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The map label for this gene is wcaG [C]

Identifier: 170077469

GI number: 170077469

Start: 872160

End: 873362

Strand: Reverse

Name: wcaG [C]

Synonym: SYNPCC7002_A0847

Alternate gene names: 170077469

Gene position: 873362-872160 (Counterclockwise)

Preceding gene: 170077470

Following gene: 170077467

Centisome position: 29.03

GC content: 48.63

Gene sequence:

>1203_bases
ATGAAAATTATTGTTCTGGGTGGAGATGGGTTTTGCGGCTGGCCCACAGCATTGCATCTGTCGAAAACAGGTCATGATGT
TGTTATTGTTGATAACCTTTCCAGAAGAAATATTGATAACGAACTGGAAGTAACATCACTGACGCCGATTAGCCCAATGT
CGGTCAGGCTCAGAGCATGGAAAGAACTCACTGGCAAAACAATTCAGTTCTTCAACTTCGATATTGCCCACGAATATGAC
CAGCTTCTCAAGCTATTCGTTGACTATCAACCGGATGTGGTCGTTCACTTCGCCGAACAGCGGGCCGCGCCTTACTCCAT
GAAGTCTCCCAAGCACAAGCGTTATACCGTCGATAACAATCTAAACGGAACCAATAACGTACTTTGTGCCATTGTAGAAT
CCGGCCTTGATATCCACGTGGTACACCTCGGCACCATGGGGGTCTATGGTTACGGTACCGCTGGGATGAAGATCCCCGAA
GGCTACCTCGATGTTCAAGTGGTCACCGAAGAAGGCAAAGTGATCGAGAAGCAAATTCTGCACCCAGCAAATCCTGGCAG
CATCTATCACATGACCAAAACCCAGGATCAACTATTTTTTGCCTACTACAACAAAAATGACCAAGTCCGCGTCACTGACT
TGCACCAAGGGATTGTTTGGGGTACGAACACCGCAGAAACAGCAATGGACGAGCGGCTCATCAACCGCTTTGATTATGAT
GGCGACTATGGCACCGTGCTGAATCGCTTTTTGATGCAGGCAGCCGTAGGCCATCCGCTGACTGTCCATGGTACTGGCGG
CCAAACCCGTGCTTTTATTCATATTCAAGATACGGTGCGTTGTGTTGAACTGGCGATCAGTAATCCTCCGGCGAAAGGGG
AACGGGTCAAAATTCTCAACCAGATGACGGAAACCCACCGCGTCCGGGATCTGGCGCAGATGGTGGCGAATATCGCTGGG
GCGGAAATTCAATATTTAGAAAATCCCCGGAATGAAGCAGCAGAAAACGAACTGTATGTAGATAATCAGTGTTTCCTCGA
TATGGGCCTAGAACCGACCAAGCTCGATAAGGGATTGATGTTAGAGGTCACAGAAATCGCGAAGAAATATGTGGATCGCT
GCGATCGCTCCCGAATCCTCTGTACTTCTAAGTGGAACCAAAATGCGACCGCAGAACCGCTGAAAGTCCATGCAAAGGTC
TAG

Upstream 100 bases:

>100_bases
ATGTATGGCGTACCATGAACCCCAAAAAATTTTCCGGGAGTACACACCATGCACATTACTTTTTAAAGCACCAAAATAAA
AAACCTAAGGAGTTGAATCC

Downstream 100 bases:

>100_bases
ATAGGTTTAATTAAATTTTGGCGTAATGGGGGGCGATCGCCCCTCTTTTTTGTGTTGACTTGAATTGTTGGCTAGTATAG
TGGCGAAGTGGTGAGATGCG

Product: NAD dependent epimerase/dehydratase family protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase; UDP Sulfoquinovose Synthase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKIIVLGGDGFCGWPTALHLSKTGHDVVIVDNLSRRNIDNELEVTSLTPISPMSVRLRAWKELTGKTIQFFNFDIAHEYD
QLLKLFVDYQPDVVVHFAEQRAAPYSMKSPKHKRYTVDNNLNGTNNVLCAIVESGLDIHVVHLGTMGVYGYGTAGMKIPE
GYLDVQVVTEEGKVIEKQILHPANPGSIYHMTKTQDQLFFAYYNKNDQVRVTDLHQGIVWGTNTAETAMDERLINRFDYD
GDYGTVLNRFLMQAAVGHPLTVHGTGGQTRAFIHIQDTVRCVELAISNPPAKGERVKILNQMTETHRVRDLAQMVANIAG
AEIQYLENPRNEAAENELYVDNQCFLDMGLEPTKLDKGLMLEVTEIAKKYVDRCDRSRILCTSKWNQNATAEPLKVHAKV

Sequences:

>Translated_400_residues
MKIIVLGGDGFCGWPTALHLSKTGHDVVIVDNLSRRNIDNELEVTSLTPISPMSVRLRAWKELTGKTIQFFNFDIAHEYD
QLLKLFVDYQPDVVVHFAEQRAAPYSMKSPKHKRYTVDNNLNGTNNVLCAIVESGLDIHVVHLGTMGVYGYGTAGMKIPE
GYLDVQVVTEEGKVIEKQILHPANPGSIYHMTKTQDQLFFAYYNKNDQVRVTDLHQGIVWGTNTAETAMDERLINRFDYD
GDYGTVLNRFLMQAAVGHPLTVHGTGGQTRAFIHIQDTVRCVELAISNPPAKGERVKILNQMTETHRVRDLAQMVANIAG
AEIQYLENPRNEAAENELYVDNQCFLDMGLEPTKLDKGLMLEVTEIAKKYVDRCDRSRILCTSKWNQNATAEPLKVHAKV
>Mature_400_residues
MKIIVLGGDGFCGWPTALHLSKTGHDVVIVDNLSRRNIDNELEVTSLTPISPMSVRLRAWKELTGKTIQFFNFDIAHEYD
QLLKLFVDYQPDVVVHFAEQRAAPYSMKSPKHKRYTVDNNLNGTNNVLCAIVESGLDIHVVHLGTMGVYGYGTAGMKIPE
GYLDVQVVTEEGKVIEKQILHPANPGSIYHMTKTQDQLFFAYYNKNDQVRVTDLHQGIVWGTNTAETAMDERLINRFDYD
GDYGTVLNRFLMQAAVGHPLTVHGTGGQTRAFIHIQDTVRCVELAISNPPAKGERVKILNQMTETHRVRDLAQMVANIAG
AEIQYLENPRNEAAENELYVDNQCFLDMGLEPTKLDKGLMLEVTEIAKKYVDRCDRSRILCTSKWNQNATAEPLKVHAKV

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 45017; Mature: 45017

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVLGGDGFCGWPTALHLSKTGHDVVIVDNLSRRNIDNELEVTSLTPISPMSVRLRAW
CEEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCCCCCEEEEEECCCCCHHHHHHHH
KELTGKTIQFFNFDIAHEYDQLLKLFVDYQPDVVVHFAEQRAAPYSMKSPKHKRYTVDNN
HHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCCCCEEEECCC
LNGTNNVLCAIVESGLDIHVVHLGTMGVYGYGTAGMKIPEGYLDVQVVTEEGKVIEKQIL
CCCCCCEEEEEEECCCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHC
HPANPGSIYHMTKTQDQLFFAYYNKNDQVRVTDLHQGIVWGTNTAETAMDERLINRFDYD
CCCCCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCCC
GDYGTVLNRFLMQAAVGHPLTVHGTGGQTRAFIHIQDTVRCVELAISNPPAKGERVKILN
CCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHHEEEEEEEECCCCCCCHHHHHHH
QMTETHRVRDLAQMVANIAGAEIQYLENPRNEAAENELYVDNQCFLDMGLEPTKLDKGLM
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCE
LEVTEIAKKYVDRCDRSRILCTSKWNQNATAEPLKVHAKV
EEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKIIVLGGDGFCGWPTALHLSKTGHDVVIVDNLSRRNIDNELEVTSLTPISPMSVRLRAW
CEEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCCCCCEEEEEECCCCCHHHHHHHH
KELTGKTIQFFNFDIAHEYDQLLKLFVDYQPDVVVHFAEQRAAPYSMKSPKHKRYTVDNN
HHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCCCCCEEEECCC
LNGTNNVLCAIVESGLDIHVVHLGTMGVYGYGTAGMKIPEGYLDVQVVTEEGKVIEKQIL
CCCCCCEEEEEEECCCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEEECCCCHHHHHHC
HPANPGSIYHMTKTQDQLFFAYYNKNDQVRVTDLHQGIVWGTNTAETAMDERLINRFDYD
CCCCCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCCC
GDYGTVLNRFLMQAAVGHPLTVHGTGGQTRAFIHIQDTVRCVELAISNPPAKGERVKILN
CCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEHHHEEEEEEEECCCCCCCHHHHHHH
QMTETHRVRDLAQMVANIAGAEIQYLENPRNEAAENELYVDNQCFLDMGLEPTKLDKGLM
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCE
LEVTEIAKKYVDRCDRSRILCTSKWNQNATAEPLKVHAKV
EEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA