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The map label for this gene is fba [H]

Identifier: 170682060

GI number: 170682060

Start: 4071740

End: 4072600

Strand: Reverse

Name: fba [H]

Synonym: EcSMS35_3993

Alternate gene names: 170682060

Gene position: 4072600-4071740 (Counterclockwise)

Preceding gene: 170683929

Following gene: 170680800

Centisome position: 80.35

GC content: 52.38

Gene sequence:

>861_bases
ATGTTAGTTTCGATGAAAGAATTACTGCAACCTACCCGCCAGCACGGCTTTGCCATTGGCGCATTTAATGTGGCGGATAA
CTGTTTCCTTCGCGCAGTCGTGGATGAAGCAGAAGCGACAAATACTCCGGCGATTATTGCCATCCATCCCAGCGAACATG
ATTTTGTGGGCGATGCCTTTTTCGCTCACGTTCGCGAAATTACCCAGCGTAGTCCGGTCCCGTTTGTACTGCACCTCGAT
CATGGTGCCTCTGTAGAGCATGTTTTGCGTGCGATTCGCTGCGGATTTACCTCAGTAATGATCGACGGTTCGCTGCTGCC
GTATGAAGAAAACGTGGCGCTGACCGCAGAAGTGGTTCGCCTCGCTCACGCGGTTGGAGTGTCGGTTGAGGGTGAACTGG
GAACCATCGGACAAACAGGAACGTCGGTAGAAGGTGGTGTTTCACAAGTGACCTACACAGATCCGGCGCAGGCCGCCGAT
TTTATTGCTCGTACAGGTGCCGACACTCTGGCTGTGGCGATTGGTACAGCGCATGGCATCTATCCAAAAGGGATGCAGCC
GAAGCTGCAAATGGACATTTTGCGCGACATTGCCGGACGTATTGATATTCCGCTGGTGCTACACGGTGGTTCGGCAAACC
CGGACGCGGAGATTGCTGAATCAGTTACGCTCGGCGTCGGCAAAATCAATATCTCCAGCGATATGAAATACGCCTATTTC
CAGAAAGTGCGTGAAATTCTCGCTAAAGAGAGCTGGTGGGACCCGAACGTCATCTATCCGGATGCGATTAACGCGGCGCG
GGAAGTCATTCGCCAGAAAATGAAACTGTTCGGTTCGATCGGTAAAGCGTCACTGTACTAA

Upstream 100 bases:

>100_bases
GCTCTATCGGCAAAGCCTGATTCATTTTCTGTCATGGCCTTTATGCCAGTGCAATAACGCACCGGCAGGGCGGACTACAC
GCTGAAACTGGAGATTTAAC

Downstream 100 bases:

>100_bases
GCCAGAAGGGCGGGGCAACCCGCCTTTTACGCAACAAGGAACGAGATATGCACTATCTGGGGTTGGATATTGGTGGGACC
AAAATCGCCGCCGTCGTCAT

Product: hypothetical protein

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPAIIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLD
HGASVEHVLRAIRCGFTSVMIDGSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEIAESVTLGVGKINISSDMKYAYF
QKVREILAKESWWDPNVIYPDAINAAREVIRQKMKLFGSIGKASLY

Sequences:

>Translated_286_residues
MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPAIIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLD
HGASVEHVLRAIRCGFTSVMIDGSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEIAESVTLGVGKINISSDMKYAYF
QKVREILAKESWWDPNVIYPDAINAAREVIRQKMKLFGSIGKASLY
>Mature_286_residues
MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPAIIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLD
HGASVEHVLRAIRCGFTSVMIDGSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEIAESVTLGVGKINISSDMKYAYF
QKVREILAKESWWDPNVIYPDAINAAREVIRQKMKLFGSIGKASLY

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=285, Percent_Identity=34.7368421052632, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788072, Length=288, Percent_Identity=36.8055555555556, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI87082041, Length=284, Percent_Identity=35.2112676056338, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789293, Length=284, Percent_Identity=24.2957746478873, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322790, Length=294, Percent_Identity=24.8299319727891, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 30805; Mature: 30805

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPAIIAIHPSEHDFVGDAF
CCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
FAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMIDGSLLPYEENVALTAEVVR
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCCCCCCCCCHHHHHHHH
LAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAADFIARTGADTLAVAIGTAHGI
HHHHHCCEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCC
YPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEIAESVTLGVGKINISSDMKYAYF
CCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHEEEEEEEECCCCCHHHHH
QKVREILAKESWWDPNVIYPDAINAAREVIRQKMKLFGSIGKASLY
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPAIIAIHPSEHDFVGDAF
CCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
FAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMIDGSLLPYEENVALTAEVVR
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCHHEEECCCCCCCCCCCHHHHHHHH
LAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAADFIARTGADTLAVAIGTAHGI
HHHHHCCEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCCC
YPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEIAESVTLGVGKINISSDMKYAYF
CCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHEEEEEEEECCCCCHHHHH
QKVREILAKESWWDPNVIYPDAINAAREVIRQKMKLFGSIGKASLY
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA