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The map label for this gene is yeaP [H]

Identifier: 170679654

GI number: 170679654

Start: 1381570

End: 1382595

Strand: Reverse

Name: yeaP [H]

Synonym: EcSMS35_1397

Alternate gene names: 170679654

Gene position: 1382595-1381570 (Counterclockwise)

Preceding gene: 170681477

Following gene: 170683641

Centisome position: 27.28

GC content: 48.73

Gene sequence:

>1026_bases
GTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGCCTGGTCCGACAGCTTCT
GGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATAA
TGTTTGCCCGTAACAGCCAGAAAATGCACATCCCGGAGAATTTTACCGTCTCGTGGGATTATTCGTTATGCAAACGCGCC
ATTGATGAAAACTGCTTTTTCAGCGATGAAGTCCCCGACCGTTGGGGCGACTGTATTGCGGCACGCAATCTTGGCATCAC
CACATTTCTGAGCACGCCAATTCACTTACCGGATGGATCATTCTATGGCACGCTTTGCGCCGCCAGCAGTGAGAAGCGCC
AGTGGAGTGAACGCGCGGAACAGGTTTTGCAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCGCTGGTA
GAACAGCTGCGCGAAGCCAATGCCGCACTGATAGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGC
GATTTTTGAAAATCTGACGACGCTGTTTTCTCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGACCTGG
ATAACTTCAAATTAATCAACGATCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAAT
ACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTGGTTGTTTCACTGAACAACGAGAATAC
GGATATTTCGTCGCTGCGAGAACGTATTCAACAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATC
CCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACGACCGATGCAGACAGTGCCCTGCATGCTGCCGATATCGCG
ATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGA

Upstream 100 bases:

>100_bases
AAATCATATTACGCCTTGTTTCATCTTTGTTGATGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCG
CCGTATCCTGGAGTTGTTTC

Downstream 100 bases:

>100_bases
GGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTTATTACAGCGGTC
GTATTTTTAGCATGGTTTTT

Product: GAF domain/diguanylate cyclase domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKVDVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRA
IDENCFFSDEVPDRWGDCIAARNLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLIQVGERLN
TLQQNGEVIGRLGGDEFLVVSLNNENTDISSLRERIQQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIA
MYQEKKHKQKTPFVAHPALHS

Sequences:

>Translated_341_residues
MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKVDVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRA
IDENCFFSDEVPDRWGDCIAARNLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLIQVGERLN
TLQQNGEVIGRLGGDEFLVVSLNNENTDISSLRERIQQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIA
MYQEKKHKQKTPFVAHPALHS
>Mature_340_residues
SDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKVDVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAI
DENCFFSDEVPDRWGDCIAARNLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALVE
QLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLIQVGERLNT
LQQNGEVIGRLGGDEFLVVSLNNENTDISSLRERIQQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAM
YQEKKHKQKTPFVAHPALHS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger (Probable). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081977, Length=341, Percent_Identity=99.4134897360704, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=32.2033898305085, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=33.5365853658537, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=29.6969696969697, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=29.3478260869565, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=101, Percent_Identity=41.5841584158416, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=30, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003018 [H]

Pfam domain/function: PF01590 GAF; PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38550; Mature: 38419

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKVDVEARLQHIMFARNSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCCC
KMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAARNLGITTFLSTPIHLPDGS
CEECCCCCEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEECCCCC
FYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALVEQLREANAALIAQSYTDSLT
CEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLIQVGERLN
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEHHHCCCCCCCEEEEEECHHHH
TLQQNGEVIGRLGGDEFLVVSLNNENTDISSLRERIQQQIRGEYHLGDVDLYYPGASLGI
HHHHCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEE
VEVDPETTDADSALHAADIAMYQEKKHKQKTPFVAHPALHS
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKVDVEARLQHIMFARNSQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCCC
KMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAARNLGITTFLSTPIHLPDGS
CEECCCCCEEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEECCCCC
FYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALVEQLREANAALIAQSYTDSLT
CEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
GLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKLINDRFGHNSGDLFLIQVGERLN
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEHHHCCCCCCCEEEEEECHHHH
TLQQNGEVIGRLGGDEFLVVSLNNENTDISSLRERIQQQIRGEYHLGDVDLYYPGASLGI
HHHHCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEE
VEVDPETTDADSALHAADIAMYQEKKHKQKTPFVAHPALHS
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]