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The map label for this gene is rbsB [H]

Identifier: 170025602

GI number: 170025602

Start: 3715826

End: 3716767

Strand: Reverse

Name: rbsB [H]

Synonym: YPK_3387

Alternate gene names: 170025602

Gene position: 3716767-3715826 (Counterclockwise)

Preceding gene: 170025606

Following gene: 170025601

Centisome position: 79.26

GC content: 50.32

Gene sequence:

>942_bases
ATGAAAAATACCCTACTGAAATCCTGTCTTACCGCAGCATTAATCTCAATGGCAGGTGTTGCTGGTGCGGCCAGCAATGG
TCTGATCGCCATCATCACCCCCTCCCACGATAATCCGTTCTTTAAAGCAGAGGCTGAAGGTGCCAAAGCGAAAGCGACGG
AACTGGGATACACCACACTGGTTGCTTCGCATGATGACGATGTGAACAAACAGAACCAACTCATTGAGACCGCTATCGCC
CGTAAAGCCAAAGCGATCATTCTGGATAACGCCGGCTCTGACGCGACCATCGGGCCATTGAAAAAAGCCAAAGCCGCGGG
TATCCCGGCCTTCCTTATTGACCGTGAAATCAATGAAACCGGGATTGCCGTCTCTCAGATTGTCTCTAACAACTATCAGG
GCGCGCAGCTGGGCGCGGAGAAGTTTGTCACTCTGATGGGGGGCAAAGGTAAATATATTGAATTACTGGGGCGCGAGTCT
GACACCAACGCTCATGTACGTTCGCAAGGTTATCACGACGTCATTGACGAACATAGCGACATGAAGATGGTTGCTCAACA
GACAGCAAACTGGAGCCAGACTGAAGCATTCAACCGTATGGAATCCATTTTGCAGGCTAACCCTGATATTACAGGGGTCA
TTTCCGGTAACGACACGATGGCATTAGGGGCCGAAGCCGCATTAAAAGCGGCGGGCCGCAATGATGTGGTGGTGGTGGGA
TTTGATGGCAGTGACTATGTCCGCGACTCGATCATTAACAACGGCAATATCAAAGCCACCGTATTGCAGCCGGGTTGGGC
GCAAGCACAAATGGCCGTTGTCCAGGCCGATCAGTATCTGAAAACCGGTAAAACCGGTCTGGAAGAGAAACAACTGATGG
ACTGTGTATTGATTGACGAAAATAACGCTAAGAACCTGAATGTCTTTGCGCTGAAAGACTAG

Upstream 100 bases:

>100_bases
TACTTTCCTTGCTCTGACATTGGACAAGGCGTAGGGCGTTTGTATTATTAGATTGAATATAAATTTAATAGTGAATATAT
ATTCAATGCGGAGAAAAATA

Downstream 100 bases:

>100_bases
CCGCTACAGGCGTGTTCGCTAAAGCCAGTGCGATAAAACATCCGCTTTAGCCGTAATGAGGGGCGACATCGCCCCTCCTT
TCAGGAGGCATTATGAGGTT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTLVASHDDDVNKQNQLIETAIA
RKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRES
DTNAHVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGAEAALKAAGRNDVVVVG
FDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDENNAKNLNVFALKD

Sequences:

>Translated_313_residues
MKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTLVASHDDDVNKQNQLIETAIA
RKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRES
DTNAHVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGAEAALKAAGRNDVVVVG
FDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDENNAKNLNVFALKD
>Mature_313_residues
MKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTLVASHDDDVNKQNQLIETAIA
RKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINETGIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRES
DTNAHVRSQGYHDVIDEHSDMKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGAEAALKAAGRNDVVVVG
FDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDENNAKNLNVFALKD

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=293, Percent_Identity=31.0580204778157, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790526, Length=244, Percent_Identity=26.6393442622951, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790715, Length=261, Percent_Identity=25.6704980842912, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790674, Length=210, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33250; Mature: 33250

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTL
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCHHHCCEEEE
VASHDDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINET
EECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCC
GIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRESDTNAHVRSQGYHDVIDEHSD
CHHHHHHHHCCCCCHHHCHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCH
MKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGAEAALKAAGRNDVVVVG
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECHHHHHHHCCCCCEEEEE
FDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDE
ECCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEC
NNAKNLNVFALKD
CCCCEEEEEEECC
>Mature Secondary Structure
MKNTLLKSCLTAALISMAGVAGAASNGLIAIITPSHDNPFFKAEAEGAKAKATELGYTTL
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCHHHCCEEEE
VASHDDDVNKQNQLIETAIARKAKAIILDNAGSDATIGPLKKAKAAGIPAFLIDREINET
EECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCC
GIAVSQIVSNNYQGAQLGAEKFVTLMGGKGKYIELLGRESDTNAHVRSQGYHDVIDEHSD
CHHHHHHHHCCCCCHHHCHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCH
MKMVAQQTANWSQTEAFNRMESILQANPDITGVISGNDTMALGAEAALKAAGRNDVVVVG
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECHHHHHHHCCCCCEEEEE
FDGSDYVRDSIINNGNIKATVLQPGWAQAQMAVVQADQYLKTGKTGLEEKQLMDCVLIDE
ECCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEC
NNAKNLNVFALKD
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]