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The map label for this gene is yafJ [H]

Identifier: 170025524

GI number: 170025524

Start: 3620425

End: 3621198

Strand: Reverse

Name: yafJ [H]

Synonym: YPK_3307

Alternate gene names: 170025524

Gene position: 3621198-3620425 (Counterclockwise)

Preceding gene: 170025525

Following gene: 170025522

Centisome position: 77.22

GC content: 50.0

Gene sequence:

>774_bases
ATGTGCGAATTGCTTGGGATGAGCGCAAATGTGCCCACGGATATTTGCTTTAGCTTTACCGGCCTGGTGCAGCGTGGTGG
GGGGACTGGCCCGCATAAAGATGGCTGGGGGATTACTTTCTATGAAGGTAATGGCTGCCGCACGTTTAAAGACCCTCAAC
CCAGTTTTAATTCGCCGATTGCCCGCTTGGTGCAAGATTACCCGATAAAATCCTGTGCAGTGGTTTCCCATATCCGTCAA
GCAAATCGGGGGGAAGTCGCGTTAGAAAATACCCATCCCTTCACCCGTGAAATGTGGGGGCGTAACTGGACCTATGCGCA
TAATGGCCAGCTTAAGGGGTACCGCCAATTGGATACCGGGACTTTTCGGCCCATCGGCCAGACCGATAGCGAATATGCTT
TCTGCTGGCTACTCAATCAATTAGCAAAACGCTACCCTCGTACCCCCGGTAACTGGCCTGCGGTATTCCGCTTTATTGCT
AGTTTATCCGAGCAATTACGGGCCAAAGGGGTATTTAATATGTTGCTATCCGATGGGCGTTTTGTGATGGCATATTGCTC
AACTAATCTCTACTGGATAACCCGCCGTGCGCCTTTTGGCAAAGCCACGTTGCTGGATCAAGATGTTGAGATCGATTTTC
AGCAGCAGACCACACCTAAAGATGTGGTCACGGTAATTGCTACCCAGCCACTGACCGGTAATGAAACCTGGCACAAAATT
GCACCAGGTGAATTTGCTTTATTCTGTCTTGGTGAGCGGGTCGGTTTTAATTAG

Upstream 100 bases:

>100_bases
TTGCTGGCCTGGTTGGATATTAGTTTACTGTTTGTCTATTAGATGACGGCTCGTTTATCAGAGGGCTGGTCATGTTTCAG
ATAAGTCGCGGAGGTTGGTT

Downstream 100 bases:

>100_bases
TGCCAATTGCGATGTTGGCGTACTAGTGAGCGGTGGCGGGCTTAACACATACTGCCCGTTGCTGACTGAAACGGGCGGTG
GCATCCGGTTTTTGGCAAAA

Product: glutamine amidotransferase class-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGNGCRTFKDPQPSFNSPIARLVQDYPIKSCAVVSHIRQ
ANRGEVALENTHPFTREMWGRNWTYAHNGQLKGYRQLDTGTFRPIGQTDSEYAFCWLLNQLAKRYPRTPGNWPAVFRFIA
SLSEQLRAKGVFNMLLSDGRFVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPKDVVTVIATQPLTGNETWHKI
APGEFALFCLGERVGFN

Sequences:

>Translated_257_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGNGCRTFKDPQPSFNSPIARLVQDYPIKSCAVVSHIRQ
ANRGEVALENTHPFTREMWGRNWTYAHNGQLKGYRQLDTGTFRPIGQTDSEYAFCWLLNQLAKRYPRTPGNWPAVFRFIA
SLSEQLRAKGVFNMLLSDGRFVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPKDVVTVIATQPLTGNETWHKI
APGEFALFCLGERVGFN
>Mature_257_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGNGCRTFKDPQPSFNSPIARLVQDYPIKSCAVVSHIRQ
ANRGEVALENTHPFTREMWGRNWTYAHNGQLKGYRQLDTGTFRPIGQTDSEYAFCWLLNQLAKRYPRTPGNWPAVFRFIA
SLSEQLRAKGVFNMLLSDGRFVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPKDVVTVIATQPLTGNETWHKI
APGEFALFCLGERVGFN

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=86.2204724409449, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28976; Mature: 28976

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGNGCRTFKDPQPSFNSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
ARLVQDYPIKSCAVVSHIRQANRGEVALENTHPFTREMWGRNWTYAHNGQLKGYRQLDTG
HHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCEEECCCC
TFRPIGQTDSEYAFCWLLNQLAKRYPRTPGNWPAVFRFIASLSEQLRAKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPKDVVTVIATQPLTGNETWHKI
EEEEEECCCEEEEEECCCCCCCEEECCCCCCCCCCCCCHHHHEEEEECCCCCCCCCEEEE
APGEFALFCLGERVGFN
CCCCEEEEEECHHCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGNGCRTFKDPQPSFNSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
ARLVQDYPIKSCAVVSHIRQANRGEVALENTHPFTREMWGRNWTYAHNGQLKGYRQLDTG
HHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCEEECCCC
TFRPIGQTDSEYAFCWLLNQLAKRYPRTPGNWPAVFRFIASLSEQLRAKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVMAYCSTNLYWITRRAPFGKATLLDQDVEIDFQQQTTPKDVVTVIATQPLTGNETWHKI
EEEEEECCCEEEEEECCCCCCCEEECCCCCCCCCCCCCHHHHEEEEECCCCCCCCCEEEE
APGEFALFCLGERVGFN
CCCCEEEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]