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The map label for this gene is tesA [H]

Identifier: 170025378

GI number: 170025378

Start: 3454913

End: 3455485

Strand: Direct

Name: tesA [H]

Synonym: YPK_3159

Alternate gene names: 170025378

Gene position: 3454913-3455485 (Clockwise)

Preceding gene: 170025375

Following gene: 170025379

Centisome position: 73.67

GC content: 52.88

Gene sequence:

>573_bases
TTGCGTGCCGCCGCAACCGATACACTATTGATTCTCGGCGACAGTTTAAGCGCCGGTTACCGCCTGCCTATTGCACAGGC
ATGGCCTTCGCTATTGGATAAAAAATGGCAAGCCACGCCTTCGTTACCGAAGGTGGTCAATGCCAGTATCAGTGGAGATA
CCGCCGCGCAAGGGCTAGCGCGCTTACCCGCCCTACTCAAGCAACATCAGCCACGCTGGGTGCTGATTGAACTGGGTGCC
AATGACGCGCTGCGCGGGTTTCCGACGCAGGACATTCAACGGGATCTGAGCGAAATTATCAATCAGATAACCGCAGCGAA
AGCCCAGCCATTATTGATGCAAATCCGTATTCCCCCCAACTATGGCCGCCGTTATACTGATGCTTTCACCGCTATCTATC
CACAACTGGCGCAACAGTTCGACATCCCCCTACTCCCTTTTTTTATGGAACAGGTTGCCGTTAAGCCAGAGTGGATGCAG
GATGATGGTTTACACCCTAACGGCGATGCCCAACCGTTTATCGCGGATTGGATGGCGCAACAACTCAAGCCGTTAGTTGT
TGATCCGAAATAA

Upstream 100 bases:

>100_bases
GCTGTTGCTCACCCTGACCGACGTGCTTATTAAGATGATGAACTTCAAGAACGTTTTCCGCTGGCATCTTCCCTTCCTTT
TACTGTTGGGATTATTCAGT

Downstream 100 bases:

>100_bases
ATATGATTTAAACATCGCTCAGACTGTTAGCCCTGTTTTGACAGGTTAATCAGGCTAGAAAAAAGGGAAAGCGCACTAAA
AGTGCGGTAATCCCTTCGCT

Product: multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGLARLPALLKQHQPRWVLIELGA
NDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWMQ
DDGLHPNGDAQPFIADWMAQQLKPLVVDPK

Sequences:

>Translated_190_residues
MRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGLARLPALLKQHQPRWVLIELGA
NDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWMQ
DDGLHPNGDAQPFIADWMAQQLKPLVVDPK
>Mature_190_residues
MRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGLARLPALLKQHQPRWVLIELGA
NDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPNYGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWMQ
DDGLHPNGDAQPFIADWMAQQLKPLVVDPK

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=186, Percent_Identity=70.9677419354839, Blast_Score=279, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21118; Mature: 21118

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGLA
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHH
RLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPN
HHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
YGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWMQDDGLHPNGDAQPFIADWMAQ
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
QLKPLVVDPK
HCCCCCCCCC
>Mature Secondary Structure
MRAAATDTLLILGDSLSAGYRLPIAQAWPSLLDKKWQATPSLPKVVNASISGDTAAQGLA
CCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHH
RLPALLKQHQPRWVLIELGANDALRGFPTQDIQRDLSEIINQITAAKAQPLLMQIRIPPN
HHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
YGRRYTDAFTAIYPQLAQQFDIPLLPFFMEQVAVKPEWMQDDGLHPNGDAQPFIADWMAQ
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
QLKPLVVDPK
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]