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The map label for this gene is pfkA [H]

Identifier: 170026333

GI number: 170026333

Start: 4559493

End: 4560476

Strand: Reverse

Name: pfkA [H]

Synonym: YPK_4126

Alternate gene names: 170026333

Gene position: 4560476-4559493 (Counterclockwise)

Preceding gene: 170026334

Following gene: 170026332

Centisome position: 97.25

GC content: 47.56

Gene sequence:

>984_bases
ATGGTTAAGAAAATTGGTGTCCTAACAAGTGGTGGTGATGCACCCGGTATGAATGCTGCTATTCGCGGGGTTGTTCGTGC
CGCTTTGTCAGCAGGGTTAGACGTTTTCGGTATTGAAGATGGCTATCTTGGCTTATATGAAAATCGTATGAAGAAGCTGG
ATCGCTATAGCGTGTCAGATATGATTAACCGTGGCGGTACTTTCCTCGGATCTGCTCGTTTCCCAGAATTCCGCGATCCG
GAAGTGCGTAAAGTTGCCCTCAAGAATATGCATGAGCGTGGCATTGATGGCTTGGTTGTTATCGGTGGTGACGGTTCTTA
TGCTGGTGCGGACCTGCTGACGAAAGAAGGGGGTATTCACTGTGTTGGTTTACCGGGCACTATCGATAACGATGTGGCGG
GTACTGACTACACCATCGGTTTTTTCACCGCACTGGAAACGGTAGTGGAAGCCATTGACCGCTTACGCGATACCTCTTCT
TCCCACCAACGTATCTCTATCGTGGAAGTGATGGGCCGCTATTGCGGTGATTTAACGTTGGCAGCCGCGATTGCTGGTGG
TTGTGAGTTTATTGCGATCCCTGAAGTTGAATTTAAACGTGATGATCTGGTTGCTGAAATCAAAGCGGGCATCGCGAAAG
GTAAAAAGCACGCGATTGTTGCTATTACTGAAAAGCTGGATGATATCGATTCATTGGCTAAGTACATTGAGAAAGAGACG
GGCCGTGAAACTCGTGGCACCGTGTTAGGCCATATCCAACGTGGTGGTGCTCCGGTGGCTTATGACCGCATCCTTGCTTC
CCGTATGGGGTCTTATGCTGTCGATCTGCTGTTACAGGACCACGATTACAAAAAAGGTGGTTTCTGCGTCGGTGTGCAGA
ATGAGAAGATGGTGCATGAATTAATTTCTGTGTGTATCGCGCCAGAGAATAAGAAAAGTAAATTTAAAGAAGATTGGTAC
GATACAGCGAAGAAACTGTTCTAA

Upstream 100 bases:

>100_bases
TAATCCCATAAAGCTGATTTTCTGTACGGTCTCTTCCGTGCAAAGATAATCGGCAGATAAAGAATTCTACAAAATCGCAT
TTACAAGTTCAGAGGTAGTC

Downstream 100 bases:

>100_bases
AACGGCAACTCGCTCGTTCGGCAAGCCCTCGAAATTGAACTGATCCCAAAAAGTTGGGCAGTTCACATTGCGAGGGCTTT
TTACTTTCAGCCACTCATAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSDMINRGGTFLGSARFPEFRDP
EVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIHCVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSS
SHQRISIVEVMGRYCGDLTLAAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
GRETRGTVLGHIQRGGAPVAYDRILASRMGSYAVDLLLQDHDYKKGGFCVGVQNEKMVHELISVCIAPENKKSKFKEDWY
DTAKKLF

Sequences:

>Translated_327_residues
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSDMINRGGTFLGSARFPEFRDP
EVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIHCVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSS
SHQRISIVEVMGRYCGDLTLAAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
GRETRGTVLGHIQRGGAPVAYDRILASRMGSYAVDLLLQDHDYKKGGFCVGVQNEKMVHELISVCIAPENKKSKFKEDWY
DTAKKLF
>Mature_327_residues
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSDMINRGGTFLGSARFPEFRDP
EVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIHCVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSS
SHQRISIVEVMGRYCGDLTLAAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
GRETRGTVLGHIQRGGAPVAYDRILASRMGSYAVDLLLQDHDYKKGGFCVGVQNEKMVHELISVCIAPENKKSKFKEDWY
DTAKKLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=40.8360128617363, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI266453619, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI266453768, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI266453748, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI4505749, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=41.8006430868167, Blast_Score=208, Evalue=7e-54,
Organism=Escherichia coli, GI1790350, Length=327, Percent_Identity=76.7584097859327, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=39.871382636656, Blast_Score=202, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17558788, Length=319, Percent_Identity=36.9905956112853, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=30.9278350515464, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321679, Length=311, Percent_Identity=42.443729903537, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6323861, Length=313, Percent_Identity=38.0191693290735, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28573326, Length=312, Percent_Identity=41.9871794871795, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=41.8006430868167, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=41.8006430868167, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35411; Mature: 35411

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFRDPEVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIH
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHEECCCCEE
CVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTL
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHH
AAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHC
GRETRGTVLGHIQRGGAPVAYDRILASRMGSYAVDLLLQDHDYKKGGFCVGVQNEKMVHE
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHEEEECCCCCCCCEEEECCCHHHHHH
LISVCIAPENKKSKFKEDWYDTAKKLF
HHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKKIGVLTSGGDAPGMNAAIRGVVRAALSAGLDVFGIEDGYLGLYENRMKKLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPEFRDPEVRKVALKNMHERGIDGLVVIGGDGSYAGADLLTKEGGIH
HHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHEECCCCEE
CVGLPGTIDNDVAGTDYTIGFFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTL
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHH
AAAIAGGCEFIAIPEVEFKRDDLVAEIKAGIAKGKKHAIVAITEKLDDIDSLAKYIEKET
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHC
GRETRGTVLGHIQRGGAPVAYDRILASRMGSYAVDLLLQDHDYKKGGFCVGVQNEKMVHE
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHEEEECCCCCCCCEEEECCCHHHHHH
LISVCIAPENKKSKFKEDWYDTAKKLF
HHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA