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The map label for this gene is pcnB [H]

Identifier: 170025685

GI number: 170025685

Start: 3812096

End: 3813571

Strand: Direct

Name: pcnB [H]

Synonym: YPK_3470

Alternate gene names: 170025685

Gene position: 3812096-3813571 (Clockwise)

Preceding gene: 170025684

Following gene: 170025686

Centisome position: 81.29

GC content: 52.37

Gene sequence:

>1476_bases
GTGCTGATCCGCGAAGACAAAACGGTCCGTGATGACAAGGCCCGTAAAGATAAAGTGCCCGGTGAAGATAACGTGGCCCG
CAAAGAGAGAAGGCCCGCCAGAGCACATACTGGCCGCAAGGGTCACGCGGTGTCGTCTTCGGAACAGCGCCAAATGGCGA
TTATTCCGCGGGATCAGCACAATATCTCCCGCAGAGATATCAGTGACAATGCACTGAAGGTTCTCTATCGCCTGAATAAA
TCAGGTTACGAAGCTTATCTGGTGGGCGGCGGTGTCCGCGACCTGCTACTGGGCAGAAAACCCAAAGATTTCGATATCAC
CACCAGCGCCACCCCTGAACAGGTGCGAAAACTGTTCCGTAACTGTCGTTTGGTTGGTCGCCGCTTCCGTTTGGCTCATG
TGATGTTTGGCCCTGAAATTATTGAAGTAGCGACTTTCCGTGGCCACCACGAGCAGCAACAAGCAGAAGACAGCGATAAG
AACTCCTCCCAGCAGGCGCAAAATGGCATGTTGCTGCGCGACAATATCTTTGGCTCCATCGAAGATGATGCTCAACGCCG
TGATTTCACCATTAACAGCCTCTACTACGGTATTTCTGATTTTGCGCTGCGTGACTACACCGGCGGCTTACGTGATCTGA
AAGAGGGTATTATTCGTCTAATTGGCGACCCAGAAACCCGTTACCGTGAAGATCCGGTACGGATGCTGCGTGCGGTTCGT
TTTGCCGCAAAACTGGATATGTCAATCAGCCCGGAAACGGCAGAGCCTATACCGCGTCTGGCCTCACTGTTGCGCGAAAT
CCCGCCCGCCCGCCTGTTTGAGGAGTCACTCAAGCTGCTGCAATCCGGCTACGGCTATAAAACCTATTTAAAACTGTGCG
AATACCAGCTTTTCCAGCCGCTATTCCCACTGATTGCCCGTAATTTTACTGAACAGCATGATTCGCCAATGGAGCGTATT
CTGGTTCAGGTACTGAAAAATACCGACCACCGTCTGCATAATGATCAGCGGGTTAATCCTGCCTTCTTATTTGCGGCGAT
GCTGTGGTATCCGCTGATTGAACATGCGCAGAAGTTAACGCAGGAAAGCGGCCTGGCCTATTACGATGCATTTGCATTGG
CCATGAATGATGTACTGGACGAAGAGTGCCGCTCACTGGCGATCCCTAAACGTATCACCTCACTGGTACGGGATATCTGG
TTGTTACAACTGCGTCTGTCACGTCGTCAGGGGAAACGTGCCCATAAGCTGATGGAACACCCTAAATTCCGGGCGGCTTA
CGATCTGTTACTCCTACGGGCAGAAGTGGAAAAAAATCACGAGCTGCAACGTTTGGCTCAGTGGTGGGGCGAATTCCAGG
AAGCCACTCCCACGCAACAGAAATCCATGCTGAATACATTAGGTGCTGATCCTGCGCCACGGCGTTCACGTCCACGCCGC
CCACGCAAGCCAGTACCCCGTAAAGAAGGGGTATAA

Upstream 100 bases:

>100_bases
GGTGAGCTATGATTAGCCGCTGTTTTTCTGTCGTTCTGTTATTTATTAGCCACTATCGAGGTGTACCATTTTTACCCGAG
TAGCCAATTTCTGCCGTAAG

Downstream 100 bases:

>100_bases
TTTTACCATGATCCGGGTCTATATCGCGCTGGGTAGCAATTTGGCCATGCCACTACAACAGGTTAGCGCGGCACGGGAAG
CCTTGGCGCATCTGCCGCGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQHNISRRDISDNALKVLYRLNK
SGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDK
NSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQPLFPLIARNFTEQHDSPMERI
LVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIW
LLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
PRKPVPRKEGV

Sequences:

>Translated_491_residues
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQHNISRRDISDNALKVLYRLNK
SGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDK
NSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQPLFPLIARNFTEQHDSPMERI
LVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIW
LLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
PRKPVPRKEGV
>Mature_491_residues
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQHNISRRDISDNALKVLYRLNK
SGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDK
NSSQQAQNGMLLRDNIFGSIEDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQPLFPLIARNFTEQHDSPMERI
LVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLTQESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIW
LLQLRLSRRQGKRAHKLMEHPKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
PRKPVPRKEGV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI87081691, Length=417, Percent_Identity=80.0959232613909, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=230, Percent_Identity=30.8695652173913, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=191, Percent_Identity=28.2722513089005, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281360112, Length=276, Percent_Identity=27.8985507246377, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357337, Length=276, Percent_Identity=27.8985507246377, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 56925; Mature: 56925

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQH
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCCEEEECCCCCC
NISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFR
CCCHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
NCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSI
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCEEEECCCCCCC
EDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
CCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHH
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQP
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLT
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEH
HHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
PRKPVPRKEGV
CCCCCCCCCCC
>Mature Secondary Structure
MLIREDKTVRDDKARKDKVPGEDNVARKERRPARAHTGRKGHAVSSSEQRQMAIIPRDQH
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCCEEEECCCCCC
NISRRDISDNALKVLYRLNKSGYEAYLVGGGVRDLLLGRKPKDFDITTSATPEQVRKLFR
CCCHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
NCRLVGRRFRLAHVMFGPEIIEVATFRGHHEQQQAEDSDKNSSQQAQNGMLLRDNIFGSI
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCEEEECCCCCCC
EDDAQRRDFTINSLYYGISDFALRDYTGGLRDLKEGIIRLIGDPETRYREDPVRMLRAVR
CCHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHH
FAAKLDMSISPETAEPIPRLASLLREIPPARLFEESLKLLQSGYGYKTYLKLCEYQLFQP
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LFPLIARNFTEQHDSPMERILVQVLKNTDHRLHNDQRVNPAFLFAAMLWYPLIEHAQKLT
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QESGLAYYDAFALAMNDVLDEECRSLAIPKRITSLVRDIWLLQLRLSRRQGKRAHKLMEH
HHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PKFRAAYDLLLLRAEVEKNHELQRLAQWWGEFQEATPTQQKSMLNTLGADPAPRRSRPRR
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCC
PRKPVPRKEGV
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]