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The map label for this gene is thiP [H]

Identifier: 170025758

GI number: 170025758

Start: 3896007

End: 3897614

Strand: Direct

Name: thiP [H]

Synonym: YPK_3543

Alternate gene names: 170025758

Gene position: 3896007-3897614 (Clockwise)

Preceding gene: 170025757

Following gene: 170025759

Centisome position: 83.08

GC content: 54.17

Gene sequence:

>1608_bases
ATGGCAATCCGCCGTCAGCCGTTAATCGCGCCCTGGCTATGGCCGGGGTTGCTGGCCGCTGGGCTGATTGTCGCGGTCGC
CTTATTGGCGTTCGCCGCTATTTGGCACCATGCGCCCACAGCAGATTGGCAAAGTGTCTGGCATGACAGTTATCTGTGGC
ATGTGATCCGCTTTACGTTCTGGCAGGCATTTTTATCCGCCCTGCTGTCGGTGATACCTGCTATCTTTCTGGCGCGGGCG
CTGTACCGCCGCAACTTCCCCGGCCGCCAGTTCATGCTGCGTTTATGTGCCATGACGCTGGTTCTGCCGGTGTTAGTCGC
GCTATTCGGCATTCTGACGGTCTATGGCCGTCAGGGGTGGCTGGCTCACCTGTTTGACTGGCTGGGTGTGGATTACCGCT
TCTCCCCCTATGGCTTGCAAGGTATTTTGTTAGCGCATATCTTCTTTAACCTACCACTGGCAACCCGCTTGTTGTTACAG
TCGCTAGAAGGCATCGCCGTGGAACAACGCCAACTGGCCGCACAACTGGGCATGAACGGCTGGCAGCATTTTCGCTGGGT
AGAGTGGCCTTATCTGCGTCGTCAAATCCTGCCCACTGCCGCCCTGATTTTTATGTTGTGCTTTGCCAGCTTTGCCACCG
TGTTGTCGCTGGGTGGTGGGCCACAGGCAACAACCATTGAACTGGCCATCTATCAGGCATTGAGCTACGACTATGATTTA
GGCCGTGCCGCATTATTGGCGCTGATACAACTGGGCTGCTGCCTGGGTTTAGTGGTACTCAGTCAGCGGCTGAACAGCGT
GTTGACGGTGGGCAATACGCATGGGCAAATATGGCGTAATCCGCAAGACAGCGGCTGGGCGCGCCTTGGCGATACCCTGC
TGATTTGTAGCGCATTACTCTTGATGGTACCGCCATTGCTAGCAGTTGTGATTGATGGCAGTAATAAAACAATACTGACC
GTGTTACAACAGCCCGCACTCTGGCAAGCGTTCTTTACCTCAGTGACTATCGCTATTGGTGCCGGGCTATTGTGTGTCAT
CTTAACCATGATGTTGCTCTGGAGTAGCCGCGAACTGAAATTACGCCAACGCGCGGCTTACGGTCAGGCATTGGAGGTCA
GCGGCATGGTTATTCTGGCGATGCCCGGCATTGTGTTGGCAACTGGTTTCTTCTTGTTGCTGAATGATACGCTGGGATTG
CCGCAATCTCCTTATGGGTTGGTGATCCTCACCAATGCCCTGATGGCCGTGCCCTACGCATTAAAAGTGCTGGATAACCC
GATGCGTGATCTGGCTGAGCGCTACACTCCGTTGTGTTTATCACTGGATATCCGTGGCTGGCACCGTTTACGTTATATCG
AGCTACGCGCCCTGAAACGCCCACTGGCTCAGGCGCTGGCCTTTGCCAGTGTGTTATCTATCGGCGATTTTGGCGTAGTC
GCGTTATTTGGTAATGAACACTTCCGCACCCTGCCATTTTATCTTTATCAACAGATAGGTTCCTACCGCAGCAATGATGG
CGCAGTGACTGCCCTGTTGCTTTTGTTGCTCTGCTTCCTGCTGTTTACCCTTATTGAACGACTGCCGAGCCGCCATGCTA
AAGCTTGA

Upstream 100 bases:

>100_bases
TGGATCTACCCGCCGGGTTCGAGACACTGACCGTGCCACAAACAGCGTTGCAATTTGATGCTAAAGACGTGGCGGATAAC
CGCAGTAAATGGATTCAGGC

Downstream 100 bases:

>100_bases
GAAAATCACCTATTTGTATGACCACTTACCCATGTGTTTTGATCTGCGCATTCAACCCGGTGAGCGGGTGGCGATCCTTG
GCCCAAGCGGCGCAGGTAAA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MAIRRQPLIAPWLWPGLLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDSYLWHVIRFTFWQAFLSALLSVIPAIFLARA
LYRRNFPGRQFMLRLCAMTLVLPVLVALFGILTVYGRQGWLAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQ
SLEGIAVEQRQLAAQLGMNGWQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
GRAALLALIQLGCCLGLVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALLLMVPPLLAVVIDGSNKTILT
VLQQPALWQAFFTSVTIAIGAGLLCVILTMMLLWSSRELKLRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGL
PQSPYGLVILTNALMAVPYALKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVV
ALFGNEHFRTLPFYLYQQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA

Sequences:

>Translated_535_residues
MAIRRQPLIAPWLWPGLLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDSYLWHVIRFTFWQAFLSALLSVIPAIFLARA
LYRRNFPGRQFMLRLCAMTLVLPVLVALFGILTVYGRQGWLAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQ
SLEGIAVEQRQLAAQLGMNGWQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
GRAALLALIQLGCCLGLVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALLLMVPPLLAVVIDGSNKTILT
VLQQPALWQAFFTSVTIAIGAGLLCVILTMMLLWSSRELKLRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGL
PQSPYGLVILTNALMAVPYALKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVV
ALFGNEHFRTLPFYLYQQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA
>Mature_534_residues
AIRRQPLIAPWLWPGLLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDSYLWHVIRFTFWQAFLSALLSVIPAIFLARAL
YRRNFPGRQFMLRLCAMTLVLPVLVALFGILTVYGRQGWLAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQS
LEGIAVEQRQLAAQLGMNGWQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDLG
RAALLALIQLGCCLGLVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALLLMVPPLLAVVIDGSNKTILTV
LQQPALWQAFFTSVTIAIGAGLLCVILTMMLLWSSRELKLRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGLP
QSPYGLVILTNALMAVPYALKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVVA
LFGNEHFRTLPFYLYQQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=534, Percent_Identity=74.1573033707865, Blast_Score=715, Evalue=0.0,
Organism=Escherichia coli, GI87082099, Length=208, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59663; Mature: 59532

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRRQPLIAPWLWPGLLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDSYLWHVIRFTF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
WQAFLSALLSVIPAIFLARALYRRNFPGRQFMLRLCAMTLVLPVLVALFGILTVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQSLEGIAVEQRQLAAQLGMNG
HHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCH
WQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCH
GRAALLALIQLGCCLGLVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LMVPPLLAVVIDGSNKTILTVLQQPALWQAFFTSVTIAIGAGLLCVILTMMLLWSSRELK
HHHHHHHEEEECCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGLPQSPYGLVILTNALMAVPYA
HHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
LKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVV
HHHHCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALFGNEHFRTLPFYLYQQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA
EEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AIRRQPLIAPWLWPGLLAAGLIVAVALLAFAAIWHHAPTADWQSVWHDSYLWHVIRFTF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
WQAFLSALLSVIPAIFLARALYRRNFPGRQFMLRLCAMTLVLPVLVALFGILTVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LAHLFDWLGVDYRFSPYGLQGILLAHIFFNLPLATRLLLQSLEGIAVEQRQLAAQLGMNG
HHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCCCH
WQHFRWVEWPYLRRQILPTAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCH
GRAALLALIQLGCCLGLVVLSQRLNSVLTVGNTHGQIWRNPQDSGWARLGDTLLICSALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LMVPPLLAVVIDGSNKTILTVLQQPALWQAFFTSVTIAIGAGLLCVILTMMLLWSSRELK
HHHHHHHEEEECCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LRQRAAYGQALEVSGMVILAMPGIVLATGFFLLLNDTLGLPQSPYGLVILTNALMAVPYA
HHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
LKVLDNPMRDLAERYTPLCLSLDIRGWHRLRYIELRALKRPLAQALAFASVLSIGDFGVV
HHHHCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALFGNEHFRTLPFYLYQQIGSYRSNDGAVTALLLLLLCFLLFTLIERLPSRHAKA
EEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]