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The map label for this gene is ada [H]

Identifier: 170026341

GI number: 170026341

Start: 4566575

End: 4567639

Strand: Reverse

Name: ada [H]

Synonym: YPK_4134

Alternate gene names: 170026341

Gene position: 4567639-4566575 (Counterclockwise)

Preceding gene: 170026343

Following gene: 170026338

Centisome position: 97.4

GC content: 52.02

Gene sequence:

>1065_bases
ATGAATAACGTAAAAGACCCTCGCTGGGCCGCGATTATCAATCGGGATAAAACCGCCGATGGTCAGTTTGTGTATGCGGT
AAAAACGACGGGCATATATTGCCGCCCCTCTTGCCCATCTCGTCGAGCCAAAGCAGAACACATTGAATTCTTTATCGATA
ATACGGCAGCAGAGCAAGCCGGTTATCGGCCTTGTAAGCGTTGCCAGCCAACTCAATTATCGCGGGCGCAGCAACAGGTA
GAAAAAATCAGTCAGGCATGTCGGTTGATTGAACTGGCAGAAACTCCCCCTAAGCTGAATGAATTGGCAGCTCAACTGGG
GCTTAGCACTTTTTATTTTCATCGGTTGTTTAAAGCCATCACCGGGCTGACGCCCAAAGGATATGCTAACGCGACCCGCA
GTGAGCGTATTCGTGCACAACTGTCTCATGGCGGTTCGGTCACTGACGCTATCTTTGAGGCCGGTTATAACTCGAGTAGT
CGATTTTATGCGCAATCACAGCAGTTGCTGGGAATGACACCAACCCGTTACCGCAAAGGGGGCTGTGATGCCAGGTTGCA
TTTTGCCGTTGGGGAGAGTTCTCTGGGCGCGATTTTAGTGGCAAAAAGTGAGTTGGGCGTCTGTGCTATTTTGCTGGGTG
ATGACCCAGTGCGGTTAGTACAACAGCTACAGGATAAATTTCCACAGGCCAATTTAGTCGGCGGTGATGCTGAGTTTGAG
CAATGGGTGGCGCAGGTTGTGGGGTGCGTTGAGGCACCCAAACTTGGGTTGAACCTACCGTTGGATATCCGTGGCACTGC
ATTCCAGCAGCGGGTATGGCAGGCTTTGCGGGAGATACCGATAGGTGAAACCGCCAGTTATGCAGATATCGCGAGCCGAA
TCGGTTCACCCACAGCGGTACGTGCTGTCGCAGGCGCTTGTGCTGCTAACATATTGGCAGTTGCTATCCCCTGTCATCGA
GTCATTCGTCAGGATGGGGCATTATCGGGTTACCGTTGGGGCGTGGAGCGCAAGAGGCTTTTGCTGGAAAGAGAGAGTGT
GGAAAAAGAGGCGGAAGACCACTGA

Upstream 100 bases:

>100_bases
AAAAGCAAGGAGCGGAGTGTGTGACCAGTGGCATCTTTTATATGCTGGTTGGCACAGTTAAGAGTAATGGGGTATTTATC
ATGACATCAGTGGGGTATTC

Downstream 100 bases:

>100_bases
TTCTCAATATCTGCCCGAACCACATTGATCGGGCAGATAGACGGCAGATTAAGATTTAAGCGGATGCAGCGGTAGCCAGA
CAATCAGGCGTAAGCCACCT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNNVKDPRWAAIINRDKTADGQFVYAVKTTGIYCRPSCPSRRAKAEHIEFFIDNTAAEQAGYRPCKRCQPTQLSRAQQQV
EKISQACRLIELAETPPKLNELAAQLGLSTFYFHRLFKAITGLTPKGYANATRSERIRAQLSHGGSVTDAIFEAGYNSSS
RFYAQSQQLLGMTPTRYRKGGCDARLHFAVGESSLGAILVAKSELGVCAILLGDDPVRLVQQLQDKFPQANLVGGDAEFE
QWVAQVVGCVEAPKLGLNLPLDIRGTAFQQRVWQALREIPIGETASYADIASRIGSPTAVRAVAGACAANILAVAIPCHR
VIRQDGALSGYRWGVERKRLLLERESVEKEAEDH

Sequences:

>Translated_354_residues
MNNVKDPRWAAIINRDKTADGQFVYAVKTTGIYCRPSCPSRRAKAEHIEFFIDNTAAEQAGYRPCKRCQPTQLSRAQQQV
EKISQACRLIELAETPPKLNELAAQLGLSTFYFHRLFKAITGLTPKGYANATRSERIRAQLSHGGSVTDAIFEAGYNSSS
RFYAQSQQLLGMTPTRYRKGGCDARLHFAVGESSLGAILVAKSELGVCAILLGDDPVRLVQQLQDKFPQANLVGGDAEFE
QWVAQVVGCVEAPKLGLNLPLDIRGTAFQQRVWQALREIPIGETASYADIASRIGSPTAVRAVAGACAANILAVAIPCHR
VIRQDGALSGYRWGVERKRLLLERESVEKEAEDH
>Mature_354_residues
MNNVKDPRWAAIINRDKTADGQFVYAVKTTGIYCRPSCPSRRAKAEHIEFFIDNTAAEQAGYRPCKRCQPTQLSRAQQQV
EKISQACRLIELAETPPKLNELAAQLGLSTFYFHRLFKAITGLTPKGYANATRSERIRAQLSHGGSVTDAIFEAGYNSSS
RFYAQSQQLLGMTPTRYRKGGCDARLHFAVGESSLGAILVAKSELGVCAILLGDDPVRLVQQLQDKFPQANLVGGDAEFE
QWVAQVVGCVEAPKLGLNLPLDIRGTAFQQRVWQALREIPIGETASYADIASRIGSPTAVRAVAGACAANILAVAIPCHR
VIRQDGALSGYRWGVERKRLLLERESVEKEAEDH

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=160, Percent_Identity=30, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=52.463768115942, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787596, Length=89, Percent_Identity=43.8202247191011, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=129, Percent_Identity=44.1860465116279, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI115533068, Length=129, Percent_Identity=44.1860465116279, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=45.679012345679, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137554, Length=142, Percent_Identity=42.2535211267606, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38874; Mature: 38874

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVKDPRWAAIINRDKTADGQFVYAVKTTGIYCRPSCPSRRAKAEHIEFFIDNTAAEQA
CCCCCCCCEEEEECCCCCCCCCEEEEEEECCEEECCCCCCCHHHHHHHHHEECCHHHHHC
GYRPCKRCQPTQLSRAQQQVEKISQACRLIELAETPPKLNELAAQLGLSTFYFHRLFKAI
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGLTPKGYANATRSERIRAQLSHGGSVTDAIFEAGYNSSSRFYAQSQQLLGMTPTRYRKG
HCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCC
GCDARLHFAVGESSLGAILVAKSELGVCAILLGDDPVRLVQQLQDKFPQANLVGGDAEFE
CCCEEEEEEECCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCHHHH
QWVAQVVGCVEAPKLGLNLPLDIRGTAFQQRVWQALREIPIGETASYADIASRIGSPTAV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHH
RAVAGACAANILAVAIPCHRVIRQDGALSGYRWGVERKRLLLERESVEKEAEDH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNVKDPRWAAIINRDKTADGQFVYAVKTTGIYCRPSCPSRRAKAEHIEFFIDNTAAEQA
CCCCCCCCEEEEECCCCCCCCCEEEEEEECCEEECCCCCCCHHHHHHHHHEECCHHHHHC
GYRPCKRCQPTQLSRAQQQVEKISQACRLIELAETPPKLNELAAQLGLSTFYFHRLFKAI
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGLTPKGYANATRSERIRAQLSHGGSVTDAIFEAGYNSSSRFYAQSQQLLGMTPTRYRKG
HCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHCCC
GCDARLHFAVGESSLGAILVAKSELGVCAILLGDDPVRLVQQLQDKFPQANLVGGDAEFE
CCCEEEEEEECCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCHHHH
QWVAQVVGCVEAPKLGLNLPLDIRGTAFQQRVWQALREIPIGETASYADIASRIGSPTAV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHH
RAVAGACAANILAVAIPCHRVIRQDGALSGYRWGVERKRLLLERESVEKEAEDH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]