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The map label for this gene is manB [H]

Identifier: 170025395

GI number: 170025395

Start: 3473942

End: 3475315

Strand: Reverse

Name: manB [H]

Synonym: YPK_3178

Alternate gene names: 170025395

Gene position: 3475315-3473942 (Counterclockwise)

Preceding gene: 170025396

Following gene: 170025394

Centisome position: 74.11

GC content: 38.72

Gene sequence:

>1374_bases
ATGCCAAAACTAACCTGTTTTAAAGCCTATGATATTCGTGGTCGATTGGGTGATGAATTGAATGAAGATATAGCTTACCG
TATTGGTCGCGCGTATGGTGAGTTTGCTAAACCCAAAAAGGTTGTAGTAGGGGGGGATGTTCGATTAACCAGTGAAAGTC
TTAAGTTAGCGTTAAGTCAAGGTTTACAAGATGCAGGTACTGATGTATTAGATATTGGTTTGTCAGGCACTGAGGAGATA
TATTTTTCCACCTTCCATCTTGGGGTTGATGGTGGTATAGAAGTGACTGCCAGCCATAACCCAATAGACTACAATGGAAT
GAAGTTAGTGCGTGAAGGCTCCAGACCTATCAGTGGAGATACAGGGTTACGGGATATTCAGTATTTAGCTGAACAGAATG
AATTCCCTCCAGTGGACAGTAAAAAAAGAGGTGGTTATAAAAAAATATCTATTATTAATGACTATGTTGAACACTTAATT
AACTATATTAATCCTTCTCTTTTTAAACCGTTGAAATTAGTTGTAAATACTGGCAATGGTGCTGCTGGTCATGTTATTGA
TGCTTTAGAAAAACGTTTTTCAGAATTAAACATACCGATAACTCTGATTAAAATTCATCATGAGCCAGATGGCACATTCC
CGAATGGTATTCCGAATCCACTGTTACCAGAATGCCGCCAGGATACTACTGATGCTGTGCTGATGCATGACGCTGATATG
GGTATTGCCTTTGATGGCGATTTTGACCGTTGTTTCTTCTTTAATAATAAGGGTGAGTTTATTGAAGGCTATTATATCGT
GGGGCTTTTAGCCGAAGCTTTTCTTCAAAAAAATGCTGGAGCTAAAATTATCCATGATCCACGTTTAAGCTGGAATACTA
TTGATATAGTTAATTCTTCTGGTGGAACGCCTATAATGTCCAAAACCGGGCACGCTTTTATTAAAGAACGCATGCGAGAA
GAAGATGCGGTTTATGGTGGCGAAATGAGTGCTCATCACTACTTCCGCGACTTTGCTTATTGTGATTCAGGGATGATTCC
ATGGTTACTGATTGCTGAGCTTTTATGTGTCAAAGAAAAAAGTTTGGATCAGTTAGTTGAAGAGCGGATGCGTATTTACC
CTGCATCTGGAGAAATTAATTCTAAGTTAACCGATCCAACCTCTGCGATAGCGCGTGTTCGTGCCGAATATGAAGTTAAG
GCTATAGCTATTGACGAAACTGATGGTATTAGTATGGAATTTGATGGGTGGCGTTTTAATTTACGCAGTTCAAATACTGA
ACCTGTTGTTCGGCTTAATGTGGAGTCACATTACAATAAGACATTAATGCATCATAAAACAGAAGAGATACTTTCTCTAT
TAAGAAAAGAATAA

Upstream 100 bases:

>100_bases
TATTGAATCCTACATAATGCATAAAGTAATGAGAGTTTCTGGGCTATGTCGTTTTTATGCAAAAACTTATCTAAACTTCC
TCAGTTTTTAAAGTGATCTT

Downstream 100 bases:

