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The map label for this gene is mak [H]

Identifier: 170680800

GI number: 170680800

Start: 4070760

End: 4071692

Strand: Reverse

Name: mak [H]

Synonym: EcSMS35_3992

Alternate gene names: 170680800

Gene position: 4071692-4070760 (Counterclockwise)

Preceding gene: 170682060

Following gene: 170680492

Centisome position: 80.34

GC content: 53.48

Gene sequence:

>933_bases
ATGCACTATCTGGGGTTGGATATTGGTGGGACCAAAATCGCCGCCGTCGTCATGGATGCGCATGGCTGGGAGATTCGCCG
TTACCGCTGCCCGACGCAAAAGTCGACATATCAACAATTTGTCTCATGCGTTGTGGCGCTTATCGAGCAGATTAGGCGGG
ACGTTCAACGACCGATGCTGACAGGGATCGCCTTACCCGGCAGTATCTCGCCACTCACTGGCCTGATTAAAAACGCGAAT
ATTCAGGTGATTAACGGTCATGCGTTACAGGCTGATTTGCAGCAATTGCTTGGGCAACCGGTGGTGATAGCCAATGATGG
TAACTGTTTCGCGCTATCAGAAGCTTGCGACGGTGCCGGGCAAGATTATGACGTGGTATTTGGTATTACGCTTGGTTCGG
GCTGCGGCGGTGGCATTGCCATCAAGCAACGACCGTTTATAGGGGCCTGGGGAAATGCTGCCGAATGCGGTCATATCACG
TTGCCAGGCTATATGGAGCAGGAAGATGGTCCATCAGTCAGTTGCTATTGCGGCAAACACAACTGCGTGGAGTCGTTTGT
TTCCGGCAGCGGTTTTAGTGAACGCTATCAACAGATGACTGGTAACTTGCTCACTCCTGCGGCGATTGTCACCCTGGCAC
AACGTGGTGATGCTTGTGCCATGCAGCAGGTGGCACGTTTTCGCCAACAGCTTGCCCGCACGCTGGCAACCATCGTTAAC
GTTGTTGACCCTGGCGTGATTGTCATCGGCGGCGGGCTTTCGAATGTGGAACTGCTTATCGCCGATCTGAACACAGAAGT
CGCTCCTCTGGTTTTCACCGACCAATTCACCACCCCCATTGTAAAAGCACTGCACGGCGACAGTAGCGGAATGCGTGGCG
CTGCCTGGCTTGCTATGCGCAACGGAGAAGCCAATGAAACGTTCACAAATTAA

Upstream 100 bases:

>100_bases
TTCGCCAGAAAATGAAACTGTTCGGTTCGATCGGTAAAGCGTCACTGTACTAAGCCAGAAGGGCGGGGCAACCCGCCTTT
TACGCAACAAGGAACGAGAT

Downstream 100 bases:

>100_bases
TTATGTTATTGATAAAGCACATGCCATTGCGCAGACCTTCCGCGTTTGTTTGCCAGAGTTCGCCTATTTTACGGTGGATG
ACTGGCTGCAGCTGGAACGG

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNAN
IQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAGQDYDVVFGITLGSGCGGGIAIKQRPFIGAWGNAAECGHIT
LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDACAMQQVARFRQQLARTLATIVN
VVDPGVIVIGGGLSNVELLIADLNTEVAPLVFTDQFTTPIVKALHGDSSGMRGAAWLAMRNGEANETFTN

Sequences:

>Translated_310_residues
MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNAN
IQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAGQDYDVVFGITLGSGCGGGIAIKQRPFIGAWGNAAECGHIT
LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDACAMQQVARFRQQLARTLATIVN
VVDPGVIVIGGGLSNVELLIADLNTEVAPLVFTDQFTTPIVKALHGDSSGMRGAAWLAMRNGEANETFTN
>Mature_310_residues
MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNAN
IQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAGQDYDVVFGITLGSGCGGGIAIKQRPFIGAWGNAAECGHIT
LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDACAMQQVARFRQQLARTLATIVN
VVDPGVIVIGGGLSNVELLIADLNTEVAPLVFTDQFTTPIVKALHGDSSGMRGAAWLAMRNGEANETFTN

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI190014632, Length=273, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4885285, Length=273, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI298566315, Length=273, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI298566325, Length=273, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI87081733, Length=297, Percent_Identity=40.7407407407407, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=36.7549668874172, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786891, Length=323, Percent_Identity=24.1486068111455, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87082230, Length=284, Percent_Identity=30.2816901408451, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787878, Length=268, Percent_Identity=25.3731343283582, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790522, Length=305, Percent_Identity=26.5573770491803, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32958; Mature: 32958

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPML
CEEEEEECCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAG
HCEECCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEEECCCCEEEEHHHCCCCC
QDYDVVFGITLGSGCGGGIAIKQRPFIGAWGNAAECGHITLPGYMEQEDGPSVSCYCGKH
CCEEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCEEECCCCCCCCCCCCEEEEECCH
NCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDACAMQQVARFRQQLARTLATIVN
HHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VVDPGVIVIGGGLSNVELLIADLNTEVAPLVFTDQFTTPIVKALHGDSSGMRGAAWLAMR
HCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEE
NGEANETFTN
CCCCCCCCCC
>Mature Secondary Structure
MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCVVALIEQIRRDVQRPML
CEEEEEECCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVVIANDGNCFALSEACDGAG
HCEECCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHCCCCEEEECCCCEEEEHHHCCCCC
QDYDVVFGITLGSGCGGGIAIKQRPFIGAWGNAAECGHITLPGYMEQEDGPSVSCYCGKH
CCEEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCEEECCCCCCCCCCCCEEEEECCH
NCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDACAMQQVARFRQQLARTLATIVN
HHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
VVDPGVIVIGGGLSNVELLIADLNTEVAPLVFTDQFTTPIVKALHGDSSGMRGAAWLAMR
HCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEE
NGEANETFTN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]