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The map label for this gene is mrdA [H]

Identifier: 170025242

GI number: 170025242

Start: 3322083

End: 3323978

Strand: Direct

Name: mrdA [H]

Synonym: YPK_3021

Alternate gene names: 170025242

Gene position: 3322083-3323978 (Clockwise)

Preceding gene: 170025241

Following gene: 170025243

Centisome position: 70.84

GC content: 49.47

Gene sequence:

>1896_bases
ATGAAAAAAGAACCTAACCCTTTTCGCGACTATTCGGCTGAATCAGCCCTGTTTGTCCGCCGCGCCGTAGTGGCTTTTCT
GGGTATCCTGCTACTGAGCGGGGTACTGGTTGCTAACCTCTATAATTTGCAAATAGTCCGTTTCGACGATTACAGCACCC
GCTCCAACGACAACCGCATCAAACTGGTTCCTATCGCCCCTAGCCGTGGCATGATATTTGACAGAAATGGCACACCACTG
GCATTAAACCGCACCATCTATCAATTAGAGTTGATGCCGGAAAAGATCGAAAACTTGTCAGCCACCCTCAATGCGCTGCG
CCCAATAGTGGATCTGACTGACGACGATATCGCTAACTTTGAAAAAGAGCGCAAACGCTCACGTCGCTTTACCTCTATTG
CCGTTAAAACACCGTTGACCGAGGTGCAAGTCGCCCGTTTTGCAGTGAATCAATTCCGCTTCCCCGGAATTGAAGTCAAA
GGCTATCAACGTCGCTTCTACCCGTATGGCTCCGCCCTGACCCACGTGATTGGCTATGTCTCTAAAATTAACGATAAAGA
TGTCGAACGGTTGGATAAAGAAGGCATTCTCCCCAATTATGCTGCGACCCACGATATCGGTAAGTTGGGTATTGAACGCT
ATTATGAATCCACCTTACATGGCAAAACTGGTTATGAAGAAGTTGAAGTCAATAACCGAGGCCGGGTAATCCGCCAATTA
CATGAGCAACCGCCACAAGCAGGTAAAGATATTTATCTGACTCTCGATCTGAGTCTACAAATCTATATCGAGAAGTTACT
CAGTGGCAGCCGTGCTGCGGTCGTCGTCACAGACCCACGCACCGGCGGGATCCTGGCCTTGGTATCAAACCCAAGCTATG
ACCCGAACCTGTTTGTCGACGGCATCTCCAACAAAGATTATCAAGGGTTGCTTAACGATACGAACCGCCCCTTAATCAAT
CGGGCAACCCAAGGCGTTTATCCACCGGCATCAACCGTGAAACCGTACATCGCAGTGTCAGCACTGAGTGCCGGGGTTAT
CACCAAAAACACCAGCCTGTTCGATCCTGGTTGGTGGCAACTGCCCGGTTCGGAAAAGCGCTACCGTGACTGGAAAAAAT
GGGGCCATGGCCGCCTGAACGTCACCAAAGCACTGGAAGAATCGGCAGATACCTTCTTCTACCAAGTCGCCTATGATATG
GGCATTGATCGCTTATCATCTTGGATGAGCAAATTCGGGTACGGTGAATATACTGGCATTGATTTATCCGAAGAGCGTGC
GGGTTTAATGCCAACACGCGAGTGGAAGCAAAAGCGCCATAAAAAGCCTTGGTATCAGGGTGATACCATTCCGGTAGGTA
TTGGCCAGGGTTATTGGACTGCGACGCCAATCCAGATGGCTAAAGCCTTAATGACCCTGATTAATGATGGCACAGTCAAG
ACACCTCACCTGTTGCAAAGCACCCGGATTGATGGCGTCTTAGTGCCTTATAAGCAGGAAGACAGTACGCAGATTGGGAG
TATCAATTCTGGCTATTGGGAAATTGCTAAAGACGGCATGTATGGCGTAGCGAACCGCCCGAACGGTACTGGCCGCAAGT
TTTTTGAAGGTACACCTTATAAGGCAGCGGCGAAATCGGGTACCGCACAGGTTTACAGTTATGAAACCTATAATGCCAGT
AAAGTGGCAGAACACCTGCGCGACCATAAGTTAATGGTTGCGTTTGCTCCCTATGAGAACCCTACGGTATCCGTTGCAAT
AATCTTGGAAAATGGAGGCGCGGGGCCTGCGGTAGGGACCATTACCCGTCAGATCCTCGACCATATCTTGTTGGGCGATA
ATAATACTGAATTACCGGATGCGGTTCCTCTGCCTCCTGGCGTTGAAGCAGATTAG

Upstream 100 bases:

>100_bases
TCCTTATCACCGGGAATAAACGGGCGATGAAACTGAATCAAGTGATGGCTCTGGCTCTGAGTAAGATGCTGGTTTTGAGC
AAAATGACGTAAGCTGCGGA

Downstream 100 bases:

>100_bases
TTGAAACAGGTTAGTTGAAACAGATTAGTTGAAGCAGACTAAAAGCAAACTAATCGTTGATGCCCGTTGAGTTGAAATAG
GTCGCATTGAAGCCGATCAC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MKKEPNPFRDYSAESALFVRRAVVAFLGILLLSGVLVANLYNLQIVRFDDYSTRSNDNRIKLVPIAPSRGMIFDRNGTPL
ALNRTIYQLELMPEKIENLSATLNALRPIVDLTDDDIANFEKERKRSRRFTSIAVKTPLTEVQVARFAVNQFRFPGIEVK
GYQRRFYPYGSALTHVIGYVSKINDKDVERLDKEGILPNYAATHDIGKLGIERYYESTLHGKTGYEEVEVNNRGRVIRQL
HEQPPQAGKDIYLTLDLSLQIYIEKLLSGSRAAVVVTDPRTGGILALVSNPSYDPNLFVDGISNKDYQGLLNDTNRPLIN
RATQGVYPPASTVKPYIAVSALSAGVITKNTSLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTKALEESADTFFYQVAYDM
GIDRLSSWMSKFGYGEYTGIDLSEERAGLMPTREWKQKRHKKPWYQGDTIPVGIGQGYWTATPIQMAKALMTLINDGTVK
TPHLLQSTRIDGVLVPYKQEDSTQIGSINSGYWEIAKDGMYGVANRPNGTGRKFFEGTPYKAAAKSGTAQVYSYETYNAS
KVAEHLRDHKLMVAFAPYENPTVSVAIILENGGAGPAVGTITRQILDHILLGDNNTELPDAVPLPPGVEAD

Sequences:

>Translated_631_residues
MKKEPNPFRDYSAESALFVRRAVVAFLGILLLSGVLVANLYNLQIVRFDDYSTRSNDNRIKLVPIAPSRGMIFDRNGTPL
ALNRTIYQLELMPEKIENLSATLNALRPIVDLTDDDIANFEKERKRSRRFTSIAVKTPLTEVQVARFAVNQFRFPGIEVK
GYQRRFYPYGSALTHVIGYVSKINDKDVERLDKEGILPNYAATHDIGKLGIERYYESTLHGKTGYEEVEVNNRGRVIRQL
HEQPPQAGKDIYLTLDLSLQIYIEKLLSGSRAAVVVTDPRTGGILALVSNPSYDPNLFVDGISNKDYQGLLNDTNRPLIN
RATQGVYPPASTVKPYIAVSALSAGVITKNTSLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTKALEESADTFFYQVAYDM
GIDRLSSWMSKFGYGEYTGIDLSEERAGLMPTREWKQKRHKKPWYQGDTIPVGIGQGYWTATPIQMAKALMTLINDGTVK
TPHLLQSTRIDGVLVPYKQEDSTQIGSINSGYWEIAKDGMYGVANRPNGTGRKFFEGTPYKAAAKSGTAQVYSYETYNAS
KVAEHLRDHKLMVAFAPYENPTVSVAIILENGGAGPAVGTITRQILDHILLGDNNTELPDAVPLPPGVEAD
>Mature_631_residues
MKKEPNPFRDYSAESALFVRRAVVAFLGILLLSGVLVANLYNLQIVRFDDYSTRSNDNRIKLVPIAPSRGMIFDRNGTPL
ALNRTIYQLELMPEKIENLSATLNALRPIVDLTDDDIANFEKERKRSRRFTSIAVKTPLTEVQVARFAVNQFRFPGIEVK
GYQRRFYPYGSALTHVIGYVSKINDKDVERLDKEGILPNYAATHDIGKLGIERYYESTLHGKTGYEEVEVNNRGRVIRQL
HEQPPQAGKDIYLTLDLSLQIYIEKLLSGSRAAVVVTDPRTGGILALVSNPSYDPNLFVDGISNKDYQGLLNDTNRPLIN
RATQGVYPPASTVKPYIAVSALSAGVITKNTSLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTKALEESADTFFYQVAYDM
GIDRLSSWMSKFGYGEYTGIDLSEERAGLMPTREWKQKRHKKPWYQGDTIPVGIGQGYWTATPIQMAKALMTLINDGTVK
TPHLLQSTRIDGVLVPYKQEDSTQIGSINSGYWEIAKDGMYGVANRPNGTGRKFFEGTPYKAAAKSGTAQVYSYETYNAS
KVAEHLRDHKLMVAFAPYENPTVSVAIILENGGAGPAVGTITRQILDHILLGDNNTELPDAVPLPPGVEAD

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=630, Percent_Identity=80.7936507936508, Blast_Score=1058, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=636, Percent_Identity=24.0566037735849, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70530; Mature: 70530

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEPNPFRDYSAESALFVRRAVVAFLGILLLSGVLVANLYNLQIVRFDDYSTRSNDNRI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEE
KLVPIAPSRGMIFDRNGTPLALNRTIYQLELMPEKIENLSATLNALRPIVDLTDDDIANF
EEEEECCCCCEEECCCCCCEEECCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EKERKRSRRFTSIAVKTPLTEVQVARFAVNQFRFPGIEVKGYQRRFYPYGSALTHVIGYV
HHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHH
SKINDKDVERLDKEGILPNYAATHDIGKLGIERYYESTLHGKTGYEEVEVNNRGRVIRQL
HHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHH
HEQPPQAGKDIYLTLDLSLQIYIEKLLSGSRAAVVVTDPRTGGILALVSNPSYDPNLFVD
HHCCCCCCCEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEE
GISNKDYQGLLNDTNRPLINRATQGVYPPASTVKPYIAVSALSAGVITKNTSLFDPGWWQ
CCCCCCCHHHHCCCCCCHHHHHHCCCCCCHHHCCHHHEEHHHHCCEEECCCCCCCCCCEE
LPGSEKRYRDWKKWGHGRLNVTKALEESADTFFYQVAYDMGIDRLSSWMSKFGYGEYTGI
CCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHEEEEHHHHHCHHHHHHHHHHCCCCCCCCC
DLSEERAGLMPTREWKQKRHKKPWYQGDTIPVGIGQGYWTATPIQMAKALMTLINDGTVK
CCCHHCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
TPHLLQSTRIDGVLVPYKQEDSTQIGSINSGYWEIAKDGMYGVANRPNGTGRKFFEGTPY
CCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCCCCC
KAAAKSGTAQVYSYETYNASKVAEHLRDHKLMVAFAPYENPTVSVAIILENGGAGPAVGT
HHHCCCCCEEEEEEECCCHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEECCCCCCHHHH
ITRQILDHILLGDNNTELPDAVPLPPGVEAD
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKEPNPFRDYSAESALFVRRAVVAFLGILLLSGVLVANLYNLQIVRFDDYSTRSNDNRI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCEE
KLVPIAPSRGMIFDRNGTPLALNRTIYQLELMPEKIENLSATLNALRPIVDLTDDDIANF
EEEEECCCCCEEECCCCCCEEECCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EKERKRSRRFTSIAVKTPLTEVQVARFAVNQFRFPGIEVKGYQRRFYPYGSALTHVIGYV
HHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHH
SKINDKDVERLDKEGILPNYAATHDIGKLGIERYYESTLHGKTGYEEVEVNNRGRVIRQL
HHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHH
HEQPPQAGKDIYLTLDLSLQIYIEKLLSGSRAAVVVTDPRTGGILALVSNPSYDPNLFVD
HHCCCCCCCEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEE
GISNKDYQGLLNDTNRPLINRATQGVYPPASTVKPYIAVSALSAGVITKNTSLFDPGWWQ
CCCCCCCHHHHCCCCCCHHHHHHCCCCCCHHHCCHHHEEHHHHCCEEECCCCCCCCCCEE
LPGSEKRYRDWKKWGHGRLNVTKALEESADTFFYQVAYDMGIDRLSSWMSKFGYGEYTGI
CCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHEEEEHHHHHCHHHHHHHHHHCCCCCCCCC
DLSEERAGLMPTREWKQKRHKKPWYQGDTIPVGIGQGYWTATPIQMAKALMTLINDGTVK
CCCHHCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
TPHLLQSTRIDGVLVPYKQEDSTQIGSINSGYWEIAKDGMYGVANRPNGTGRKFFEGTPY
CCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCCCCC
KAAAKSGTAQVYSYETYNASKVAEHLRDHKLMVAFAPYENPTVSVAIILENGGAGPAVGT
HHHCCCCCEEEEEEECCCHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEECCCCCCHHHH
ITRQILDHILLGDNNTELPDAVPLPPGVEAD
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]