>100_bases
TTATTTTAGATGGCATTCCTAGCATTACTATATATAGGTAATACTCAATGAGTAATAAACAGGCACGAAGTAACCTTGAT
AGTCCAATACCGAATAACTA

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MPKLTCFKAYDIRGRLGDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQGLQDAGTDVLDIGLSGTEEI
YFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGDTGLRDIQYLAEQNEFPPVDSKKRGGYKKISIINDYVEHLI
NYINPSLFKPLKLVVNTGNGAAGHVIDALEKRFSELNIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSSGGTPIMSKTGHAFIKERMRE
EDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEKSLDQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVK
AIAIDETDGISMEFDGWRFNLRSSNTEPVVRLNVESHYNKTLMHHKTEEILSLLRKE

Sequences:

>Translated_457_residues
MPKLTCFKAYDIRGRLGDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQGLQDAGTDVLDIGLSGTEEI
YFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGDTGLRDIQYLAEQNEFPPVDSKKRGGYKKISIINDYVEHLI
NYINPSLFKPLKLVVNTGNGAAGHVIDALEKRFSELNIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSSGGTPIMSKTGHAFIKERMRE
EDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEKSLDQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVK
AIAIDETDGISMEFDGWRFNLRSSNTEPVVRLNVESHYNKTLMHHKTEEILSLLRKE
>Mature_456_residues
PKLTCFKAYDIRGRLGDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQGLQDAGTDVLDIGLSGTEEIY
FSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGDTGLRDIQYLAEQNEFPPVDSKKRGGYKKISIINDYVEHLIN
YINPSLFKPLKLVVNTGNGAAGHVIDALEKRFSELNIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADMG
IAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSSGGTPIMSKTGHAFIKERMREE
DAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEKSLDQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVKA
IAIDETDGISMEFDGWRFNLRSSNTEPVVRLNVESHYNKTLMHHKTEEILSLLRKE

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O9 antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=454, Percent_Identity=74.2290748898678, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=447, Percent_Identity=27.0693512304251, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 51143; Mature: 51012

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLTCFKAYDIRGRLGDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQ
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECHHHHHHHHHH
GLQDAGTDVLDIGLSGTEEIYFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGD
CHHHHCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCCCCCCC
TGLRDIQYLAEQNEFPPVDSKKRGGYKKISIINDYVEHLINYINPSLFKPLKLVVNTGNG
CCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHCCCEEEEEECCCC
AAGHVIDALEKRFSELNIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCEEEEEECCC
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSS
CEEECCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
GGTPIMSKTGHAFIKERMREEDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLDQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVKAIAIDETDGISMEFDGWRFN
HHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCEEEEEEEEECCCCCEEEECCEEEE
LRSSNTEPVVRLNVESHYNKTLMHHKTEEILSLLRKE
ECCCCCCCEEEEEEHHCCCHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
PKLTCFKAYDIRGRLGDELNEDIAYRIGRAYGEFAKPKKVVVGGDVRLTSESLKLALSQ
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECHHHHHHHHHH
GLQDAGTDVLDIGLSGTEEIYFSTFHLGVDGGIEVTASHNPIDYNGMKLVREGSRPISGD
CHHHHCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCCCCCCC
TGLRDIQYLAEQNEFPPVDSKKRGGYKKISIINDYVEHLINYINPSLFKPLKLVVNTGNG
CCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCHHHCCCEEEEEECCCC
AAGHVIDALEKRFSELNIPITLIKIHHEPDGTFPNGIPNPLLPECRQDTTDAVLMHDADM
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCEEEEEECCC
GIAFDGDFDRCFFFNNKGEFIEGYYIVGLLAEAFLQKNAGAKIIHDPRLSWNTIDIVNSS
CEEECCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECC
GGTPIMSKTGHAFIKERMREEDAVYGGEMSAHHYFRDFAYCDSGMIPWLLIAELLCVKEK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SLDQLVEERMRIYPASGEINSKLTDPTSAIARVRAEYEVKAIAIDETDGISMEFDGWRFN
HHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCEEEEEEEEECCCCCEEEECCEEEE
LRSSNTEPVVRLNVESHYNKTLMHHKTEEILSLLRKE
ECCCCCCCEEEEEEHHCCCHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7515042; 9004408 [H]