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The map label for this gene is tgt [H]

Identifier: 170025481

GI number: 170025481

Start: 3568401

End: 3569525

Strand: Reverse

Name: tgt [H]

Synonym: YPK_3264

Alternate gene names: 170025481

Gene position: 3569525-3568401 (Counterclockwise)

Preceding gene: 170025482

Following gene: 170025480

Centisome position: 76.12

GC content: 48.89

Gene sequence:

>1125_bases
GTGAAGTACGAATTACAAAAAACTGATGGGCGCGCCCGCCGTGGTCGGCTGGTGTTTGAGCGTGGCGTGGTCGAAACCCC
GGCCTTTATGCCCGTTGGGACGTATGGCACCGTGAAAGGGATGACACCGGAAGAAGTAAAAGAGACCGGTGCGCAGATCC
TGTTAGGTAACACCTTCCATCTATGGTTGCGTCCAGGCCAGGAAATCATGAAGCTGCACGGCGACTTACATGATTTCATG
CAATGGCACGGCCCGATCCTGACCGATTCCGGCGGGTTCCAGGTCTTTAGCCTTGGCGCGATGCGTAAAATTAAAGAAGA
AGGGGTTCATTTTAAAAACCCAATCAATGGCGATTCGGTGTTCCTTAGCCCTGAAAAGTCGATGGAAATTCAGTACGATC
TCGGTTCTGATATCGTCATGATATTTGATGAGTGTACACCTTATCCTGCCGATTGGGATTACGCTAAACGTTCGATGGAA
ATGTCACTGCGCTGGGCTGCCCGTAGCCGCCAGCGCTTTGATGAGCTGAATAACAAGAATGCATTATTCGGTATCATCCA
AGGCGGGGTTTACGAAGATTTACGTGATGTGTCAGTAAAAGGGCTGGTAGATATCGGCTTTGATGGTTACGCTGTTGGCG
GCTTGGCGGTCGGTGAACCAAAAGAAGATATGCACCGTATCCTTGAACATGTCTGCCCACAGATCCCAGAAGATAAACCT
CGTTACCTGATGGGCGTAGGGAAACCCGAGGATCTGGTTGAGGGGGTGCGCCGTGGTATTGACATGTTTGATTGTGTCAT
GCCAACGCGTAATGCCCGAAATGGTCATTTGTTTGTGACTGATGGTGTGGTAAAAATCCGTAATGCGAAGCATAAGGATG
ATACGGCAACACTGGATGAGCACTGTGATTGCTACACGTGTCGCCATTATAGCCGTGCCTACTTGCATCATCTCGATCGT
TGCAATGAAATACTGGGTGCACGATTGAACACTATTCATAACCTGCGTTATTACCAGCGCTTAATGGCGGGTTTACGTCA
GGCTATTGAAGAGGGTAAATTAGAACACTTTGTTGAAGATTTCTATGGTCGGATCGGGAAGCCAGTTCCACCATTAAACG
TTTGA

Upstream 100 bases:

>100_bases
GCCCGTAAATTTCGGTCATACAGGTTGTGTTTTTTCAACGACATAAGCGTGTGATCAGGCAAAAACAACATCAGACTGTT
TCTCTGATGCTGGAGGTTAT

Downstream 100 bases:

>100_bases
TTCTAAAATTATTGTCTTTGTATTTATAGAGAGAGCAGTTACGATTTGTCGCGGCTGTTTTATCGCACTGATTTCACACT
ACTGATATTGATAACAATCG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKYELQKTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFM
QWHGPILTDSGGFQVFSLGAMRKIKEEGVHFKNPINGDSVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAARSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTATLDEHCDCYTCRHYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVEDFYGRIGKPVPPLNV

Sequences:

>Translated_374_residues
MKYELQKTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFM
QWHGPILTDSGGFQVFSLGAMRKIKEEGVHFKNPINGDSVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAARSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTATLDEHCDCYTCRHYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVEDFYGRIGKPVPPLNV
>Mature_374_residues
MKYELQKTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFHLWLRPGQEIMKLHGDLHDFM
QWHGPILTDSGGFQVFSLGAMRKIKEEGVHFKNPINGDSVFLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAARSRQRFDELNNKNALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTATLDEHCDCYTCRHYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVEDFYGRIGKPVPPLNV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=373, Percent_Identity=42.3592493297587, Blast_Score=298, Evalue=6e-81,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=89.572192513369, Blast_Score=703, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=375, Percent_Identity=41.3333333333333, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=171, Percent_Identity=32.1637426900585, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920510, Length=363, Percent_Identity=44.6280991735537, Blast_Score=310, Evalue=8e-85,
Organism=Drosophila melanogaster, GI21355311, Length=269, Percent_Identity=28.2527881040892, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42633; Mature: 42633

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYELQKTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFH
CCCCCCCCCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGAMRKIKEEGVHFKNPINGDSV
EEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCEE
FLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAARSRQRFDELNNKN
EECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
EEEEEECCCHHHHHHHCCCCCEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTATLDE
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCHHH
HCDCYTCRHYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVED
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYGRIGKPVPPLNV
HHHHCCCCCCCCCC
>Mature Secondary Structure
MKYELQKTDGRARRGRLVFERGVVETPAFMPVGTYGTVKGMTPEEVKETGAQILLGNTFH
CCCCCCCCCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGAMRKIKEEGVHFKNPINGDSV
EEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCEE
FLSPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAARSRQRFDELNNKN
EECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ALFGIIQGGVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP
EEEEEECCCHHHHHHHCCCCCEEEECCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKHKDDTATLDE
CEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCHHH
HCDCYTCRHYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVED
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYGRIGKPVPPLNV
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA