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The map label for this gene is yeaI [H]

Identifier: 170680353

GI number: 170680353

Start: 1388450

End: 1389784

Strand: Reverse

Name: yeaI [H]

Synonym: EcSMS35_1406

Alternate gene names: 170680353

Gene position: 1389784-1388450 (Counterclockwise)

Preceding gene: 170679771

Following gene: 170683497

Centisome position: 27.42

GC content: 39.18

Gene sequence:

>1335_bases
ATGGGTACACAAAAATTAAAAGCTCAAGGCTTTTTTGTTTTCAGCTTATTGCTGACGTTAATTTTATTTTGCATTACTAC
TTTATATAACGAAAACACGAATGTAAAACTCATCCCACAGATGAATTACCTGATGGTTGTTGTGGCTTTGTTTTTCCTTA
ACGCCGTCATTTTTCTTTTCATGTTAATGAAGTATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCA
TTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATCAACGGCAGTACACTGATCCAGAC
AAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTCATTTGTTTAACCTCGCTGGCGCTCTTTTGTT
ATGGGAAAGACAACATCCTTGACAACAATAAGAAAAAAACTGGCATTCTGTTGCTGGCGCTGATTCCTTTTTTAGTTTTT
CCCCTTCTGGCACACAATCTGAGTAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATAC
TGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTTTGTGGGCATTTTTACTGTTCTTTATTATCATGCGTACAC
GATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTG
GATGAGTATAATTACACCATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGAT
TTATAACATTTTTCAGGAACTGCAACTCTCCAGCAAACTGGCAGTTCATGATGTGCTGACCAATATTTATAATCGGCGCT
ACTTTTTCAACAGCGTAGAGTCATTATTGTCGCGACCTGTTGTTAAGGACTTTTGTGTCATGCTGGTTGATATTAATCAG
TTCAAACGCATCAATGCCCAATGGGGACATCGTGTGGGTGATAAAGTGCTGGTATCAATTGTCGATATTATCCAGCAAAG
CATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGG
CAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAA
ATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCATAATATCTCGCTTGTCATGACGGAAGCAGATAAAGC
CTTACGCGAAGCGAAAAGCGAGGGGGGCAACAAAGTGATTATTCATCATATTTAA

Upstream 100 bases:

>100_bases
TTATTTTTTATCGTCAACGAAAATTGATCCTGGTCAAAACTATATATCTCTGCCATCAATAAAATCCAGCACTCACATTG
CTCTCCTTTTTATGGCTTCT

Downstream 100 bases:

>100_bases
GTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCC
CAGCGGCGCAGAGATGAGTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MGTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLA
FLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVF
PLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
FKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
MTISIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI

Sequences:

>Translated_444_residues
MGTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLA
FLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVF
PLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQ
FKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
MTISIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI
>Mature_443_residues
GTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFP
LLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLD
EYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQF
KRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQM
TISIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1788085, Length=444, Percent_Identity=99.3243243243243, Blast_Score=897, Evalue=0.0,
Organism=Escherichia coli, GI1787262, Length=449, Percent_Identity=37.1937639198218, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI87082007, Length=227, Percent_Identity=29.9559471365639, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=29.1208791208791, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=30.939226519337, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=28.9940828402367, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786584, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=29.2817679558011, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 50731; Mature: 50600

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLF
CCCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
MLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHH
LSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVD
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
ECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVE
HCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGE
HHHHCCHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCH
VFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISLV
HHHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEE
MTEADKALREAKSEGGNKVIIHHI
EEHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
GTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLF
CCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
MLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQ
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHH
LSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVD
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
ECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVE
HCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGE
HHHHCCHHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCH
VFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISLV
HHHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEE
MTEADKALREAKSEGGNKVIIHHI
EEHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]