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The map label for this gene is glpF [H]

Identifier: 170026319

GI number: 170026319

Start: 4545427

End: 4546275

Strand: Direct

Name: glpF [H]

Synonym: YPK_4112

Alternate gene names: 170026319

Gene position: 4545427-4546275 (Clockwise)

Preceding gene: 170026317

Following gene: 170026320

Centisome position: 96.93

GC content: 50.88

Gene sequence:

>849_bases
ATGAGCCAAACCGCTAGTTCTACCTTAAAAGGCCAATGTATTGCGGAGTTTCTCGGTACTGGGCTATTGATATTTTTTGG
TGCAGGTTGTGTTGCTGCTTTGAAGTTGGCAGGGGCCAGTTTTGGGCAGTGGGAAATCAGTATCATTTGGGGCCTGGGTG
TTGCTATGGCCATTTATTTGACGGCAGCCATTTCAGGCGCGCATCTTAATCCGGCCGTCACCATCGCATTGTGGTTATTC
GCCTGTTTCGAACGCCGTAAAGTTCTCCCTTACATCGTGGCACAGGTTGCCGGCGCATTTTGTGCCGCGGCGTTAGTATA
CGGCCTGTATTACAGCCTGTTCGTCGATTTTGAACAAACTCACCAGATGGTGCGTGGCAGCACCGAGAGCCTCCAACTGG
CAGGTATCTTCTCTACTTACCCAAATCCACATATTTCTGTCGTTCAAGCTTTCTTGGTTGAAACAGTCATTACCGCCATT
CTGATGTGTCTGATTCTGGCACTGACTGATGATGGTAACGGCATTCCACGCGGCCCGCTGGCCCCCCTACTGATCGGTAT
TCTGATCGCAGTTATTGGTGCTTCAATGGGGCCACTGACCGGGTTCGCACTCAACCCAGCACGTGACCTTGGTCCAAAAG
CATTCTCTTATCTGGCAGGCTGGGGTGATATTGCCTTCACTGGTGGGCGCGATATTCCTTATTTCCTGGTACCGATCTTC
GGGCCTATCGTCGGTGCTTTGTTGGGTGCATTTGGTTATCGGGCCTTAATTGGCCGCCACCTGCCCTGTGATGTGTGTAC
CCTTGAGGATGAAGAATCCACAACGATAACCACAGAACGTAAAGCATAA

Upstream 100 bases:

>100_bases
TCATTTTCGCTCGTTAACGATAAATTTACATTATCCCCTTCACTGCTCGAAGGGCAAAAACAAGAAATACCCTATAAATC
TCTGTCTGTAGGACATTATT

Downstream 100 bases:

>100_bases
ATAATAGATACTGAAATATACACAAGTCATTGGAGTCGCATGTAGGCAGCAAACGCACAACAAATGGGTTATAAACCGTT
TTGAACGCGCCTACAGAGGC

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSQTASSTLKGQCIAEFLGTGLLIFFGAGCVAALKLAGASFGQWEISIIWGLGVAMAIYLTAAISGAHLNPAVTIALWLF
ACFERRKVLPYIVAQVAGAFCAAALVYGLYYSLFVDFEQTHQMVRGSTESLQLAGIFSTYPNPHISVVQAFLVETVITAI
LMCLILALTDDGNGIPRGPLAPLLIGILIAVIGASMGPLTGFALNPARDLGPKAFSYLAGWGDIAFTGGRDIPYFLVPIF
GPIVGALLGAFGYRALIGRHLPCDVCTLEDEESTTITTERKA

Sequences:

>Translated_282_residues
MSQTASSTLKGQCIAEFLGTGLLIFFGAGCVAALKLAGASFGQWEISIIWGLGVAMAIYLTAAISGAHLNPAVTIALWLF
ACFERRKVLPYIVAQVAGAFCAAALVYGLYYSLFVDFEQTHQMVRGSTESLQLAGIFSTYPNPHISVVQAFLVETVITAI
LMCLILALTDDGNGIPRGPLAPLLIGILIAVIGASMGPLTGFALNPARDLGPKAFSYLAGWGDIAFTGGRDIPYFLVPIF
GPIVGALLGAFGYRALIGRHLPCDVCTLEDEESTTITTERKA
>Mature_281_residues
SQTASSTLKGQCIAEFLGTGLLIFFGAGCVAALKLAGASFGQWEISIIWGLGVAMAIYLTAAISGAHLNPAVTIALWLFA
CFERRKVLPYIVAQVAGAFCAAALVYGLYYSLFVDFEQTHQMVRGSTESLQLAGIFSTYPNPHISVVQAFLVETVITAIL
MCLILALTDDGNGIPRGPLAPLLIGILIAVIGASMGPLTGFALNPARDLGPKAFSYLAGWGDIAFTGGRDIPYFLVPIFG
PIVGALLGAFGYRALIGRHLPCDVCTLEDEESTTITTERKA

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=270, Percent_Identity=40.7407407407407, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI4826645, Length=272, Percent_Identity=38.9705882352941, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI22538420, Length=258, Percent_Identity=39.1472868217054, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4502187, Length=260, Percent_Identity=36.5384615384615, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310133356, Length=206, Percent_Identity=33.495145631068, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI310114181, Length=206, Percent_Identity=33.495145631068, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI86792455, Length=245, Percent_Identity=33.469387755102, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4502179, Length=286, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45446752, Length=206, Percent_Identity=29.6116504854369, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4502183, Length=240, Percent_Identity=30.4166666666667, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI37694062, Length=209, Percent_Identity=29.6650717703349, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI6912506, Length=258, Percent_Identity=29.0697674418605, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790362, Length=282, Percent_Identity=82.6241134751773, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1787101, Length=213, Percent_Identity=32.3943661971831, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994009, Length=271, Percent_Identity=35.4243542435424, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17533613, Length=266, Percent_Identity=35.7142857142857, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32564052, Length=266, Percent_Identity=35.7142857142857, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71992966, Length=270, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17544068, Length=268, Percent_Identity=36.9402985074627, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531431, Length=276, Percent_Identity=33.695652173913, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531429, Length=270, Percent_Identity=33.7037037037037, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71992961, Length=270, Percent_Identity=30, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71993722, Length=246, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=37.962962962963, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=311, Percent_Identity=29.2604501607717, Blast_Score=105, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45550503, Length=270, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762344, Length=200, Percent_Identity=32, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24762346, Length=200, Percent_Identity=32, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24762348, Length=200, Percent_Identity=32, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20130305, Length=200, Percent_Identity=32, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24762342, Length=245, Percent_Identity=27.7551020408163, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652747, Length=251, Percent_Identity=27.0916334661355, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551084, Length=251, Percent_Identity=27.0916334661355, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29785; Mature: 29654

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTASSTLKGQCIAEFLGTGLLIFFGAGCVAALKLAGASFGQWEISIIWGLGVAMAIYL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
TAAISGAHLNPAVTIALWLFACFERRKVLPYIVAQVAGAFCAAALVYGLYYSLFVDFEQT
HHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQMVRGSTESLQLAGIFSTYPNPHISVVQAFLVETVITAILMCLILALTDDGNGIPRGPL
HHHHCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
APLLIGILIAVIGASMGPLTGFALNPARDLGPKAFSYLAGWGDIAFTGGRDIPYFLVPIF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCEEEECCCCCCCHHHHHH
GPIVGALLGAFGYRALIGRHLPCDVCTLEDEESTTITTERKA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEECCCCC
>Mature Secondary Structure 
SQTASSTLKGQCIAEFLGTGLLIFFGAGCVAALKLAGASFGQWEISIIWGLGVAMAIYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
TAAISGAHLNPAVTIALWLFACFERRKVLPYIVAQVAGAFCAAALVYGLYYSLFVDFEQT
HHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQMVRGSTESLQLAGIFSTYPNPHISVVQAFLVETVITAILMCLILALTDDGNGIPRGPL
HHHHCCCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
APLLIGILIAVIGASMGPLTGFALNPARDLGPKAFSYLAGWGDIAFTGGRDIPYFLVPIF
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCEEEECCCCCCCHHHHHH
GPIVGALLGAFGYRALIGRHLPCDVCTLEDEESTTITTERKA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]