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The map label for this gene is gne [H]

Identifier: 170025396

GI number: 170025396

Start: 3475619

End: 3476578

Strand: Reverse

Name: gne [H]

Synonym: YPK_3179

Alternate gene names: 170025396

Gene position: 3476578-3475619 (Counterclockwise)

Preceding gene: 170025397

Following gene: 170025395

Centisome position: 74.14

GC content: 33.12

Gene sequence:

>960_bases
ATGAAAATAGCTTTGATTGGTGGTTCTGGTTTTATTGGGACAAATTTGGCCCGATTATTAATAGATAATAGTGTTGATTT
TTCTATTTTAGATAAAGTTAAAAGTGATGTATATCCTGAGCGATGGGTATATTGTGATGTCACAGATTATGATAGTTTAA
TATCTACTCTTATCGGTCATGATTTAATTATAAATTTGGCTGCAGAACATAAAGATAATGTTAATCCTATTAGTCTCTAC
TATCAAGTTAATGTTGAGGGAGCTAAAAATATTTGTAGGGCTGCGGATAGCCTTAATATAAAGAATATTGTATTTACATC
TTCTGTAGCTGTTTACGGTTTTGTTGAAAAAGATACAGATGAAAGTGGAAAATACGCTCCTTTCAATCATTATGGAAAAT
CTAAGTTAGAAGCAGAAAAGGTCTATGATTCTTGGTTTAATTCTTCTGCAGACAAAAAACTAGTTACTCTTCGTCCCACG
GTTGTTTTTGGTATTGGCAACAGAGGCAATGTATATAATTTATTTAAGCAAATTGCATCAGGCAAGTTTGTTATGATTGG
TCGTGGTGAGAACGAGAAATCTATGGCCTACGTGGAGAACATAGCTGCTTTCTTAGTTCTTACTCTATCATTTCCTGCTG
GATATCATTTAATAAACTATGTGGATAAGCCAGACTTCACTATGAATGAGCTAGCTAATGTCATTTATACATGTCTCGGG
AAAAAGAGTAAGATCGTTAGAGTCCCTTATTTTTTTGGTCTCTTTGCAGGTTATATATTTGATCTGTTGGCGAAAATTAC
TGGCAAAGAGTTACCTGTAAGTAGTATTCGAATTAAAAAATTCTGCGCTAAAACACAGTTTAGTTCAAAAAATATTGAGA
ATTATAAATTTTCGGCTCCTTACTCTTTACAAGACGCTGTAGTGAAAACAATCTCACAAGAGTTTATGAGTGAGAAGTAA

Upstream 100 bases:

>100_bases
TTTACTACGATGTAGTACTGGTGAAAAGAAAAATTGATGTTTTAGATATTCTATCATTAAGTCACTACTGATTTACTCTA
AAGTCATAAAGAGAATATAT

Downstream 100 bases:

>100_bases
TGTTAATATAATAACTTGGGAGGATAATATTTAATTTTAAAGTTGGTTTTAATTCCTTTATGAGATAATGCTTTTATATT
TTTTATAAAATTCCGGCACA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKIALIGGSGFIGTNLARLLIDNSVDFSILDKVKSDVYPERWVYCDVTDYDSLISTLIGHDLIINLAAEHKDNVNPISLY
YQVNVEGAKNICRAADSLNIKNIVFTSSVAVYGFVEKDTDESGKYAPFNHYGKSKLEAEKVYDSWFNSSADKKLVTLRPT
VVFGIGNRGNVYNLFKQIASGKFVMIGRGENEKSMAYVENIAAFLVLTLSFPAGYHLINYVDKPDFTMNELANVIYTCLG
KKSKIVRVPYFFGLFAGYIFDLLAKITGKELPVSSIRIKKFCAKTQFSSKNIENYKFSAPYSLQDAVVKTISQEFMSEK

Sequences:

>Translated_319_residues
MKIALIGGSGFIGTNLARLLIDNSVDFSILDKVKSDVYPERWVYCDVTDYDSLISTLIGHDLIINLAAEHKDNVNPISLY
YQVNVEGAKNICRAADSLNIKNIVFTSSVAVYGFVEKDTDESGKYAPFNHYGKSKLEAEKVYDSWFNSSADKKLVTLRPT
VVFGIGNRGNVYNLFKQIASGKFVMIGRGENEKSMAYVENIAAFLVLTLSFPAGYHLINYVDKPDFTMNELANVIYTCLG
KKSKIVRVPYFFGLFAGYIFDLLAKITGKELPVSSIRIKKFCAKTQFSSKNIENYKFSAPYSLQDAVVKTISQEFMSEK
>Mature_319_residues
MKIALIGGSGFIGTNLARLLIDNSVDFSILDKVKSDVYPERWVYCDVTDYDSLISTLIGHDLIINLAAEHKDNVNPISLY
YQVNVEGAKNICRAADSLNIKNIVFTSSVAVYGFVEKDTDESGKYAPFNHYGKSKLEAEKVYDSWFNSSADKKLVTLRPT
VVFGIGNRGNVYNLFKQIASGKFVMIGRGENEKSMAYVENIAAFLVLTLSFPAGYHLINYVDKPDFTMNELANVIYTCLG
KKSKIVRVPYFFGLFAGYIFDLLAKITGKELPVSSIRIKKFCAKTQFSSKNIENYKFSAPYSLQDAVVKTISQEFMSEK

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Escherichia coli, GI1786974, Length=216, Percent_Identity=29.6296296296296, Blast_Score=61, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321437, Length=349, Percent_Identity=25.7879656160458, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.7 [H]

Molecular weight: Translated: 35705; Mature: 35705

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALIGGSGFIGTNLARLLIDNSVDFSILDKVKSDVYPERWVYCDVTDYDSLISTLIGH
CEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCC
DLIINLAAEHKDNVNPISLYYQVNVEGAKNICRAADSLNIKNIVFTSSVAVYGFVEKDTD
HHEEEEHHHCCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCC
ESGKYAPFNHYGKSKLEAEKVYDSWFNSSADKKLVTLRPTVVFGIGNRGNVYNLFKQIAS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCHHHHHHHHHC
GKFVMIGRGENEKSMAYVENIAAFLVLTLSFPAGYHLINYVDKPDFTMNELANVIYTCLG
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHC
KKSKIVRVPYFFGLFAGYIFDLLAKITGKELPVSSIRIKKFCAKTQFSSKNIENYKFSAP
CCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCC
YSLQDAVVKTISQEFMSEK
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIALIGGSGFIGTNLARLLIDNSVDFSILDKVKSDVYPERWVYCDVTDYDSLISTLIGH
CEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCC
DLIINLAAEHKDNVNPISLYYQVNVEGAKNICRAADSLNIKNIVFTSSVAVYGFVEKDTD
HHEEEEHHHCCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCC
ESGKYAPFNHYGKSKLEAEKVYDSWFNSSADKKLVTLRPTVVFGIGNRGNVYNLFKQIAS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECEEEEECCCCCCHHHHHHHHHC
GKFVMIGRGENEKSMAYVENIAAFLVLTLSFPAGYHLINYVDKPDFTMNELANVIYTCLG
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHC
KKSKIVRVPYFFGLFAGYIFDLLAKITGKELPVSSIRIKKFCAKTQFSSKNIENYKFSAP
CCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCC
YSLQDAVVKTISQEFMSEK
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796 [H]