Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 170025588

GI number: 170025588

Start: 3699043

End: 3701670

Strand: Reverse

Name: alaS [H]

Synonym: YPK_3373

Alternate gene names: 170025588

Gene position: 3701670-3699043 (Counterclockwise)

Preceding gene: 170025589

Following gene: 170025587

Centisome position: 78.94

GC content: 50.15

Gene sequence:

>2628_bases
ATGAGCAAGAGCACCGCTGAGATCCGTCAAGCGTTTCTCGATTTCTTCCATAGCAAGGGACACCAGGTTGTATCAAGCAG
CTCTTTGGTCCCTAATAATGACCCTACGCTGTTGTTTACCAATGCCGGGATGAACCAGTTTAAGGACGTTTTCTTAGGTT
TGGATAAACGGGCCTATTCCCGTGCGACGACCTCTCAGCGCTGCGTGCGAGCGGGTGGTAAGCATAACGACCTGGAAAAC
GTAGGTTATACCGCGCGTCATCATACTTTCTTCGAAATGTTGGGTAACTTCAGCTTCGGCGATTACTTCAAGCATGATGC
TATCAACTTCGCTTGGGAGTTACTGACCAGTGAACAGTGGTTTAATTTACCAAAAGAAAAACTTTGGGTAACGGTGTACG
AGACCGATGACGAGGCCTATAACATCTGGGCTAACGAAGTCGGCGTTCCTCATGAGCGTATCATCCGTATCGGTGATAAC
AAAGGGGGGGCCTTTGCCTCAGATAACTTCTGGCAAATGGGTGATACCGGCCCTTGTGGCCCATGTTCAGAGATTTTCTT
TGACCATGGTGATCATATCTGGGGTGGTCCTCCAGGGTCTGCAGAAGAAGATGGCGATCGCTATATCGAGATCTGGAATA
TCGTCTTTATGCAGTTTAACCGTCAGTCTGACGGTACGATGCTGCCATTGCCGAAGCCATCGGTTGATACCGGCATGGGG
CTGGAACGTATTGCTGCGGTATTACAGCATGTTAACTCCAACTATGAGATTGACCTGTTCCGTGATTTGATTGCCGCAGT
CGCAGATGTCACGGGGGCGACGGATTTATCCAGCAAGTCGTTGCGGGTTATTGCTGATCACATTCGTTCTTGTGCTTTCT
TGATTTCAGATGGTGTGATCCCATCGAATGAAAACCGCGGCTATGTCTTACGCCGTATTATTCGCCGTGCAATTCGCCAC
GGGAATATGTTGGGTGCGAAAGAGACTTTCTTCTACAAACTGGTGGCTCCCTTGATCGCGGTTATGGGGCCTGCTGCTGC
TGAGTTAAAACAACAGCAAGCGATGGTAGAGCAAGTATTAAAGACGGAAGAAGAGCAGTTTGCCAGGACGTTAGAGCGCG
GTTTGGCGCTGCTTGATGACGAGCTCAGCAAGCTTACCGGCGATACCCTTGATGGGGAAACGGCCTTCCGCTTGTACGAT
ACCTATGGCTTCCCAGTTGATCTAACGGCAGATGTTTGTCGCGAACGTAACCTGAAAGTCGATGAAGCTGGCTTTGAGCA
AGCGATGGAAGCTCAACGTCGCCGTGCCCGTGAATCCAGTGGTTTTGGCGCAGATTACAACAGCTTGATCCGTGTTGATA
GCGCAAGCCAATTTTCTGGTTATGACCATGTCCAACAACACGCGACGGTTACTGCTCTATTCCGTAATGGCGAGGCCGTT
GATGAGATTCACGCAGGTGAAGAAGCCGTTGTCGTACTGAATCGGACACCATTCTACGGTGAATCGGGTGGTCAGGTCGG
CGATAAGGGGGAACTGAAAAATGCGACAGCAACCTTTAGCGTTACTGATACTCAGAAATATGGTCAGGCTATCGGGCACG
TAGGAATATTGACTACCGGCACGTTAAGGGTTAACCACAGCGTCGAAGCATTGGTTGATGTTGTGCGTCGTAACCGTATC
CGTCTGAATCACTCCGCGACCCACTTACTGCATGCTGCGTTGCGTAACGTCTTGGGCGAGCATGTGGCCCAGAAGGGGTC
ACTGGTTAATGACAAATATCTACGCTTTGATTTCTCTCATTTCGAAGCAATGAAGCCGGAACAAATCCGGTTGGTAGAAG
ACCTGGTCAATGAACAAATCCGCCGTAACATGCCGGTTCAGACCGAAGTGATGGAACTGGATGCGGCGAAAGAAAAAGGT
GCAATGGCACTGTTCGGCGAGAAGTATGACGACCAAGTACGTGTTCTGACCATGGGTGACTTCTCAACAGAGTTGTGTGG
GGGGACTCATGCTAGCCGCACGGGGGATATAGGGTTATTCCGCATCCTGACCGAATCTGGCACGGCTGCGGGTATTCGTC
GTATTGAAGCCGTCACTGGCGAGGGCGCGATTGCTTTATTGCATCAGCAAAGCGACCTGTTACAGGACGTCGCTCATCTG
GTGAAAGGTGATATTCATAATCTGGCAGACAAAGTCCGGGCGGTATTGGATCGCAGTAAAATGCTTGAGCGTGAGTTGCA
ACAGTTGAAAGATCAGCAGGCAGCTCAGGAAAGTGCCTCGTTGTCTTCAAGCGCGAAATTGATCAATGGTGTGAAGCTTT
TGGTCAGTCAGTTGGATAACGTTGAACCAAAAATGCTGCGGACAATGGTCGATGACCTTAAAAATCAACTGGGTTCTGCC
ATTATTGTATTGGCCACCACAGCTGATGATAAGGTAAGTTTGATTGTTGGGGTGACCAAGGACCTGACAGGTAAAGTAAA
AGCGGGTGAATTAATCGCGGACATTGCTCAGCAGGTGGGCGGTAAAGGCGGTGGTCGTCCTGATATGGCTCAGGCGGGGG
GTACTGATGTGCAGGCTTTGCCAGCGGCATTAGCCAGCGTAGAAGCATGGGTAGCATCCCGGATGTAA

Upstream 100 bases:

>100_bases
CTTATTCCAACTTTTGTTCGTGAGCTTATCCATTGCGTTGTCGCTAATCTGTACACGATTTGCTCACGGGCCATCCTTTT
AGCTTGATTTCAGGACAATT

Downstream 100 bases:

>100_bases
GTAATCATCTATTAAATCAACACGCCATATCTCGGTACTAGGTATGGCGTTTTTTAATTTTCCTGTCATAGTTCAATCAC
AAAGTTAAACGCAAAGTTGT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MSKSTAEIRQAFLDFFHSKGHQVVSSSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQWFNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDN
KGGAFASDNFWQMGDTGPCGPCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVIPSNENRGYVLRRIIRRAIRH
GNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVLKTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYD
TYGFPVDLTADVCRERNLKVDEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTGTLRVNHSVEALVDVVRRNRI
RLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKG
AMALFGEKYDDQVRVLTMGDFSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDNVEPKMLRTMVDDLKNQLGSA
IIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVGGKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM

Sequences:

>Translated_875_residues
MSKSTAEIRQAFLDFFHSKGHQVVSSSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQWFNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDN
KGGAFASDNFWQMGDTGPCGPCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVIPSNENRGYVLRRIIRRAIRH
GNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVLKTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYD
TYGFPVDLTADVCRERNLKVDEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTGTLRVNHSVEALVDVVRRNRI
RLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKG
AMALFGEKYDDQVRVLTMGDFSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDNVEPKMLRTMVDDLKNQLGSA
IIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVGGKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM
>Mature_874_residues
SKSTAEIRQAFLDFFHSKGHQVVSSSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQWFNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDNK
GGAFASDNFWQMGDTGPCGPCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMGL
ERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVIPSNENRGYVLRRIIRRAIRHG
NMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVLKTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYDT
YGFPVDLTADVCRERNLKVDEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAVD
EIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTGTLRVNHSVEALVDVVRRNRIR
LNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKGA
MALFGEKYDDQVRVLTMGDFSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHLV
KGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDNVEPKMLRTMVDDLKNQLGSAI
IVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVGGKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=988, Percent_Identity=37.6518218623482, Blast_Score=550, Evalue=1e-156,
Organism=Homo sapiens, GI38569417, Length=971, Percent_Identity=33.9855818743563, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1789048, Length=875, Percent_Identity=82.5142857142857, Blast_Score=1509, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=977, Percent_Identity=35.7215967246674, Blast_Score=526, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17536681, Length=756, Percent_Identity=32.8042328042328, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324911, Length=809, Percent_Identity=41.7799752781211, Blast_Score=574, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24582809, Length=979, Percent_Identity=36.7722165474974, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI45552267, Length=979, Percent_Identity=36.7722165474974, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24658214, Length=824, Percent_Identity=30.3398058252427, Blast_Score=342, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96318; Mature: 96187

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSTAEIRQAFLDFFHSKGHQVVSSSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYS
CCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHH
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHH
FNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDNKGGAFASDNFWQMGDTGPCG
HCCCCHHEEEEEEECCCCCEEEEHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
CHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEEEEHHEECCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAIRHGNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYDTYGFPVDLTADVCRERNLKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
CHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCHHCCCHHHHHHHHHHEEHCCCCCHH
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTG
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEEC
TLRVNHSVEALVDVVRRNRIRLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSH
EEEECCCHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHH
FEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKGAMALFGEKYDDQVRVLTMGD
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECC
FSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
CCHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDN
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVG
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSTAEIRQAFLDFFHSKGHQVVSSSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYS
CCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHH
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAINFAWELLTSEQW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHH
FNLPKEKLWVTVYETDDEAYNIWANEVGVPHERIIRIGDNKGGAFASDNFWQMGDTGPCG
HCCCCHHEEEEEEECCCCCEEEEHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCSEIFFDHGDHIWGGPPGSAEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG
CHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEEEEHHEECCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYEIDLFRDLIAAVADVTGATDLSSKSLRVIADHIRSCAFLISDGVI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAIRHGNMLGAKETFFYKLVAPLIAVMGPAAAELKQQQAMVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDDELSKLTGDTLDGETAFRLYDTYGFPVDLTADVCRERNLKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEQAMEAQRRRARESSGFGADYNSLIRVDSASQFSGYDHVQQHATVTALFRNGEAV
CHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCHHCCCHHHHHHHHHHEEHCCCCCHH
DEIHAGEEAVVVLNRTPFYGESGGQVGDKGELKNATATFSVTDTQKYGQAIGHVGILTTG
HHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEEC
TLRVNHSVEALVDVVRRNRIRLNHSATHLLHAALRNVLGEHVAQKGSLVNDKYLRFDFSH
EEEECCCHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHH
FEAMKPEQIRLVEDLVNEQIRRNMPVQTEVMELDAAKEKGAMALFGEKYDDQVRVLTMGD
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECC
FSTELCGGTHASRTGDIGLFRILTESGTAAGIRRIEAVTGEGAIALLHQQSDLLQDVAHL
CCHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHH
VKGDIHNLADKVRAVLDRSKMLERELQQLKDQQAAQESASLSSSAKLINGVKLLVSQLDN
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIVGVTKDLTGKVKAGELIADIAQQVG
CCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDVQALPAALASVEAWVASRM
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA