT
SVATDDVHPEVTDIPPAQ

Specific function: Unknown

COG id: COG0612

COG function: function code R; Predicted Zn-dependent peptidases

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M16 family [H]

Homologues:

Organism=Escherichia coli, GI1789946, Length=498, Percent_Identity=99.1967871485944, Blast_Score=1010, Evalue=0.0,
Organism=Escherichia coli, GI1787770, Length=450, Percent_Identity=20.8888888888889, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011249
- InterPro:   IPR011237
- InterPro:   IPR011765
- InterPro:   IPR007863 [H]

Pfam domain/function: PF00675 Peptidase_M16; PF05193 Peptidase_M16_C [H]

EC number: NA

Molecular weight: Translated: 55511; Mature: 55511

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIR
CCCCEEEEECCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
LLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDT
EEEECCCHHHHHHHCCCHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECC
TLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHALQSQDMVATWPADTKEGWWRY
EEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCEEEECCCCCCCCCEEE
RLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
EEECCEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
AREALFWHVQQALSASNNKEIGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAK
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHCCCHHHHHHHHHH
VRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQRMRSLQNQVVDIAPEQYQKL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAAWDQIMAPSTAAAAT
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SVATDDVHPEVTDIPPAQ
HCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQWQVLTTPQRPSDRVEIR
CCCCEEEEECCCHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
LLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDT
EEEECCCHHHHHHHCCCHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECC
TLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHALQSQDMVATWPADTKEGWWRY
EEEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCEEEECCCCCCCCCEEE
RLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFG
EEECCEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHH
ELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
AREALFWHVQQALSASNNKEIGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAK
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHCCCHHHHHHHHHH
VRDKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQRMRSLQNQVVDIAPEQYQKL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
RQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAAWDQIMAPSTAAAAT
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SVATDDVHPEVTDIPPAQ
HCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 9298646 [H]