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The map label for this gene is rfaP [H]

Identifier: 170681792

GI number: 170681792

Start: 4043179

End: 4043976

Strand: Reverse

Name: rfaP [H]

Synonym: EcSMS35_3965

Alternate gene names: 170681792

Gene position: 4043976-4043179 (Counterclockwise)

Preceding gene: 170682059

Following gene: 170681283

Centisome position: 79.79

GC content: 47.24

Gene sequence:

>798_bases
ATGGTTGAACTTAAAGAGCCGTTTGCCACGTTATGGCGCGGTAAAGATCCTTTTGAGGAAGTTAAAACCTTGCAGGGTGA
GGTATTTCGTGAACTGGAAACTCGCCGCACTCTGCGCTTTGAAATGGCGGGCAAAAGCTATTTTCTCAAATGGCATCGCG
GCACGACCCTGAAAGAGATAATCAAAAATTTACTCTCATTGCGGATGCCAGTATTAGGCGCAGACCGCGAATGGAATGCG
ATTCATCGACTGCGGGATGTCGGCGTTGATACTATGTATGGGGTGGCATTCGGCGAAAAAGGCATTAATCCGCTGACCAG
AACCTCGTTTATTATTACCGAAGATCTGACACCAACCATCAGTCTGGAAGATTACTGTGCTGACTGGGCGACCAACCCAC
CAGATGTTCGCGTAAAGCGTATGCTTATTAAGCGTGTCGCGACGATGGTGCGCGATATGCATGCTGCGGGCATTAACCAC
CGTGACTGTTATATCTGTCATTTCCTGCTGCACTTGCCTTTTTCCGGTAAGGAAGAGGAGTTAAAAATTTCAGTAATTGA
CCTGCACCGGGCGCAGCTTCGCACGCGCGTTCCATGTCGTTGGCGCGATAAAGATCTTATTGGACTTTATTTTTCTTCGA
TGAATATTGGCCTGACTCAACGGGATATCTGGCGGTTTATGAAAGTGTATTTTGTCGCCCCGCTTAAAGACATTCTCAAG
CAGGAACAAGGGCTGCTGTCGCAAGCAGAAGAAAAAGCCACAAAAATCAGGGAAAGAACGATTCGAAAATCGTTGTAA

Upstream 100 bases:

>100_bases
TTGCGCCAGGCCTGGGCGGAAAATGCGCGACATTATGCTGATACACAAGATTTATACAGTCTGCCAGAGAAAGCGGCGGA
CATCATAACGGGTGGTCTGG

Downstream 100 bases:

>100_bases
TTTCCGGAGAGAAATATGAGTGCCCACTATTTTAATCCACAAGAGATGATCAATAAGACAATCATCTTCGATGAAAGGCC
AGAGGCGTCAGTGGCATCAT

Product: lipopolysaccharide core biosynthesis protein RfaP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNA
IHRLRDVGVDTMYGVAFGEKGINPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPCRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFVAPLKDILK
QEQGLLSQAEEKATKIRERTIRKSL

Sequences:

>Translated_265_residues
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNA
IHRLRDVGVDTMYGVAFGEKGINPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPCRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFVAPLKDILK
QEQGLLSQAEEKATKIRERTIRKSL
>Mature_265_residues
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNA
IHRLRDVGVDTMYGVAFGEKGINPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPCRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFVAPLKDILK
QEQGLLSQAEEKATKIRERTIRKSL

Specific function: Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. KdkA/rfaP family [H]

Homologues:

Organism=Escherichia coli, GI1790060, Length=265, Percent_Identity=83.7735849056604, Blast_Score=467, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017172
- InterPro:   IPR010440 [H]

Pfam domain/function: PF06293 Kdo [H]

EC number: 2.7.-.-

Molecular weight: Translated: 31067; Mature: 31067

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEI
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCHHHHH
IKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGINPLTRTSFIITEDLTPTI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCEEEEEECCCCCC
SLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINHRDCYICHFLLHLPFSGKEEE
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
LKISVIDLHRAQLRTRVPCRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFVAPLKDILK
EEEEEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QEQGLLSQAEEKATKIRERTIRKSL
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGKSYFLKWHRGTTLKEI
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCHHHHH
IKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGINPLTRTSFIITEDLTPTI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCEEEEEECCCCCC
SLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINHRDCYICHFLLHLPFSGKEEE
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
LKISVIDLHRAQLRTRVPCRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFVAPLKDILK
EEEEEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QEQGLLSQAEEKATKIRERTIRKSL
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA