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The map label for this gene is leuC [H]

Identifier: 170025752

GI number: 170025752

Start: 3888306

End: 3889736

Strand: Direct

Name: leuC [H]

Synonym: YPK_3537

Alternate gene names: 170025752

Gene position: 3888306-3889736 (Clockwise)

Preceding gene: 170025751

Following gene: 170025753

Centisome position: 82.92

GC content: 53.81

Gene sequence:

>1431_bases
ATGGGCACAACATCATCTCAGAGCAAAACGTTATACCAAAAATTGTACGATGCGCACATTGTGCATGAAGCCCCCAATGA
GACCCCATTGCTGTATATCGACCGCCATTTAGTGCATGAGGTCACCTCACCGCAAGCCTTTGATGGTTTGCGTGCCATGG
GCCGCCCGGTGCGTCAACCGGGTAAGACTTTCGCTACCATGGATCACAACGTTTCTACCCAAACAAAAGACATTAACGCC
AGTGGTGAGATGGCCCGTATTCAGATGCAAGAGTTAATTAAAAACTGCGCTGAATTCGGTGTCTCATTGTATGACCTGAA
CCACCCTTTCCAAGGGATCGTGCATGTCATTGGCCCTGAGCAAGGCATGACGTTACCGGGCATGACCATCGTTTGTGGTG
ACTCACATACCGCCACTCACGGCGCATTCGGCTCCTTGGCCTTTGGTATCGGCACCTCAGAAGTTGAGCATGTGTTGGCG
ACCCAAACCCTGAAGCAGGGACGAGCCAAAACCATGAGAATCGAAGTGAATGGCACTGTCGGCGCGGGTATCACGGCTAA
AGACATCGTGCTGGCGATTATCGGTAAAACCGGTAGCGCAGGCGGTACTGGTCATGTGGTGGAGTTCTGTGGCAGCGCGA
TTGAAGCATTGAGCATGGAAGGCCGGATGACATTATGCAACATGGCGATCGAAATGGGGGCCAAAGCCGGTTTGGTTGCC
CCTGATGACACCACCTTTGCCTATCTGAAAGGCCGTCAGTTCGCCCCAACTGGCGAGCAATGGGAACAAGGTGTTGCCTA
CTGGCGTACCCTGAAATCTGATGCTGATGCCCAATTCGATACCATCGTGACACTGGATGCCGCTGATATCGCGCCACAGG
TGACGTGGGGAACCAATCCGGGTCAGGTCATTGCGGTTAATCAAATCATTCCTGCCCCTGAGTCTTTCAGCGATCCGGTC
GAACGTGCATCAGCAGAGAAAGCCTTAGCTTATATGGACTTGCGCCCCGGCATTAAATTGACTGAAGTGGCGATTGATAA
AGTCTTTATCGGCTCTTGCACTAACTCACGTATTGAAGATTTACGTGCGGCAGCGGCTATTGCCCAAGGGCGTAAAGTCG
CCAAGGGTGTGCAAGCTATCGTGGTGCCAGGCTCCGGCCCGGTGAAAGCGCAGGCAGAAGCGGAAGGTTTGGATAAGATC
TTTATCGCCGCAGGTTTTGAGTGGCGTCTGCCCGGTTGCTCGATGTGTTTGGCCATGAATAATGACCGCCTGGAGCCCGG
CGAACGTTGTGCCTCCACCAGCAACCGTAATTTTGAAGGCCGCCAAGGCCGTGGGGGGCGCACACATTTGGTCAGCCCAG
CCATGGCCGCCGCTGCCGCCGTCAGTGGTCATTTTGCTGATGTTCGCGAATTATCCGCCACCACCCACTAA

Upstream 100 bases:

>100_bases
CAAGGTTATCGCACTGCGGATTTAGCCGGGGATGGTCACGCAATCGGCACTCACGAAATGGGCGATATCATCGCTAAATT
CGTGGTTGAGGGGGTATAAA

Downstream 100 bases:

>100_bases
CCGGAGACACCACCATGGCAAAATTTATTCAACACATTGGTTTGGTTGCGCCGTTAGATGCAGCAAACGTCGATACCGAC
GCGATTATCCCTAAGCAGTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPVRQPGKTFATMDHNVSTQTKDINA
SGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLA
TQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEALSMEGRMTLCNMAIEMGAKAGLVA
PDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWGTNPGQVIAVNQIIPAPESFSDPV
ERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGLDKI
FIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVSGHFADVRELSATTH

Sequences:

>Translated_476_residues
MGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPVRQPGKTFATMDHNVSTQTKDINA
SGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLA
TQTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEALSMEGRMTLCNMAIEMGAKAGLVA
PDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWGTNPGQVIAVNQIIPAPESFSDPV
ERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGLDKI
FIAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVSGHFADVRELSATTH
>Mature_475_residues
GTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPVRQPGKTFATMDHNVSTQTKDINAS
GEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPEQGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLAT
QTLKQGRAKTMRIEVNGTVGAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEALSMEGRMTLCNMAIEMGAKAGLVAP
DDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWGTNPGQVIAVNQIIPAPESFSDPVE
RASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIEDLRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGLDKIF
IAAGFEWRLPGCSMCLAMNNDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVSGHFADVRELSATTH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=412, Percent_Identity=25.9708737864078, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=26.5151515151515, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI41352693, Length=386, Percent_Identity=24.6113989637306, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=85.8064516129032, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=486, Percent_Identity=24.8971193415638, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367097, Length=375, Percent_Identity=27.4666666666667, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI32564738, Length=412, Percent_Identity=27.4271844660194, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25149337, Length=412, Percent_Identity=27.4271844660194, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=28.3387622149837, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17568399, Length=464, Percent_Identity=26.0775862068966, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=476, Percent_Identity=59.2436974789916, Blast_Score=582, Evalue=1e-167,
Organism=Saccharomyces cerevisiae, GI6320440, Length=423, Percent_Identity=26.9503546099291, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=413, Percent_Identity=26.634382566586, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=405, Percent_Identity=27.1604938271605, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281365315, Length=411, Percent_Identity=24.8175182481752, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17864292, Length=411, Percent_Identity=24.8175182481752, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076999, Length=411, Percent_Identity=24.8175182481752, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571643, Length=411, Percent_Identity=25.3041362530414, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645686, Length=455, Percent_Identity=26.5934065934066, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17137564, Length=455, Percent_Identity=24.6153846153846, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50590; Mature: 50459

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPVRQP
CCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCC
GKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPE
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHEEEECCC
QGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMRIEVNGTV
CCCCCCCEEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEALSMEGRMTLCNMAIEMGAKAGLVA
CCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
PDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWGTNP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHCCCEEEECCCC
GQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIED
CCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEECCCCCEEEHEEHHHEEEECCCCHHHHH
LRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGLDKIFIAAGFEWRLPGCSMCLAMN
HHHHHHHHHCHHHHCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCEEECCCCEEEEEEC
NDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVSGHFADVRELSATTH
CCCCCHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
GTTSSQSKTLYQKLYDAHIVHEAPNETPLLYIDRHLVHEVTSPQAFDGLRAMGRPVRQP
CCCCCHHHHHHHHHHHHHHEECCCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCC
GKTFATMDHNVSTQTKDINASGEMARIQMQELIKNCAEFGVSLYDLNHPFQGIVHVIGPE
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHEEEECCC
QGMTLPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQGRAKTMRIEVNGTV
CCCCCCCEEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GAGITAKDIVLAIIGKTGSAGGTGHVVEFCGSAIEALSMEGRMTLCNMAIEMGAKAGLVA
CCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
PDDTTFAYLKGRQFAPTGEQWEQGVAYWRTLKSDADAQFDTIVTLDAADIAPQVTWGTNP
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHCCCEEEECCCC
GQVIAVNQIIPAPESFSDPVERASAEKALAYMDLRPGIKLTEVAIDKVFIGSCTNSRIED
CCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEECCCCCEEEHEEHHHEEEECCCCHHHHH
LRAAAAIAQGRKVAKGVQAIVVPGSGPVKAQAEAEGLDKIFIAAGFEWRLPGCSMCLAMN
HHHHHHHHHCHHHHCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCEEECCCCEEEEEEC
NDRLEPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVSGHFADVRELSATTH
CCCCCHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA