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The map label for this gene is btuB [H]

Identifier: 170026280

GI number: 170026280

Start: 4496731

End: 4498623

Strand: Reverse

Name: btuB [H]

Synonym: YPK_4073

Alternate gene names: 170026280

Gene position: 4498623-4496731 (Counterclockwise)

Preceding gene: 170026282

Following gene: 170026279

Centisome position: 95.93

GC content: 45.22

Gene sequence:

>1893_bases
ATGATAATTAAAAATACAATGACAATTAAAAAATATACGCTGCTCACAGCCCTTTCTGTGACGGCATTTTCTGGCTGGGC
GCAGGGTAATAACACCACGGACAACAATAATGAAATGGTTGTAACAGCCAACCGTTTTCCACAACCGAAAAGTAGTGTAT
TAGCGCCTGTCGATGTAGTCACTCGTGCCGATATTGATCGCTGGCAATCTACAAATATAAATGATGTCTTACGCCGCTTA
CCGGGTATCAATATTGCCCAATATGGTGGGCCAAGACAGCTGAGCTCGCTATTTATCCGGGGGACCAATTCTAGCCATGT
CTTGGTTCTGGTCGATGGTGTTCGCCTTAATCAGGCCGGTATCAGTGGTTCTTCAGACCTAAGCCAAATCCCTATCTCAT
TAGTGCAACGTATCGAGTATATCCGCGGGCCACGTTCAGCTGTGTATGGTTCTGATGCTATTGGGGGGGTAGTAAACATT
ATCACTGAGCGTGAAACGTTGGGAAGTACCCTCACGGCAGGGTTAGGTTCGAACGGTTATCAAAATTACAATGGCTCAAC
GCAGCAAAAACTGGGTGACGACACAACAATCACTTTGGCTGGCAATTACGATTACAGCAAAGGTTATGATGTGGTTGCCA
AGGGAAATACTGGCATGGCCAGTCAGCCAGATCGTGACGGTTATCTCGGGAAAATGTTGTGGCTGGGCGCTAACCACAAA
TTTAATGAGCAATTCAGCGGTTTTGTCCGTGGCTATGGTTTTGATAATCGTAGTGACTACGACAGTTATTATTATCCGGG
TAGCCCTCTGGTAGATACACGATCACTATCTAGCCGAACATATGATACTGGAATAAATTTCAGTAATGGCGGTTATGCTT
CTCAACTGATTGGCAGTTACAGTCGAACTCAAGACTATAATTATGATCCTAGCTATGGTCGCTATGATCAATCAGCCACT
TTGGATGATATTTCCCAATACAATCTTCAGTGGACCAATACCTATCAACTTGGCTTAGGTAATGTAGGCGGCGGCCTTGA
TTGGCAGAAACAAACTACTGAGCCAGGTACTAATTACTTATCCAATGGTTATGAACAGCGAAATACCGGTGTTTACGGTA
CCGTACAGCAATTTGTCGGGCCAGTAACATTGGAAGGTGCTATTCGTGGTGATGACAACTCGCAGTTTGGCTGGCACACC
ACTTGGCAAAGTAGTGCTGGTTGGGAGTTTGTTGATGGCTATCGTTTAATCGGTTCTTATGGTACTGCATTTAAGGCACC
AAATTTGGGGCAGATATACAGTTCAACTTACGGTAATCGGGATCTTAAGCCAGAGGAAAGTACCCAATGGGAAGCCGCAA
TTACGGGGATCACGGGACCACTTGATTGGCGTTTATCGGCTTATCGTAATGATATTGATCAGATGATTGCGACCAGAGGG
GTATATCCAAACTCCCGCTATTATAATGTGGAGAAAGCCACGATTAAAGGTGTTGAATGGACAGGTTCGTTTGAGACTGG
GCCGTTATCACATCAAGTGACGCTGGAATATCTGGACCCACGTAATGCAGATACCCACGAGATTCTGGCGCGCCGGGCCA
AACAACAAGTTAAATATCAGTTGGATTGGCAGATGGCTGATTTGGATTGGTCCGTTACCTACCAATATATTGGCCAGCGT
TATGACAGTGTTTTCGATCCAATAACTTATGCGGCAAGCCCGGTAAAGTTAGCTGGAATTAGCCTCTGGGATCTCGCTGT
TTCGTATCCGGTCACATCTCATCTCACGGTTCGTGGTAGAATTGCCAACCTGTTTGATAAAGATTATGAGATGGTTTATG
GCTACCAAACCCCAGGACGCGAATACTACTTCACGGGAAGCTATAACTTCTAA

Upstream 100 bases:

>100_bases
CCCGAAGACCTGCCGGTATTACGTCGCAATATTCGTGGCCGTCGCGTACTACCGGCCATGGCACTGCGGCATCCGCCAAT
TGAGTTTGGATGCTCTCTTA

Downstream 100 bases:

>100_bases
GGCTGACTCTCCTCCTCGCCCGACAGCACTGATTTTTGATTCGGGTGTTGGCGGGCTGTCTGTCTATCAAGAGATTCGGC
AACTGTTGCCGAATCTCCAC

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNNEMVVTANRFPQPKSSVLAPVDVVTRADIDRWQSTNINDVLRRL
PGINIAQYGGPRQLSSLFIRGTNSSHVLVLVDGVRLNQAGISGSSDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVVNI
ITERETLGSTLTAGLGSNGYQNYNGSTQQKLGDDTTITLAGNYDYSKGYDVVAKGNTGMASQPDRDGYLGKMLWLGANHK
FNEQFSGFVRGYGFDNRSDYDSYYYPGSPLVDTRSLSSRTYDTGINFSNGGYASQLIGSYSRTQDYNYDPSYGRYDQSAT
LDDISQYNLQWTNTYQLGLGNVGGGLDWQKQTTEPGTNYLSNGYEQRNTGVYGTVQQFVGPVTLEGAIRGDDNSQFGWHT
TWQSSAGWEFVDGYRLIGSYGTAFKAPNLGQIYSSTYGNRDLKPEESTQWEAAITGITGPLDWRLSAYRNDIDQMIATRG
VYPNSRYYNVEKATIKGVEWTGSFETGPLSHQVTLEYLDPRNADTHEILARRAKQQVKYQLDWQMADLDWSVTYQYIGQR
YDSVFDPITYAASPVKLAGISLWDLAVSYPVTSHLTVRGRIANLFDKDYEMVYGYQTPGREYYFTGSYNF

Sequences:

>Translated_630_residues
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNNEMVVTANRFPQPKSSVLAPVDVVTRADIDRWQSTNINDVLRRL
PGINIAQYGGPRQLSSLFIRGTNSSHVLVLVDGVRLNQAGISGSSDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVVNI
ITERETLGSTLTAGLGSNGYQNYNGSTQQKLGDDTTITLAGNYDYSKGYDVVAKGNTGMASQPDRDGYLGKMLWLGANHK
FNEQFSGFVRGYGFDNRSDYDSYYYPGSPLVDTRSLSSRTYDTGINFSNGGYASQLIGSYSRTQDYNYDPSYGRYDQSAT
LDDISQYNLQWTNTYQLGLGNVGGGLDWQKQTTEPGTNYLSNGYEQRNTGVYGTVQQFVGPVTLEGAIRGDDNSQFGWHT
TWQSSAGWEFVDGYRLIGSYGTAFKAPNLGQIYSSTYGNRDLKPEESTQWEAAITGITGPLDWRLSAYRNDIDQMIATRG
VYPNSRYYNVEKATIKGVEWTGSFETGPLSHQVTLEYLDPRNADTHEILARRAKQQVKYQLDWQMADLDWSVTYQYIGQR
YDSVFDPITYAASPVKLAGISLWDLAVSYPVTSHLTVRGRIANLFDKDYEMVYGYQTPGREYYFTGSYNF
>Mature_630_residues
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNNEMVVTANRFPQPKSSVLAPVDVVTRADIDRWQSTNINDVLRRL
PGINIAQYGGPRQLSSLFIRGTNSSHVLVLVDGVRLNQAGISGSSDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVVNI
ITERETLGSTLTAGLGSNGYQNYNGSTQQKLGDDTTITLAGNYDYSKGYDVVAKGNTGMASQPDRDGYLGKMLWLGANHK
FNEQFSGFVRGYGFDNRSDYDSYYYPGSPLVDTRSLSSRTYDTGINFSNGGYASQLIGSYSRTQDYNYDPSYGRYDQSAT
LDDISQYNLQWTNTYQLGLGNVGGGLDWQKQTTEPGTNYLSNGYEQRNTGVYGTVQQFVGPVTLEGAIRGDDNSQFGWHT
TWQSSAGWEFVDGYRLIGSYGTAFKAPNLGQIYSSTYGNRDLKPEESTQWEAAITGITGPLDWRLSAYRNDIDQMIATRG
VYPNSRYYNVEKATIKGVEWTGSFETGPLSHQVTLEYLDPRNADTHEILARRAKQQVKYQLDWQMADLDWSVTYQYIGQR
YDSVFDPITYAASPVKLAGISLWDLAVSYPVTSHLTVRGRIANLFDKDYEMVYGYQTPGREYYFTGSYNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=622, Percent_Identity=63.6655948553055, Blast_Score=797, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=666, Percent_Identity=27.9279279279279, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786798, Length=272, Percent_Identity=28.6764705882353, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70194; Mature: 70194

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNNEMVVTANRFPQPKSSVLAPVDVV
CEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHH
TRADIDRWQSTNINDVLRRLPGINIAQYGGPRQLSSLFIRGTNSSHVLVLVDGVRLNQAG
HHCCHHHHCCCCHHHHHHHCCCCCEECCCCHHHHEEEEEECCCCCEEEEEEECEEEECCC
ISGSSDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVVNIITERETLGSTLTAGLGSNGY
CCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHCHHHHCCHHHHCCCCCCC
QNYNGSTQQKLGDDTTITLAGNYDYSKGYDVVAKGNTGMASQPDRDGYLGKMLWLGANHK
CCCCCCHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCC
FNEQFSGFVRGYGFDNRSDYDSYYYPGSPLVDTRSLSSRTYDTGINFSNGGYASQLIGSY
CCHHHHHHHEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRTQDYNYDPSYGRYDQSATLDDISQYNLQWTNTYQLGLGNVGGGLDWQKQTTEPGTNYL
HHCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHH
SNGYEQRNTGVYGTVQQFVGPVTLEGAIRGDDNSQFGWHTTWQSSAGWEFVDGYRLIGSY
HCCCHHCCCCCEEEHHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCEEECCEEEEECC
GTAFKAPNLGQIYSSTYGNRDLKPEESTQWEAAITGITGPLDWRLSAYRNDIDQMIATRG
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
VYPNSRYYNVEKATIKGVEWTGSFETGPLSHQVTLEYLDPRNADTHEILARRAKQQVKYQ
CCCCCCEEEEEEEEEECEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCEEE
LDWQMADLDWSVTYQYIGQRYDSVFDPITYAASPVKLAGISLWDLAVSYPVTSHLTVRGR
EEEEEECCCHHHHHHHHHHHHHHHHCCHHHCCCCEEEECEEEEHHEECCCCCCEEEEEEH
IANLFDKDYEMVYGYQTPGREYYFTGSYNF
HHHHHCCCCEEEEEECCCCCEEEEECCCCC
>Mature Secondary Structure
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNNEMVVTANRFPQPKSSVLAPVDVV
CEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHH
TRADIDRWQSTNINDVLRRLPGINIAQYGGPRQLSSLFIRGTNSSHVLVLVDGVRLNQAG
HHCCHHHHCCCCHHHHHHHCCCCCEECCCCHHHHEEEEEECCCCCEEEEEEECEEEECCC
ISGSSDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVVNIITERETLGSTLTAGLGSNGY
CCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHCHHHHCCHHHHCCCCCCC
QNYNGSTQQKLGDDTTITLAGNYDYSKGYDVVAKGNTGMASQPDRDGYLGKMLWLGANHK
CCCCCCHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCC
FNEQFSGFVRGYGFDNRSDYDSYYYPGSPLVDTRSLSSRTYDTGINFSNGGYASQLIGSY
CCHHHHHHHEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRTQDYNYDPSYGRYDQSATLDDISQYNLQWTNTYQLGLGNVGGGLDWQKQTTEPGTNYL
HHCCCCCCCCCCCCCCCCCCHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHH
SNGYEQRNTGVYGTVQQFVGPVTLEGAIRGDDNSQFGWHTTWQSSAGWEFVDGYRLIGSY
HCCCHHCCCCCEEEHHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCEEECCEEEEECC
GTAFKAPNLGQIYSSTYGNRDLKPEESTQWEAAITGITGPLDWRLSAYRNDIDQMIATRG
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
VYPNSRYYNVEKATIKGVEWTGSFETGPLSHQVTLEYLDPRNADTHEILARRAKQQVKYQ
CCCCCCEEEEEEEEEECEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCEEE
LDWQMADLDWSVTYQYIGQRYDSVFDPITYAASPVKLAGISLWDLAVSYPVTSHLTVRGR
EEEEEECCCHHHHHHHHHHHHHHHHCCHHHCCCCEEEECEEEEHHEECCCCCCEEEEEEH
IANLFDKDYEMVYGYQTPGREYYFTGSYNF
HHHHHCCCCEEEEEECCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA