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The map label for this gene is tbpA [H]

Identifier: 170025757

GI number: 170025757

Start: 3894955

End: 3896031

Strand: Direct

Name: tbpA [H]

Synonym: YPK_3542

Alternate gene names: 170025757

Gene position: 3894955-3896031 (Clockwise)

Preceding gene: 170025755

Following gene: 170025758

Centisome position: 83.06

GC content: 49.12

Gene sequence:

>1077_bases
ATGATAACGACTGTATCATCACGGATACCAATGTTAACTACCGCCTCAGATACCTTTGCCACCTTTATGTTCAAGGAGTG
CAAAGTGTTTAAACACATTATTTCCTGCTTATTACTGATATCAGCCACCTCCGCGCTTGCCGCAGAAAAACCTACGCTGA
CGGTCTATACCTACGACTCCTTTGCTGCTGACTGGGGCCCAGGCCCGGCGATTAAGCAGGCCTTTGAAGCTGAATGCGAT
TGCCAGCTAAAATTTGTGGCACTGGAAGATGGCGTTTCACTGCTGAACCGCCTGCGGATGGAAGGTAAAAACAGCCAGGC
CGATGTGATTTTAGGGTTGGATAACAATCTGGTACAGGCGGCAGAACAAACTGGCTTGTTTACCCCAAGCCCGGTTGATA
CCCGTAATCTGACCCTACCAGAGCCGTGGCAGAATAAGACATTTGTCCCTTACGATTATGGCTATTTTGCTTTTGTGTAT
AACAAAGAAAAACTGAAAAACCCGCCAAAAAGCCTGCACGAATTAATCAGCAGTAAAGAACCGTGGAAAGTGATTTATCA
AGACCCACGGACCAGCACTCCAGGTCTGGGTCTGATGCTGTGGATGCAAAAAGTTTATGGCGATCAAGCCCCGCAAGCAT
GGCAACAACTGGCGCAGAAAACGGTCACGGTCACCAAAGGCTGGAGCGAGGCGTATGGCTTGTTCCTCAAGGGGGAAGCG
GATTTAGTGCTGAGCTACACCACCTCTCCGGCTTATCATTTAATTGCAGAAAAGAATAACAACTATGCAGCTGCCGATTT
CAGTGAAGGCCACTATTTACAAGTAGAAGTCGCCGGCCAACTGGCCGCCAGCAAACAACCTGAACTGGCGCAGCGCTTTA
TGCAATTTATCGTGACCCCTGCGTTCCAAAACCACATTCCAACCGGCAACTGGATGTATCCAGTGATCAAAATGGATCTA
CCCGCCGGGTTCGAGACACTGACCGTGCCACAAACAGCGTTGCAATTTGATGCTAAAGACGTGGCGGATAACCGCAGTAA
ATGGATTCAGGCATGGCAATCCGCCGTCAGCCGTTAA

Upstream 100 bases:

>100_bases
AGACTGCTCAACACTGGAACCACCTAACCGCCGCAATTTTTTTCGCTGAGAGTAAACCCGTCGAACCTGATCCGGTTAAC
CCCGGCGGAGGGATTTGAGA

Downstream 100 bases:

>100_bases
TCGCGCCCTGGCTATGGCCGGGGTTGCTGGCCGCTGGGCTGATTGTCGCGGTCGCCTTATTGGCGTTCGCCGCTATTTGG
CACCATGCGCCCACAGCAGA

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MITTVSSRIPMLTTASDTFATFMFKECKVFKHIISCLLLISATSALAAEKPTLTVYTYDSFAADWGPGPAIKQAFEAECD
CQLKFVALEDGVSLLNRLRMEGKNSQADVILGLDNNLVQAAEQTGLFTPSPVDTRNLTLPEPWQNKTFVPYDYGYFAFVY
NKEKLKNPPKSLHELISSKEPWKVIYQDPRTSTPGLGLMLWMQKVYGDQAPQAWQQLAQKTVTVTKGWSEAYGLFLKGEA
DLVLSYTTSPAYHLIAEKNNNYAAADFSEGHYLQVEVAGQLAASKQPELAQRFMQFIVTPAFQNHIPTGNWMYPVIKMDL
PAGFETLTVPQTALQFDAKDVADNRSKWIQAWQSAVSR

Sequences:

>Translated_358_residues
MITTVSSRIPMLTTASDTFATFMFKECKVFKHIISCLLLISATSALAAEKPTLTVYTYDSFAADWGPGPAIKQAFEAECD
CQLKFVALEDGVSLLNRLRMEGKNSQADVILGLDNNLVQAAEQTGLFTPSPVDTRNLTLPEPWQNKTFVPYDYGYFAFVY
NKEKLKNPPKSLHELISSKEPWKVIYQDPRTSTPGLGLMLWMQKVYGDQAPQAWQQLAQKTVTVTKGWSEAYGLFLKGEA
DLVLSYTTSPAYHLIAEKNNNYAAADFSEGHYLQVEVAGQLAASKQPELAQRFMQFIVTPAFQNHIPTGNWMYPVIKMDL
PAGFETLTVPQTALQFDAKDVADNRSKWIQAWQSAVSR
>Mature_358_residues
MITTVSSRIPMLTTASDTFATFMFKECKVFKHIISCLLLISATSALAAEKPTLTVYTYDSFAADWGPGPAIKQAFEAECD
CQLKFVALEDGVSLLNRLRMEGKNSQADVILGLDNNLVQAAEQTGLFTPSPVDTRNLTLPEPWQNKTFVPYDYGYFAFVY
NKEKLKNPPKSLHELISSKEPWKVIYQDPRTSTPGLGLMLWMQKVYGDQAPQAWQQLAQKTVTVTKGWSEAYGLFLKGEA
DLVLSYTTSPAYHLIAEKNNNYAAADFSEGHYLQVEVAGQLAASKQPELAQRFMQFIVTPAFQNHIPTGNWMYPVIKMDL
PAGFETLTVPQTALQFDAKDVADNRSKWIQAWQSAVSR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=309, Percent_Identity=74.7572815533981, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40037; Mature: 40037

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTVSSRIPMLTTASDTFATFMFKECKVFKHIISCLLLISATSALAAEKPTLTVYTYDS
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
FAADWGPGPAIKQAFEAECDCQLKFVALEDGVSLLNRLRMEGKNSQADVILGLDNNLVQA
CCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHH
AEQTGLFTPSPVDTRNLTLPEPWQNKTFVPYDYGYFAFVYNKEKLKNPPKSLHELISSKE
HHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEHHHHCCCHHHHHHHHCCCC
PWKVIYQDPRTSTPGLGLMLWMQKVYGDQAPQAWQQLAQKTVTVTKGWSEAYGLFLKGEA
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCHHHCCEEEECCC
DLVLSYTTSPAYHLIAEKNNNYAAADFSEGHYLQVEVAGQLAASKQPELAQRFMQFIVTP
CEEEEEECCCEEEEEEECCCCEEEEECCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHCH
AFQNHIPTGNWMYPVIKMDLPAGFETLTVPQTALQFDAKDVADNRSKWIQAWQSAVSR
HHHCCCCCCCEEEEEEEEECCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITTVSSRIPMLTTASDTFATFMFKECKVFKHIISCLLLISATSALAAEKPTLTVYTYDS
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
FAADWGPGPAIKQAFEAECDCQLKFVALEDGVSLLNRLRMEGKNSQADVILGLDNNLVQA
CCCCCCCCHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHH
AEQTGLFTPSPVDTRNLTLPEPWQNKTFVPYDYGYFAFVYNKEKLKNPPKSLHELISSKE
HHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEHHHHCCCHHHHHHHHCCCC
PWKVIYQDPRTSTPGLGLMLWMQKVYGDQAPQAWQQLAQKTVTVTKGWSEAYGLFLKGEA
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCHHHCCEEEECCC
DLVLSYTTSPAYHLIAEKNNNYAAADFSEGHYLQVEVAGQLAASKQPELAQRFMQFIVTP
CEEEEEECCCEEEEEEECCCCEEEEECCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHCH
AFQNHIPTGNWMYPVIKMDLPAGFETLTVPQTALQFDAKDVADNRSKWIQAWQSAVSR
HHHCCCCCCCEEEEEEEEECCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]