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The map label for this gene is glpK [H]

Identifier: 170026320

GI number: 170026320

Start: 4546452

End: 4547975

Strand: Direct

Name: glpK [H]

Synonym: YPK_4113

Alternate gene names: 170026320

Gene position: 4546452-4547975 (Clockwise)

Preceding gene: 170026319

Following gene: 170026321

Centisome position: 96.95

GC content: 51.97

Gene sequence:

>1524_bases
ATGACAACTGAAAATACTACTCAAAAAAAATACATTGTCGCACTTGATCAGGGGACAACCAGCTCCAGGGCGGTAGTACT
GGATCACAATGCAAATATCGTGAGTGTTTCTCAGCGTGAATTCACTCAGATTTACCCAAAGGCTGGCTGGGTTGAGCATG
ACCCAATGGAGATCTGGGCGACCCAAAGCTCTACACTGATTGAAGTCCTGGCCAAAGCCGGTATCAACTCTGATGAAATC
GCGGGTATCGGTATTACCAATCAGCGCGAAACCACGATTGTTTGGGATAAAGTGACCGGTAAGCCGGTGTATAACGCCAT
TGTCTGGCAATGCCGCCGTACTGCGGATATTTGCGAAAAACTGAAAAAAGAAGGGCTGGAAGAGTATATCCGCCACAACA
CCGGTTTGGTGGTAGATCCCTACTTCTCTGGCACAAAAGTGAAATGGATCCTCGATAACGTCGAGGGCGCTCGCGAGCGC
GCTGAACGCGGTGAGTTACTGTTTGGTACCGTAGACACCTGGCTGGTGTGGAATATGACGCAAGGCCGTGTGCACGTCAC
CGACTATACCAACGCATCACGGACCATGATGTTTAACATCCGTACCAAGGAGTGGGACGACCGTATGCTGAAAGCGCTGA
ATATTCCACGCGCCATGCTACCGGAAGTTCGCCCATCCTCCGAAATCTACGGCAAAACCAATATCGGTGGCAAAGGTGGC
ACTCGTATTCCAATCGCCGGGATTGCCGGTGACCAGCAAGCCGCTCTGTTTGGTCAGCTTTGTGTACAGCCGGGCATGGC
AAAAAATACCTACGGTACGGGTTGCTTCCTGCTGATGAACACCGGTGAAGAAGCGGTTCAATCTACTCATGGCCTGTTGA
CCACCATTGCCTGTGGCCCACGCGGTGAAGTGAATTATGCACTGGAAGGGGCTGTATTTATTGGTGGTGCCTCCATTCAA
TGGCTACGTGATGAATTGAAGTTGATAGGCGATGCCACCGATTCTGAGTATTTCGCGACCAAAGTGAAAAACAGTAATGG
TGTGTATGTGGTACCAGCGTTCACCGGTCTGGGCGCTCCTTACTGGGACCCGTATGCCCGTGGTGCGATTTTCGGCCTGA
CCCGTGGTGTTAACAGCAACCACATTATCCGTGCAACGCTCGAATCCATAGCTTATCAGACCCGCGATGTCTTGGATGCC
ATGCAAGCTGACTCCGGCGCACGGCTGAAATCACTGCGTGTTGATGGCGGTGCAGTAGCTAATAACTTCCTGATGCAGTT
CCAGGCCGATATTCTGGGTACCCGTGTTGAGCGCCCTGCTATTCGTGAAAGTACAGCATTGGGTGCTGCATTCCTGGCAG
GTCTGGCGACGGGCTTCTGGGATGACCTCGATGAGGTGAAGAGTAAAGCAAGCATTGAACGCGAATTCCGTCCAGGGATT
GAAACCACAGAGCGTGATATCCGTTACAAGGGCTGGAAAAAAGCAGTGGCCCGCGCCCGGGATTGGGAAGAGCACGACGA
GTAA

Upstream 100 bases:

>100_bases
CGTTTTGAACGCGCCTACAGAGGCCGAGTAATCAGAGTAACTATCCCCCTGCGGCTTCAAGTACGAAGGGGATAAATGGC
AACTTAAAAGTAGGCTTATT

Downstream 100 bases:

>100_bases
TAGCCCGCCAGTTTTAGCCAGTCCGTTTTAAACAGAACCGGTTTTAAACTAAGCCTATTTTAAACTGAGCCTATTTTAAA
CCAAACACAACGCGCTATCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTTENTTQKKYIVALDQGTTSSRAVVLDHNANIVSVSQREFTQIYPKAGWVEHDPMEIWATQSSTLIEVLAKAGINSDEI
AGIGITNQRETTIVWDKVTGKPVYNAIVWQCRRTADICEKLKKEGLEEYIRHNTGLVVDPYFSGTKVKWILDNVEGARER
AERGELLFGTVDTWLVWNMTQGRVHVTDYTNASRTMMFNIRTKEWDDRMLKALNIPRAMLPEVRPSSEIYGKTNIGGKGG
TRIPIAGIAGDQQAALFGQLCVQPGMAKNTYGTGCFLLMNTGEEAVQSTHGLLTTIACGPRGEVNYALEGAVFIGGASIQ
WLRDELKLIGDATDSEYFATKVKNSNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNSNHIIRATLESIAYQTRDVLDA
MQADSGARLKSLRVDGGAVANNFLMQFQADILGTRVERPAIRESTALGAAFLAGLATGFWDDLDEVKSKASIEREFRPGI
ETTERDIRYKGWKKAVARARDWEEHDE

Sequences:

>Translated_507_residues
MTTENTTQKKYIVALDQGTTSSRAVVLDHNANIVSVSQREFTQIYPKAGWVEHDPMEIWATQSSTLIEVLAKAGINSDEI
AGIGITNQRETTIVWDKVTGKPVYNAIVWQCRRTADICEKLKKEGLEEYIRHNTGLVVDPYFSGTKVKWILDNVEGARER
AERGELLFGTVDTWLVWNMTQGRVHVTDYTNASRTMMFNIRTKEWDDRMLKALNIPRAMLPEVRPSSEIYGKTNIGGKGG
TRIPIAGIAGDQQAALFGQLCVQPGMAKNTYGTGCFLLMNTGEEAVQSTHGLLTTIACGPRGEVNYALEGAVFIGGASIQ
WLRDELKLIGDATDSEYFATKVKNSNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNSNHIIRATLESIAYQTRDVLDA
MQADSGARLKSLRVDGGAVANNFLMQFQADILGTRVERPAIRESTALGAAFLAGLATGFWDDLDEVKSKASIEREFRPGI
ETTERDIRYKGWKKAVARARDWEEHDE
>Mature_506_residues
TTENTTQKKYIVALDQGTTSSRAVVLDHNANIVSVSQREFTQIYPKAGWVEHDPMEIWATQSSTLIEVLAKAGINSDEIA
GIGITNQRETTIVWDKVTGKPVYNAIVWQCRRTADICEKLKKEGLEEYIRHNTGLVVDPYFSGTKVKWILDNVEGARERA
ERGELLFGTVDTWLVWNMTQGRVHVTDYTNASRTMMFNIRTKEWDDRMLKALNIPRAMLPEVRPSSEIYGKTNIGGKGGT
RIPIAGIAGDQQAALFGQLCVQPGMAKNTYGTGCFLLMNTGEEAVQSTHGLLTTIACGPRGEVNYALEGAVFIGGASIQW
LRDELKLIGDATDSEYFATKVKNSNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNSNHIIRATLESIAYQTRDVLDAM
QADSGARLKSLRVDGGAVANNFLMQFQADILGTRVERPAIRESTALGAAFLAGLATGFWDDLDEVKSKASIEREFRPGIE
TTERDIRYKGWKKAVARARDWEEHDE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=48.00796812749, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=48.00796812749, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=47.8260869565217, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=47.0119521912351, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=34.3137254901961, Blast_Score=318, Evalue=9e-87,
Organism=Escherichia coli, GI1790361, Length=501, Percent_Identity=84.6307385229541, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=487, Percent_Identity=24.0246406570842, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790005, Length=499, Percent_Identity=24.4488977955912, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789138, Length=510, Percent_Identity=23.3333333333333, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787789, Length=504, Percent_Identity=24.6031746031746, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789168, Length=496, Percent_Identity=22.3790322580645, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=40.990099009901, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=26.1538461538462, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71986147, Length=296, Percent_Identity=31.0810810810811, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=37.0036101083032, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI17864214, Length=507, Percent_Identity=45.3648915187377, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=42.9718875502008, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=42.9718875502008, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=42.9718875502008, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=42.9718875502008, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=42.9718875502008, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24654573, Length=444, Percent_Identity=47.0720720720721, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI19922058, Length=535, Percent_Identity=33.6448598130841, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24652871, Length=497, Percent_Identity=34.20523138833, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=27.8210116731518, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=27.0321361058601, Blast_Score=205, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24656372, Length=366, Percent_Identity=30.6010928961749, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI45552909, Length=307, Percent_Identity=27.6872964169381, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 56104; Mature: 55973

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTENTTQKKYIVALDQGTTSSRAVVLDHNANIVSVSQREFTQIYPKAGWVEHDPMEIWA
CCCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCCCEEEE
TQSSTLIEVLAKAGINSDEIAGIGITNQRETTIVWDKVTGKPVYNAIVWQCRRTADICEK
CCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LKKEGLEEYIRHNTGLVVDPYFSGTKVKWILDNVEGARERAERGELLFGTVDTWLVWNMT
HHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCHHHHHHHHCCCEEEEEECEEEEEEEC
QGRVHVTDYTNASRTMMFNIRTKEWDDRMLKALNIPRAMLPEVRPSSEIYGKTNIGGKGG
CCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCC
TRIPIAGIAGDQQAALFGQLCVQPGMAKNTYGTGCFLLMNTGEEAVQSTHGLLTTIACGP
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHCCEEEEEECCC
RGEVNYALEGAVFIGGASIQWLRDELKLIGDATDSEYFATKVKNSNGVYVVPAFTGLGAP
CCCEEEEEECEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
YWDPYARGAIFGLTRGVNSNHIIRATLESIAYQTRDVLDAMQADSGARLKSLRVDGGAVA
CCCHHHCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
NNFLMQFQADILGTRVERPAIRESTALGAAFLAGLATGFWDDLDEVKSKASIEREFRPGI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
ETTERDIRYKGWKKAVARARDWEEHDE
CCCHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
TTENTTQKKYIVALDQGTTSSRAVVLDHNANIVSVSQREFTQIYPKAGWVEHDPMEIWA
CCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEEECHHHHHHHCCCCCCCCCCCCEEEE
TQSSTLIEVLAKAGINSDEIAGIGITNQRETTIVWDKVTGKPVYNAIVWQCRRTADICEK
CCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LKKEGLEEYIRHNTGLVVDPYFSGTKVKWILDNVEGARERAERGELLFGTVDTWLVWNMT
HHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCHHHHHHHHCCCEEEEEECEEEEEEEC
QGRVHVTDYTNASRTMMFNIRTKEWDDRMLKALNIPRAMLPEVRPSSEIYGKTNIGGKGG
CCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCC
TRIPIAGIAGDQQAALFGQLCVQPGMAKNTYGTGCFLLMNTGEEAVQSTHGLLTTIACGP
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHCCEEEEEECCC
RGEVNYALEGAVFIGGASIQWLRDELKLIGDATDSEYFATKVKNSNGVYVVPAFTGLGAP
CCCEEEEEECEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
YWDPYARGAIFGLTRGVNSNHIIRATLESIAYQTRDVLDAMQADSGARLKSLRVDGGAVA
CCCHHHCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHH
NNFLMQFQADILGTRVERPAIRESTALGAAFLAGLATGFWDDLDEVKSKASIEREFRPGI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
ETTERDIRYKGWKKAVARARDWEEHDE
CCCHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]