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The map label for this gene is miaB [H]

Identifier: 170025226

GI number: 170025226

Start: 3305244

End: 3306773

Strand: Direct

Name: miaB [H]

Synonym: YPK_3005

Alternate gene names: 170025226

Gene position: 3305244-3306773 (Clockwise)

Preceding gene: 170025223

Following gene: 170025227

Centisome position: 70.48

GC content: 48.37

Gene sequence:

>1530_bases
ATGCTCCCTAACGACGAAGCCTTTGCGACTCTCTACTACAACGGAGATTATCTACCACAACGGAGTACCGCACGGAACGA
GGTGATTTTGATGATGAATAACCCGATGACTAAAAAACTGCATATCAAAACCTGGGGTTGCCAGATGAATGAATACGATT
CATCTAAAATGGCAGATCTCTTGGCCAGTACCCATGGCTACCAGTTAACCACCATCCCGGAAGAAGCGGATTTACTGCTG
CTAAATACCTGTTCTATCCGGGAAAAAGCACAGGAAAAAGTATTCAGCCTGCTAGGGCAATGGAAATTATTGAAAGAGAA
AAACCCGCAGTTGATTATTGGGGTCGGTGGTTGCGTGGCGTCACAGGAAGGTGAACAATTGCGCCAGCGCGCCCCCTGTG
TCGATGTCATCTTCGGGCCACAAACGCTGCATCGTTTACCGGAAATGATTAACCACGTGCAAGAAACGAATAGCCCTGTC
GTGGATATCAGTTTTCCCGAAATTGAAAAATTTGACCGTTTGCCAGAACCACGGGCTGAAGGCCCAACTGCGTTTGTTTC
GATCATGGAAGGCTGCAATAAATATTGTACATTCTGTGTCGTACCCTACACCCGAGGCGAGGAAGTCAGCCGTCCAAGCG
ATGACATTCTTTTTGAAATCGCCCAGTTAGCCGCTCAAGGCGTACGTGAAGTCAACTTACTCGGCCAGAATGTTAACGCC
TACCGCGGGGCAACCTACGATGGCGATATCTGTAGTTTTGCCGAGCTGTTGCGTTTGGTTGCTGCCATTGATGGTATCGA
TAGGATACGATTTACCACCAGCCACCCCATTGAGTTTACTGACGATATCATTGATGTTTATCGCGATACGCCTGAATTGG
TGAGCTTCCTGCATTTGCCGGTACAAAGCGGTTCTGACCGTATTTTGACCATGATGAAGCGTGCCCATACCGCCCTGGAA
TACAAAGCCATCATCCGCAAACTGCGTCAGGCGCGCCCTGATATCCAGATAAGTTCTGATTTTATTGTCGGCTTCCCTGG
TGAAACCCAGCAGGATTTTGAACAGACCATGAAACTGGTCGCAGATATTCATTTTGATACCAGCTACAGCTTTATTTACT
CCCCGCGTCCCGGCACACCAGCGGCTGATTTACCCGACAATGTGTCCGAAGAGGAGAAAAAACAGCGGCTACATATCCTG
CAACAACGCATTAGCCAACAGGCGATGGAAATTAGCCGTAAGATGGTAGGGACAGTACAGCGTGTTTTGGTGGAGGGGAC
CTCTCGCAAAAATGTCATGGAACTGGCCGGGCGTACGGAAAACAACCGCGTCGTGAACTTTGAGGGTTCACCCGACATGA
TTGGTAAATTCGTTGATGTCGAAATTGTTAACGTTTATGCCAGTTCACTGCGCGGCATTTTACTGCGTACAGAAGATCAG
ATGGACCTGCGAACCCACGAGTCACCGCAATCGGTGATTGCTCGCACCCGTAAAGAAAATGAAATTGGTGTAGGTATTTA
TCAGCCTTAA

Upstream 100 bases:

>100_bases
TTAGCAAGGTTTTGTCTAACCGCAGTGTAACGGATTTTTTATCACTCAACAGAATAACTCAACGGTTCTGCGGTGGTCAT
CGCAGAGGCAAATGACTACA

Downstream 100 bases:

>100_bases
CCAACATCCATTTTGGTAACGCCCGGCCTAACATCATTGATCTGACAGGTTTACTTGATCCCGCCCGCGTAACGAGGCAC
TGCATTGTCAGTGCCTGTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLL
LNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQETNSPV
VDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNA
YRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALE
YKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHIL
QQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQ
MDLRTHESPQSVIARTRKENEIGVGIYQP

Sequences:

>Translated_509_residues
MLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLL
LNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQETNSPV
VDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNA
YRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALE
YKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHIL
QQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQ
MDLRTHESPQSVIARTRKENEIGVGIYQP
>Mature_509_residues
MLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLL
LNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQETNSPV
VDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNA
YRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALE
YKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHIL
QQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQ
MDLRTHESPQSVIARTRKENEIGVGIYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=35.8921161825726, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI28872782, Length=492, Percent_Identity=35.7723577235772, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI93277076, Length=448, Percent_Identity=30.1339285714286, Blast_Score=181, Evalue=2e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=83.9662447257384, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=404, Percent_Identity=30.4455445544554, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=32.3275862068966, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=36.5079365079365, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI19922432, Length=465, Percent_Identity=30.1075268817204, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 57538; Mature: 57538

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADL
CCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHH
LASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVA
HHHCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
SQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQETNSPVVDISFPEIEKFDRLPEPRAE
CCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCC
GPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNA
CHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
YRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPELVSFLHLP
HCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHH
VQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHH
ADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQ
HHHEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQ
HHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
MDLRTHESPQSVIARTRKENEIGVGIYQP
CCCCCCCCHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MLPNDEAFATLYYNGDYLPQRSTARNEVILMMNNPMTKKLHIKTWGCQMNEYDSSKMADL
CCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHH
LASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVGGCVA
HHHCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
SQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQETNSPVVDISFPEIEKFDRLPEPRAE
CCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCC
GPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNA
CHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
YRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHPIEFTDDIIDVYRDTPELVSFLHLP
HCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHH
VQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQTMKLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHH
ADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQ
HHHEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQ
HHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCEEEEECCCC
MDLRTHESPQSVIARTRKENEIGVGIYQP
CCCCCCCCHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA