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The map label for this gene is queA [H]

Identifier: 170025482

GI number: 170025482

Start: 3569618

End: 3570688

Strand: Reverse

Name: queA [H]

Synonym: YPK_3265

Alternate gene names: 170025482

Gene position: 3570688-3569618 (Counterclockwise)

Preceding gene: 170025485

Following gene: 170025481

Centisome position: 76.14

GC content: 51.07

Gene sequence:

>1071_bases
ATGCGTGTTGCCGATTTTTCTTTTGAGCTACCGGAAGCTCTGATTGCCCATTACCCTCAGCCTCAGCGCAGTGGTTGCCG
CTTGTTATCGCTGGATGGCCCTACGGGGACGTTAACGCACGGCATTTTCACCGATTTGCTGGATAAGCTGGCACCGGGTG
ATCTGCTGGTGTTCAATAATACTCGGGTGATACCGGCCCGTTTATTTGGTCGTAAAGCCAGTGGTGGCAAGCTCGAAGTG
CTGGTCGAGCGTGTTTTGGATGACCATCGGGTATTGGCTCATGTCAAAGCCTCTAAAGCACCGAAACCGGGCGCAGAACT
GCTATTGGGCGATGATGAAAATATCCGGGCAACCATGTTGGCGCGGCATGACACATTGTTTGAGTTGCGTTTTGACGATG
AGCGGGATGTTTTCACCATCCTAAATGCAGTCGGTCATATGCCATTGCCTCCTTATATTGATCGTCCTGATGAAGATGCT
GACCGCGAACTGTATCAAACGGTTTATAGCCAACGTCCGGGCGCGGTTGCAGCACCAACTGCGGGCCTACACTTCGATGA
GCCAATGCTGGCGACACTGCAGGAAAAAGGCATTGAGATGGCTTTTGTGACACTGCATGTTGGCGCGGGGACTTTCCAGC
CAGTGCGTGTCGATACCATCGAAGACCACATCATGCACTCTGAATATGCAGAAGTCCCGCAAGAGGTGGTTGATGCGGTT
CTGGCCTGTAAAGCCCGTGGCAAGCGTGTAGTCGCTGTTGGTACGACATCGGTACGTTCGTTGGAAAGTGCAGCGAAGGC
CGCTGAAAACGGGTTAATTGCGCCTTTCTTCGGTGATACCCGCATCTTCATTTATCCCGGTTATCACTATCAGGTTGTGG
ATGCGTTAGTGACTAACTTCCACCTACCTGAATCAACATTGATAATGTTGGTCTCTGCTTTTGCAGGTTACAAAAACACC
ATGAATGCTTATCAGCAGGCAGTGGCAGAACAATATCGTTTTTTCAGTTACGGCGATGCCATGTTCATCAGCCGTAATCC
ACGGGCACCGCAAGAGAAGGTTAGCCCGTAA

Upstream 100 bases:

>100_bases
AGGCTGTTTCTTACCATTTTGTGTACTATTGGTGCCCAAAATAGTTTGCAGCAACACGGCCCTGTTGGTGTGGGTTTGGT
CTTAGATGAAGTGGAAAGCC

Downstream 100 bases:

>100_bases
ATTTCGGTCATACAGGTTGTGTTTTTTCAACGACATAAGCGTGTGATCAGGCAAAAACAACATCAGACTGTTTCTCTGAT
GCTGGAGGTTATGTGAAGTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRVADFSFELPEALIAHYPQPQRSGCRLLSLDGPTGTLTHGIFTDLLDKLAPGDLLVFNNTRVIPARLFGRKASGGKLEV
LVERVLDDHRVLAHVKASKAPKPGAELLLGDDENIRATMLARHDTLFELRFDDERDVFTILNAVGHMPLPPYIDRPDEDA
DRELYQTVYSQRPGAVAAPTAGLHFDEPMLATLQEKGIEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
LACKARGKRVVAVGTTSVRSLESAAKAAENGLIAPFFGDTRIFIYPGYHYQVVDALVTNFHLPESTLIMLVSAFAGYKNT
MNAYQQAVAEQYRFFSYGDAMFISRNPRAPQEKVSP

Sequences:

>Translated_356_residues
MRVADFSFELPEALIAHYPQPQRSGCRLLSLDGPTGTLTHGIFTDLLDKLAPGDLLVFNNTRVIPARLFGRKASGGKLEV
LVERVLDDHRVLAHVKASKAPKPGAELLLGDDENIRATMLARHDTLFELRFDDERDVFTILNAVGHMPLPPYIDRPDEDA
DRELYQTVYSQRPGAVAAPTAGLHFDEPMLATLQEKGIEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
LACKARGKRVVAVGTTSVRSLESAAKAAENGLIAPFFGDTRIFIYPGYHYQVVDALVTNFHLPESTLIMLVSAFAGYKNT
MNAYQQAVAEQYRFFSYGDAMFISRNPRAPQEKVSP
>Mature_356_residues
MRVADFSFELPEALIAHYPQPQRSGCRLLSLDGPTGTLTHGIFTDLLDKLAPGDLLVFNNTRVIPARLFGRKASGGKLEV
LVERVLDDHRVLAHVKASKAPKPGAELLLGDDENIRATMLARHDTLFELRFDDERDVFTILNAVGHMPLPPYIDRPDEDA
DRELYQTVYSQRPGAVAAPTAGLHFDEPMLATLQEKGIEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
LACKARGKRVVAVGTTSVRSLESAAKAAENGLIAPFFGDTRIFIYPGYHYQVVDALVTNFHLPESTLIMLVSAFAGYKNT
MNAYQQAVAEQYRFFSYGDAMFISRNPRAPQEKVSP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=83.0508474576271, Blast_Score=618, Evalue=1e-178,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39381; Mature: 39381

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFSFELPEALIAHYPQPQRSGCRLLSLDGPTGTLTHGIFTDLLDKLAPGDLLVFNN
CCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECC
TRVIPARLFGRKASGGKLEVLVERVLDDHRVLAHVKASKAPKPGAELLLGDDENIRATML
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEE
ARHDTLFELRFDDERDVFTILNAVGHMPLPPYIDRPDEDADRELYQTVYSQRPGAVAAPT
ECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCC
AGLHFDEPMLATLQEKGIEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LACKARGKRVVAVGTTSVRSLESAAKAAENGLIAPFFGDTRIFIYPGYHYQVVDALVTNF
HHHHCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYKNTMNAYQQAVAEQYRFFSYGDAMFISRNPRAPQEKVSP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MRVADFSFELPEALIAHYPQPQRSGCRLLSLDGPTGTLTHGIFTDLLDKLAPGDLLVFNN
CCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECC
TRVIPARLFGRKASGGKLEVLVERVLDDHRVLAHVKASKAPKPGAELLLGDDENIRATML
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEE
ARHDTLFELRFDDERDVFTILNAVGHMPLPPYIDRPDEDADRELYQTVYSQRPGAVAAPT
ECCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCC
AGLHFDEPMLATLQEKGIEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQEVVDAV
CCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LACKARGKRVVAVGTTSVRSLESAAKAAENGLIAPFFGDTRIFIYPGYHYQVVDALVTNF
HHHHCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYKNTMNAYQQAVAEQYRFFSYGDAMFISRNPRAPQEKVSP
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA