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The map label for this gene is uhpT [H]

Identifier: 170680555

GI number: 170680555

Start: 4119215

End: 4120606

Strand: Reverse

Name: uhpT [H]

Synonym: EcSMS35_4031

Alternate gene names: 170680555

Gene position: 4120606-4119215 (Counterclockwise)

Preceding gene: 170679869

Following gene: 170683707

Centisome position: 81.3

GC content: 53.09

Gene sequence:

>1392_bases
ATGCTGGCTTTCTTAAACCAGGTTCGCAAGCCGACCCTGGACCTTCCGCTCGAAGTGCGGCGCAAAATGTGGTTCAAACC
GTTCATGCAATCCTACCTGGTGGTCTTTATCGGCTACCTGACGATGTACCTGATTCGCAAGAACTTTAACATCGCGCAGA
ACGATATGATTTCGACCTACGGGTTGAGCATGACGCAGCTGGGGATGATCGGCCTGGGTTTCTCCATCACTTATGGCGTG
GGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTGTCTGCCAT
TTGTATGCTGGGCTTCAGCGCCAGTATGGGCAGCGGCTCGGTTAGCCTGTTCCTGATGATTGCCTTCTACGCCTTAAGCG
GCTTTTTCCAGAGCACCGGCGGTTCGTGCAGTTACTCCACCATCACCAAATGGACGCCGCGTCGTAAGCGCGGGACCTTC
CTCGGTTTCTGGAACATTTCTCACAACCTTGGCGGTGCAGGCGCAGCAGGTGTGGCGCTGTTCGGCGCAAATTACCTGTT
CGATGGTCATGTTATCGGCATGTTTATCTTCCCGTCGATTATCGCGCTGATTGTTGGTTTTATCGGCCTGCGTTACGGCA
GCGACTCCCCGGAATCTTATGGCCTCGGCAAAGCTGAAGAGCTGTTCGGCGAGGAGATCAGTGAAGAGGATAAAGAGACA
GAATCTACCGATATGACCAAGTGGCAGATCTTTGTTGAGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTTGC
CAACATTTTCCTCTATGTGGTGCGTATTGGTATCGACCAGTGGTCAACCGTGTATGCGTTCCAGGAACTGAAACTCTCTA
AAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGGGCTGGCTCTCTGAC
CTGGCGAACGGTCGCCGTGGCCTGGTGGCCTGTATCGCGCTGGCGCTGATTATCGCCACGCTCGGTGTGTATCAACACGC
CAGCAACCAATATATCTACCTGGCTTCTCTCTTTGCGTTGGGTTTCCTGGTCTTTGGCCCGCAATTGTTGATTGGTGTAG
CTGCTGTTGGCTTTGTACCTAAAAAAGCGATTGGCGCTGCCGATGGTATTAAAGGCACCTTCGCTTACCTGATTGGTGAC
AGCTTTGCCAAGTTAGGTCTGGGAATGATTGCTGATGGGACGCCGGTATTTGGCCTTACCGGCTGGGCAGGCACCTTCGC
CGCGCTGGATATCGCCGCGATTGGTTGTATCTGCCTGATGGCGATAGTGGCGGTGATGGAAGAACGCAAAATCCGCCGCG
AGAAAAAAATTCAGCAGTTGACAGTGGCATAA

Upstream 100 bases:

>100_bases
AGAAATTTTCCAGGTTTTGCCTGGACGCTATCTCAGGCCTGATTTGCTACTGATTTTTACAATGCATGCCTCACGCAGGT
ATTCATTTCAGGAGTAACCC

Downstream 100 bases:

>100_bases
ACGTAACTGGTGACTTTTGCCCGGCATGACGCCGGGCTTTTTTTTATTATTCCGTGACTTCCAGCGTAGTGAAGGCAAAC
TTCTCGCCATCAAACAGCCC

Product: sugar phosphate antiporter

Products: Proton [Cytoplasm]; glucose-6-phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYLIRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGV
GKTLVSYYADGKNTKQFLPFMLILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPESYGLGKAEELFGEEISEEDKET
ESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRIGIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSD
LANGRRGLVACIALALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKGTFAYLIGD
SFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERKIRREKKIQQLTVA

Sequences:

>Translated_463_residues
MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYLIRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGV
GKTLVSYYADGKNTKQFLPFMLILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPESYGLGKAEELFGEEISEEDKET
ESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRIGIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSD
LANGRRGLVACIALALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKGTFAYLIGD
SFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERKIRREKKIQQLTVA
>Mature_463_residues
MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYLIRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGV
GKTLVSYYADGKNTKQFLPFMLILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPESYGLGKAEELFGEEISEEDKET
ESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRIGIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSD
LANGRRGLVACIALALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKGTFAYLIGD
SFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERKIRREKKIQQLTVA

Specific function: Transport protein for sugar phosphate uptake [H]

COG id: COG2271

COG function: function code G; Sugar phosphate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family [H]

Homologues:

Organism=Homo sapiens, GI256219598, Length=436, Percent_Identity=23.6238532110092, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI4503847, Length=436, Percent_Identity=23.6238532110092, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI256219483, Length=436, Percent_Identity=23.6238532110092, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367259, Length=463, Percent_Identity=99.7840172786177, Blast_Score=932, Evalue=0.0,
Organism=Escherichia coli, GI1788573, Length=457, Percent_Identity=31.7286652078775, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87082310, Length=463, Percent_Identity=33.0453563714903, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17542316, Length=483, Percent_Identity=22.5672877846791, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656866, Length=496, Percent_Identity=21.3709677419355, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550478, Length=496, Percent_Identity=21.3709677419355, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24656861, Length=493, Percent_Identity=21.501014198783, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000849
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR021159 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50606; Mature: 50606

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS ; PS00942 GLPT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; glucose-6-phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + glucose-6-phosphate [Periplasm] = Proton [Cytoplasm] + glucose-6-phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]