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The map label for this gene is trmA [H]

Identifier: 170026281

GI number: 170026281

Start: 4499085

End: 4500188

Strand: Direct

Name: trmA [H]

Synonym: YPK_4074

Alternate gene names: 170026281

Gene position: 4499085-4500188 (Clockwise)

Preceding gene: 170026274

Following gene: 170026285

Centisome position: 95.94

GC content: 47.83

Gene sequence:

>1104_bases
ATGACTCCCAACATCCTGCCGATTGAAAGCTACGACCACCAACTGGCAGAAAAGTCTGCCCGTCTTAAGGCGATGATGTT
GCCATTTCAGGCACCCGAGCCAGAAATATTTCGTTCTCCGGCAGACCATTACCGTATGAGAGCCGAGTTTCGCGTTTGGC
ATGATGAAGATGACTTGTACCACATCATGTTTGACCAACAGACCAAGCAACGTATCCGGGTTGAACAATTCCCGGTAGCC
AGTCGGTTAATCAATCGCTTAATGGATGCTCTGATGACCGCCATCAGAGCGGAGCCGCTATTGCGACGCAAACTGTTCCA
AATAGATTATTTATCTACCCTCAGCGGTAAGCTGATCGCCTCGTTGCTGTATCACCGCCAATTAGATGAAGAGTGGCAGC
AAAAAGCGCTGGAACTGCGAGATCAGTTACGTGCACAAGGTTTTGACCTACAACTCATTGGTCGAGCAGCTAAAACTAAA
ATCATGCTGGATCACGATTATATCGATGAGGTCTTGCCAGTCGCCGGACGCGAGATGATCTATCGGCAGGTTGAAAATAG
TTTTACCCAACCTAATGCGGCGGTGAATATTCACATGTTGGAATGGGCGCTGGATGTTACCCAAGGTGCCACTGGCGATT
TACTGGAGTTGTATTGTGGTAACGGTAATTTCTCATTGGCACTGGCGCGAAATTTTGAACGGGTGCTGGCAACAGAAATT
GCCAAACCTTCCGTCGCAGCGGCTCAATATAATATTGCAGCAAATAATATCGATAACGTGCAGATTATCCGGATGTCAGC
GGAAGAGTTTACTCAGGCGATGCAGGGCGTTCGTGAGTTTAACCGCCTGAAGGGTATTGATTTGGGCAGTTATAATTGCG
AGACCATCTTTGTCGATCCGCCACGCAGTGGGCTGGATCACGAGACGGTTAAGCTAGTACAGGCTTACCCGCGTATTCTT
TATATCTCCTGTAATCCAGAGACGCTGTGCGCCAATTTGGAACAATTACAGCACACACATAAAATTAGCCGACTAGCACT
GTTCGATCAATTCCCTTATACCCACCATATGGAATGTGGGGTACTGCTGGAAAAACGTCACTGA

Upstream 100 bases:

>100_bases
ATCCACGCCGGAATAAACACGACAAAACTGGACATCAGGGCAACTTTCCCTACAATCCGCAAGGATCATCCCCCTTTTAT
ACTGACCCAACGAGAAAAAC

Downstream 100 bases:

>100_bases
CGAATAATTCATACCGCAGATAAAAGAAAGGGCAACGACATGTTGCCCTTAGTTGGCAAGACGGTATCTAGGTGCAGTAA
CCCCTTCGAGCACCGCGCTA

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTPNILPIESYDHQLAEKSARLKAMMLPFQAPEPEIFRSPADHYRMRAEFRVWHDEDDLYHIMFDQQTKQRIRVEQFPVA
SRLINRLMDALMTAIRAEPLLRRKLFQIDYLSTLSGKLIASLLYHRQLDEEWQQKALELRDQLRAQGFDLQLIGRAAKTK
IMLDHDYIDEVLPVAGREMIYRQVENSFTQPNAAVNIHMLEWALDVTQGATGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANNIDNVQIIRMSAEEFTQAMQGVREFNRLKGIDLGSYNCETIFVDPPRSGLDHETVKLVQAYPRIL
YISCNPETLCANLEQLQHTHKISRLALFDQFPYTHHMECGVLLEKRH

Sequences:

>Translated_367_residues
MTPNILPIESYDHQLAEKSARLKAMMLPFQAPEPEIFRSPADHYRMRAEFRVWHDEDDLYHIMFDQQTKQRIRVEQFPVA
SRLINRLMDALMTAIRAEPLLRRKLFQIDYLSTLSGKLIASLLYHRQLDEEWQQKALELRDQLRAQGFDLQLIGRAAKTK
IMLDHDYIDEVLPVAGREMIYRQVENSFTQPNAAVNIHMLEWALDVTQGATGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANNIDNVQIIRMSAEEFTQAMQGVREFNRLKGIDLGSYNCETIFVDPPRSGLDHETVKLVQAYPRIL
YISCNPETLCANLEQLQHTHKISRLALFDQFPYTHHMECGVLLEKRH
>Mature_366_residues
TPNILPIESYDHQLAEKSARLKAMMLPFQAPEPEIFRSPADHYRMRAEFRVWHDEDDLYHIMFDQQTKQRIRVEQFPVAS
RLINRLMDALMTAIRAEPLLRRKLFQIDYLSTLSGKLIASLLYHRQLDEEWQQKALELRDQLRAQGFDLQLIGRAAKTKI
MLDHDYIDEVLPVAGREMIYRQVENSFTQPNAAVNIHMLEWALDVTQGATGDLLELYCGNGNFSLALARNFERVLATEIA
KPSVAAAQYNIAANNIDNVQIIRMSAEEFTQAMQGVREFNRLKGIDLGSYNCETIFVDPPRSGLDHETVKLVQAYPRILY
ISCNPETLCANLEQLQHTHKISRLALFDQFPYTHHMECGVLLEKRH

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=366, Percent_Identity=76.775956284153, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1789148, Length=185, Percent_Identity=34.0540540540541, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787083, Length=184, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666579, Length=169, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 42413; Mature: 42282

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNILPIESYDHQLAEKSARLKAMMLPFQAPEPEIFRSPADHYRMRAEFRVWHDEDDLY
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCHHHHHEEEEEEEEECCCCEE
HIMFDQQTKQRIRVEQFPVASRLINRLMDALMTAIRAEPLLRRKLFQIDYLSTLSGKLIA
EEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDEEWQQKALELRDQLRAQGFDLQLIGRAAKTKIMLDHDYIDEVLPVAGREMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHEEEEECHHHHHHHHHHHHHHHH
YRQVENSFTQPNAAVNIHMLEWALDVTQGATGDLLELYCGNGNFSLALARNFERVLATEI
HHHHHHHCCCCCCEEEEEEEEEEHHHCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHH
AKPSVAAAQYNIAANNIDNVQIIRMSAEEFTQAMQGVREFNRLKGIDLGSYNCETIFVDP
CCCCHHHHEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PRSGLDHETVKLVQAYPRILYISCNPETLCANLEQLQHTHKISRLALFDQFPYTHHMECG
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLEKRH
EEEECCC
>Mature Secondary Structure 
TPNILPIESYDHQLAEKSARLKAMMLPFQAPEPEIFRSPADHYRMRAEFRVWHDEDDLY
CCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHHHCCCHHHHHEEEEEEEEECCCCEE
HIMFDQQTKQRIRVEQFPVASRLINRLMDALMTAIRAEPLLRRKLFQIDYLSTLSGKLIA
EEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDEEWQQKALELRDQLRAQGFDLQLIGRAAKTKIMLDHDYIDEVLPVAGREMI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHEEEEECHHHHHHHHHHHHHHHH
YRQVENSFTQPNAAVNIHMLEWALDVTQGATGDLLELYCGNGNFSLALARNFERVLATEI
HHHHHHHCCCCCCEEEEEEEEEEHHHCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHH
AKPSVAAAQYNIAANNIDNVQIIRMSAEEFTQAMQGVREFNRLKGIDLGSYNCETIFVDP
CCCCHHHHEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PRSGLDHETVKLVQAYPRILYISCNPETLCANLEQLQHTHKISRLALFDQFPYTHHMECG
CCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLEKRH
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA