Click here to switch to the map view.

The map label for this gene is ilvA [H]

Identifier: 170026266

GI number: 170026266

Start: 4474204

End: 4475748

Strand: Reverse

Name: ilvA [H]

Synonym: YPK_4056

Alternate gene names: 170026266

Gene position: 4475748-4474204 (Counterclockwise)

Preceding gene: 170026267

Following gene: 170026263

Centisome position: 95.44

GC content: 53.59

Gene sequence:

>1545_bases
ATGGCAGTTTCGCAACCCTTATCTGCTGCCCCTTGCGGGGCAGAATATCTGCGGGCGATATTACGTGCGCCGGTGTATGA
GGTGGCGCAAGTCACCCCGTTACAAGTGATGGAGAAAATCTCCTCGCGCGTGGGTAATACCGTTTTAGTGAAGCGTGAAG
ATCGGCAGCCGGTACACAGCTTTAAGTTGCGCGGTGCTTACGCGATGATCTCCAGCCTGACGGAAGAGCAGAAAGCCTGT
GGCGTCGTCACGGCTTCTGCGGGTAACCATGCTCAAGGCGTGGCGCTTTCTGCCCATAAAATGGGGATTAAAGCGCTGAT
CGTCATGCCCGTTGCTACTGCAGATATTAAAGTTGATGCGGTACGTGCTTTTGGCGGCGAGGTGCTGCTGTTTGGCGCTA
ATTTTGATGAAGCCAAAACCAAAGCTATCGCGCTTGCTCAAGAGCAGGGCTACACCTTTGTGCCACCGTTTGACCATCCG
GCGGTGATTGCCGGGCAGGGGACGCTGGCGATGGAGTTACTGCAACAAGATGCCCATCTTGATCGGGTTTTTGTCCCAGT
GGGCGGCGGTGGGCTGGTGGCCGGTGTGGCGGTGCTTATCAAGCAATTGATGCCACAGATTAAAGTGATTGGTGTTGAAG
CCGAGGATTCTGCCTGTTTGCGGGCCGCGCTGGATGCGGGGCAGCCGGTCGATTTGGCGCGTGTCGGGCTGTTTGCCGAG
GGCGTGGCAGTTAAGCGTATCGGTGACGAGCCTTTCCGCCTGTGTCAGGAATATCTGGACGATGTGATCACCGTTGACAG
TGATGCTATCTGTGCCGCAGTCAAAGATCTGTTTGAGGATGTTCGCGCCATTGCTGAGCCTTCTGGTGCGTTGGCATTGG
CGGGCCTGAAAAAATATGTGCAGCAGCATAATATTCAGGGCGAGCGTCTGGCACATGTGCTTTCTGGTGCAAACGTGAAC
TTCCATGGTTTGCGTTATGTGTCAGAGCGTTGCGAGTTAGGTGAGCAGCGTGAAGCCCTGCTGGCGGTGACTATTCCAGA
GCAAAAGGGCAGTTTCCTGCGTTTCTGTGAATTACTGGGTGGCCGTTCGGTAACGGAATTTAACTACCGTTACGCCGATG
CTGAAAACGCCTGCATTTTTGTTGGGGTACGTTTGACTCGTGGTTATGCTGAGCGGGTTGAAATTCTTGCTGAATTGCAG
GACAAAGGTTATCAGGTGGTCGATTTATCTGATGACGAAATGGCGAAACTGCATGTGCGTTATATGGTTGGTGGGCGGCC
CTCGAAACCACTGCGTGAGCGCCTGTTCAGCTTTGAATTCCCCGAGTCACCAGGCGCTTTGCTGAAATTCCTGCATACGT
TGGGCACTCACTGGAATATTTCTCTGTTCCACTACCGCAGCCATGGCACAGATTTTGGCCGGGTATTGGCGGCATTTGAG
TTGTCGGCTACCGAACCGCAATTTGAAGAGCGCTTAGCGGCTCTGGGCTACTATTGCCACGATGAAACGGATAACCCGGC
GTTTAAGTTCTTTTTAGCGGGTTAA

Upstream 100 bases:

>100_bases
AGCGCCAGGTCTCTTATGCCTTACGCGCTTACGCGATGTTAGCAACCAGCGCTGATAAAGGCGCGGTGCGCGATAAAAGT
AAGCTGGGAGGCTAACACCC

Downstream 100 bases:

>100_bases
TCGCGATGCATACCCGTCGTACTTTAAGCTGCAGAGGTGAACGAAAGCAATTAGCACATATGCAGCTTGGCGTCTGACGG
GTATATCACTTCTGCTGTTC

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MAVSQPLSAAPCGAEYLRAILRAPVYEVAQVTPLQVMEKISSRVGNTVLVKREDRQPVHSFKLRGAYAMISSLTEEQKAC
GVVTASAGNHAQGVALSAHKMGIKALIVMPVATADIKVDAVRAFGGEVLLFGANFDEAKTKAIALAQEQGYTFVPPFDHP
AVIAGQGTLAMELLQQDAHLDRVFVPVGGGGLVAGVAVLIKQLMPQIKVIGVEAEDSACLRAALDAGQPVDLARVGLFAE
GVAVKRIGDEPFRLCQEYLDDVITVDSDAICAAVKDLFEDVRAIAEPSGALALAGLKKYVQQHNIQGERLAHVLSGANVN
FHGLRYVSERCELGEQREALLAVTIPEQKGSFLRFCELLGGRSVTEFNYRYADAENACIFVGVRLTRGYAERVEILAELQ
DKGYQVVDLSDDEMAKLHVRYMVGGRPSKPLRERLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFE
LSATEPQFEERLAALGYYCHDETDNPAFKFFLAG

Sequences:

>Translated_514_residues
MAVSQPLSAAPCGAEYLRAILRAPVYEVAQVTPLQVMEKISSRVGNTVLVKREDRQPVHSFKLRGAYAMISSLTEEQKAC
GVVTASAGNHAQGVALSAHKMGIKALIVMPVATADIKVDAVRAFGGEVLLFGANFDEAKTKAIALAQEQGYTFVPPFDHP
AVIAGQGTLAMELLQQDAHLDRVFVPVGGGGLVAGVAVLIKQLMPQIKVIGVEAEDSACLRAALDAGQPVDLARVGLFAE
GVAVKRIGDEPFRLCQEYLDDVITVDSDAICAAVKDLFEDVRAIAEPSGALALAGLKKYVQQHNIQGERLAHVLSGANVN
FHGLRYVSERCELGEQREALLAVTIPEQKGSFLRFCELLGGRSVTEFNYRYADAENACIFVGVRLTRGYAERVEILAELQ
DKGYQVVDLSDDEMAKLHVRYMVGGRPSKPLRERLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFE
LSATEPQFEERLAALGYYCHDETDNPAFKFFLAG
>Mature_513_residues
AVSQPLSAAPCGAEYLRAILRAPVYEVAQVTPLQVMEKISSRVGNTVLVKREDRQPVHSFKLRGAYAMISSLTEEQKACG
VVTASAGNHAQGVALSAHKMGIKALIVMPVATADIKVDAVRAFGGEVLLFGANFDEAKTKAIALAQEQGYTFVPPFDHPA
VIAGQGTLAMELLQQDAHLDRVFVPVGGGGLVAGVAVLIKQLMPQIKVIGVEAEDSACLRAALDAGQPVDLARVGLFAEG
VAVKRIGDEPFRLCQEYLDDVITVDSDAICAAVKDLFEDVRAIAEPSGALALAGLKKYVQQHNIQGERLAHVLSGANVNF
HGLRYVSERCELGEQREALLAVTIPEQKGSFLRFCELLGGRSVTEFNYRYADAENACIFVGVRLTRGYAERVEILAELQD
KGYQVVDLSDDEMAKLHVRYMVGGRPSKPLRERLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFEL
SATEPQFEERLAALGYYCHDETDNPAFKFFLAG

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=297, Percent_Identity=32.6599326599327, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI19923959, Length=311, Percent_Identity=32.1543408360129, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI33469958, Length=314, Percent_Identity=27.0700636942675, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1790207, Length=514, Percent_Identity=84.6303501945525, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=288, Percent_Identity=37.5, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789235, Length=310, Percent_Identity=27.0967741935484, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71991565, Length=386, Percent_Identity=31.6062176165803, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537387, Length=314, Percent_Identity=33.4394904458599, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17508781, Length=288, Percent_Identity=32.2916666666667, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320930, Length=514, Percent_Identity=47.4708171206226, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322631, Length=304, Percent_Identity=33.8815789473684, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319788, Length=266, Percent_Identity=27.0676691729323, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355833, Length=319, Percent_Identity=32.6018808777429, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24645328, Length=251, Percent_Identity=29.8804780876494, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 55936; Mature: 55805

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSQPLSAAPCGAEYLRAILRAPVYEVAQVTPLQVMEKISSRVGNTVLVKREDRQPVHS
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHH
FKLRGAYAMISSLTEEQKACGVVTASAGNHAQGVALSAHKMGIKALIVMPVATADIKVDA
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEHHHCCCEEEEEEEEEECCEEEEE
VRAFGGEVLLFGANFDEAKTKAIALAQEQGYTFVPPFDHPAVIAGQGTLAMELLQQDAHL
HHHCCCEEEEEECCCCHHHHHHEEEEHHCCCEECCCCCCCEEEECCCHHHHHHHHHCCCC
DRVFVPVGGGGLVAGVAVLIKQLMPQIKVIGVEAEDSACLRAALDAGQPVDLARVGLFAE
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHC
GVAVKRIGDEPFRLCQEYLDDVITVDSDAICAAVKDLFEDVRAIAEPSGALALAGLKKYV
CEEEECCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QQHNIQGERLAHVLSGANVNFHGLRYVSERCELGEQREALLAVTIPEQKGSFLRFCELLG
HHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHC
GRSVTEFNYRYADAENACIFVGVRLTRGYAERVEILAELQDKGYQVVDLSDDEMAKLHVR
CCCEEECCEEECCCCCCEEEEEEEHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
YMVGGRPSKPLRERLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFE
EEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHC
LSATEPQFEERLAALGYYCHDETDNPAFKFFLAG
CCCCCHHHHHHHHHHHHEECCCCCCCEEEEEECC
>Mature Secondary Structure 
AVSQPLSAAPCGAEYLRAILRAPVYEVAQVTPLQVMEKISSRVGNTVLVKREDRQPVHS
CCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHH
FKLRGAYAMISSLTEEQKACGVVTASAGNHAQGVALSAHKMGIKALIVMPVATADIKVDA
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEHHHCCCEEEEEEEEEECCEEEEE
VRAFGGEVLLFGANFDEAKTKAIALAQEQGYTFVPPFDHPAVIAGQGTLAMELLQQDAHL
HHHCCCEEEEEECCCCHHHHHHEEEEHHCCCEECCCCCCCEEEECCCHHHHHHHHHCCCC
DRVFVPVGGGGLVAGVAVLIKQLMPQIKVIGVEAEDSACLRAALDAGQPVDLARVGLFAE
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHC
GVAVKRIGDEPFRLCQEYLDDVITVDSDAICAAVKDLFEDVRAIAEPSGALALAGLKKYV
CEEEECCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QQHNIQGERLAHVLSGANVNFHGLRYVSERCELGEQREALLAVTIPEQKGSFLRFCELLG
HHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHC
GRSVTEFNYRYADAENACIFVGVRLTRGYAERVEILAELQDKGYQVVDLSDDEMAKLHVR
CCCEEECCEEECCCCCCEEEEEEEHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEE
YMVGGRPSKPLRERLFSFEFPESPGALLKFLHTLGTHWNISLFHYRSHGTDFGRVLAAFE
EEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHC
LSATEPQFEERLAALGYYCHDETDNPAFKFFLAG
CCCCCHHHHHHHHHHHHEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]